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We engineered integrase-deficient lentiviruses to act as vectors for the delivery of large gene knock-ins via homology-directed repair. This technology enables the non-cytotoxic, targeted insertion of difficult-to-express transgenes into genomic loci that are essential to cell survival, thereby overcoming the gene silencing that otherwise limits primary immune cell engineering.
We developed exponentially amplified rolling circle amplification with CRISPR–Cas12a as a one-pot, isothermal assay for microRNA detection. This method has single-digit femtomolar sensitivity and single-nucleotide specificity, and can be deployed for point-of-care testing. The assay has been adapted for the microRNA profiling of extracellular vesicles, which is used in the diagnosis of pancreatic cancer.
INSPECTR is a technique for detecting nucleic acids that couples the sensitivity and specificity of nucleic acid splinted ligation with the versatile readouts of cell-free gene expression. The result is an ambient-temperature workflow that enables the detection of pathogenic viruses at low copy numbers.
This Review discusses the characteristics of neural probes that are most likely to facilitate the clinical translation of invasive neural interfaces, the abiotic and biotic factors that contribute to their failure and emerging neural-interface architectures.
Confining the Zika virus within a self-adjuvanting hydrogel that forms a subcutaneous inflammatory niche stimulates strong immune responses in mice without inducing infection.
In this study, spherical nucleic acids (SNAs) were designed using defined placements of two classes of antigen that each activate different types of T cell. Use of SNA modularity illustrated the role of antigen placement within a vaccine architecture on subsequent processing and immune responses. The study defines design rules for multi-antigen vaccines.
We developed an at-home microsampling approach that measures thousands of metabolites, lipids and proteins in small volumes of blood. Dense multi-omic sampling generates a ‘molecular movie’ that integrates with data from wearables to reveal new insights into the dynamics of human physiology.
This Perspective argues that dermal interstitial fluid cannot be generally considered a diagnostically useful proxy for blood, yet that it can offer advantageous utility for the monitoring of therapeutic drugs and of the status of the immune system.
This Review discusses requirements—in cell sources, functional and safety testing, manufacturing and storage, and clinical-trial design—for the clinical advancement of cell therapies for Parkinson’s disease.
An extracellular matrix biomaterial delivered into the bloodstream selectively binds to blood vessels in inflamed tissues, such as those caused by myocardial infarction and traumatic brain injury. The biomaterial dampened the inflammatory response and promoted tissue repair and regeneration when tested in rat and pig models of myocardial infarction.
This Perspective overviews the sources of prediction uncertainty in machine learning for applications in healthcare, and discusses how to implement suitable prediction-uncertainty metrics.
Antitumour responses mediated by T helper 17 cells can be induced by intratumourally injected hollow nanoparticles displaying the polysaccharide mannan.
Each of the 13 protein-coding genes in the mouse mitochondrial genome can be ablated using a library of optimized double-stranded-DNA deaminase-derived cytosine base editors.
The targeted delivery of the short-chain fatty acid butyrate to the lower gastrointestinal tract by polymeric nanoscale micelles restores intestinal homeostasis in mouse models of peanut allergy and colitis.
Liposomal nanoparticles incorporating photosensitive lipids and enclosing paramagnetic molecules enable the mapping, via magnetic resonance imaging, of spatial variations of light intensity in illuminated brain tissue in living animals.
This Review highlights technological advances in exoskeleton technology from the past two decades, and challenges and opportunities in the exoskeleton-assisted rehabilitation of gait.
Pooled testing for the diagnosis of COVID-19 via isothermal nucleic acid amplification and detection can be automated by using electromagnetically actuated swarms of millimetric magnets to handle droplets of magnetized samples on a microfluidic chip.
