Correction to: Nature Protocols https://doi.org/10.1038/s41596-022-00730-6, published online 24 August 2022.

In the version of the article initially published, in the “Open-source microscopies and components for single-molecule imaging” section, the “Hohlbein laboratory” incorrectly appeared as “Holbhein” and the sentence “The miCube can perform 3D single molecule imaging and single molecule FRET measurements and can be combined with a flat-field imaging module” was added alongside four new references. References 17–20 are now: Martens, K. J. A., Bader, A. N., Baas, S., Rieger, B. & Hohlbein, J. Phasor based single-molecule localization microscopy in 3D (pSMLM-3D): An algorithm for MHz localization rates using standard CPUs. J. Chem. Phys. 148, 123311 (2018); Martens, K. J. A., Jabermoradi, A., Yang, S. & Hohlbein, J. Integrating engineered point spread functions into the phasor-based single-molecule localization microscopy framework. Methods 193, 107–115 (2021); Martens, K. J. A. et al. Enabling spectrally resolved single-molecule localization microscopy at high emitter densities. Nano Lett. 22, 8618–8625 (2022) and Jabermoradi, A., Yang, S., Gobes, M. I., van Duynhoven, J. P. M. & Hohlbein, J. Enabling single-molecule localization microscopy in turbid food emulsions. Phil. Trans. R. Soc. 380, 20200164 (2022). Additionally, in Table 1, cells in the miCube column for the rows “Flat-field illumination”, “Multi-color imaging” and “3D imaging” now read “Yes” rather than “No”. These changes have been made to the HTML and PDF versions of the article.