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This protocol describes the generation of single-clone viral stocks from patients with severe acute respiratory syndrome coronavirus 2, from the isolation of samples from patient swabs to the purification of specific clones, quality control and the generation of working stocks.
Combinatorial fluorescent labeling of anaerobic (and aerobic) bacterial strains using azide-modified sugars for macromolecular synthesis and a biorthogonal reaction for the cycloaddition of multiple fluorophores, suitable for use in anaerobic conditions.
There is increasing concern about the harmful effects of micro- and nanoplastics (MNPs). This protocol describes how to generate MNP samples and perform ecotoxicity experiments by taking into account their physicochemical properties and behavior.
An integrated workflow for multiplexed tissue image processing and analysis, including interactive inspection of raw data, cell segmentation, feature extraction, single-cell analysis and spatial analysis.
The authors provide an expanded CRISPR–Cas9-assisted recombineering toolkit for rapid and efficient engineering of genetically intractable Pseudomonas aeruginosa isolates.
The authors describe a new base editing system—the transformer base editor—to induce efficient editing with no observable genome-wide or transcriptome-wide off-target mutations, both in mammalian cells and in mice.
Microbial pathogens develop resistance to clinically used drugs, and finding new therapeutics is an ongoing challenge. The photoimmuno-antimicrobial strategy described in this protocol is a general approach to target specific elimination.
Invasion–adhesion-directed expression sequencing adapts the 10x Genomics 5′ single-cell RNA sequencing protocol to enable generation of bacterial and eukaryotic DNA libraries to identify adherent or invasive bacteria and the associated host transcriptome at a single-cell level.
The authors provide a protocol to microscopically identify and isolate single maize meiocytes and pollen grains for RNA-seq with a modified version of CEL-Seq2. Staging of developmentally synchronized anthers is performed in parallel with expression analysis.
This protocol can be used to generate kidney organoids with elongated proximalized nephrons displaying improved proximal tubule maturity compared with those generated using standard kidney organoid protocols.
Procedures for the synthesis of physical double-network hydrogels to provide self-healing, pH-responsive, tissue-adhesive, antioxidant, photothermal and antibacterial properties. Used as wound dressings, the hydrogels can also be removed on demand.
This Tutorial provides guide for how to evaluate, select and use publicly available computational tools for predicting intrinsic disorder in proteins, with a focus on performance and ease of use results, exemplified using results from the Critical Assessment of protein Intrinsic Disorder prediction experiment.
This protocol provides structure-guided capsid library approaches to evolve new adeno-associated viral vectors for enhanced CNS gene delivery with altered tissue tropism, higher transduction efficiency and the ability to evade pre-existing humoral immunity.
This protocol describes how to prepare ordered mesoporous intermetallic nanomaterials with controlled compositions, morphologies/structures and phases via a general concurrent template strategy.
We provide a Perspective highlighting the significant ethical implications of the use of fast-developing neurotechnologies in humans, as well as the regulatory frameworks and guidelines needed to protect neurodata and mental privacy.
Purifying proteins by crystallization rather than chromatography is less expensive and more environmentally sustainable. This protocol describes how to determine the operational conditions for purifying monoclonal antibodies by template-assisted membrane crystallization.
This Protocol documents the extensive preparation required to adopt Neuropixels probes for use in patients for recording spiking activity from up to hundreds of cortical neurons and associating it with local field potentials.