RNA modifications

Direct RNA-seq offers the possibility to identify RNA modifications on single molecules. Here, the authors report on the synthesis of biologically realistic training data and the development of mAFiA that accurately detects m⁶A on single read level.

N6-methyladenosine (m6A) maintains maternal RNA stability in oocytes. Here, the authors identify demethylase ALKBH5 as a key determinant of oocyte quality and unveil the facilitating role of ALKBH5-mediated m6A removal in maternal RNA decay.

m⁶A mRNA modification is not typically found near splice junctions in mRNAs. Here the authors show exon-intron boundary inhibits m6A deposition at ~100 nt region nearby splice site, enabling m⁶A distribution hallmark, more stable mRNA and flexible protein coding.

The current available tools lack the ability to accurately classify and visually represent epitranscriptomic profiling data. Here, the authors provide a framework that offers a general solution for the visualization and interpretation of such data.