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This protocol describes a humanized mouse model created using neonatal thymus and umbilical cord blood hematopoietic stem cells as nonfetal human tissue sources.
A clinically relevant murine model of septic arthritis via direct inoculation of methicillin-resistant Staphylococcus aureus into the knee joint is described. This protocol details methods for serum, synovial fluid and knee joint tissue analysis that more closely mimic the workup of septic arthritis in human patients.
This protocol describes an in vivo cartilage formation assay. Human or bovine articular chondrocytes injected in nude mice form cartilage organoids that can be used for the screening of molecules that promote cartilage formation.
This protocol details the procedures for safe cardiac catheterization in Landrace pigs, basic electrophysiological assessment of conduction properties, induction of atrial fibrillation and development of a myocardial infarction model.
This protocol details the fabrication and application of a 3D-printed device for facilitating lateral tail-vein injection in mice. The tail vein–illuminating ring comprises a light-emitting diode (LED) lamp box on the side; once the tail has been illuminated by the LED, the vein is more clearly visible along the lateral side of the mouse tail.
A new rat model of brainstem ischemia is described. Selective ligation of four points of the lower basilar artery causes a localized brainstem ischemic lesion in adult rats, resulting in hemiparesis, as well as abnormal posture, body balance and locomotion.
This protocol describes the different steps to establish a murine model of restenosis after angioplasty of a stenotic arteriovenous fistula (AVF), including a detailed description of the partial nephrectomy procedure to induce chronic kidney disease, the AVF procedure for development of de novo stenosis and the angioplasty treatment associated with progression of restenosis.
Goblet cell–associated antigen passages can deliver luminal substances to antigen-presenting cells to induce antigen-specific T cell responses. This protocol describes how to identify and quantify intestinal epithelial cells that have the capacity to take up luminal substances, by intraluminal injection of fluorescent dextran, tissue sectioning for slide preparation and imaging with fluorescence microscopy.