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Off-target effects of programmable nucleases remain a critical issue for therapeutic applications of genome editing. This review compares experimental and computational tools for off-target analysis and provides recommendations for better assessments of off-target effects.
In this Perspective, the authors discuss strategies for creating a versatile set of 30 genetically engineered mouse models (GEMMs) useful for coronavirus disease 2019 research. In addition, they provide the genetic blueprints needed for developing these GEMMs.
This tutorial describes how to design nanoparticle-based LFAs for detecting biomolecules. The authors provide guidance on how to select the appropriate lateral-flow strip components and bioreceptors as well as detection strategies.
This tutorial describes a set of essential performance tests for characterization of neural interface electrodes. The authors provide guidelines for standardized implementation and reporting on electrode performances.
Neural interfaces with implantable electrodes are used to modulate and restore function to the peripheral nervous system. Hybrid modeling described in this protocol is used to optimize each aspect of the implantable electrode design and operation.
This tutorial describes the main causes of sample-induced spherical aberration artifacts in 3D microscopy. In addition, the authors provide a practical ImageJ/Fiji-based solution to correct these aberrations during or after image acquisition.
In this review, the authors present comprehensive guidelines for performing and evaluating PRS analyses. This is accompanied by an introductory online tutorial that takes users through quality control and visualization steps.
A tutorial for multivariate classification analysis of vibrational spectroscopy data (Fourier-transform infrared, Raman and near-IR) is presented. Guidelines are provided for data preprocessing, data selection, feature extraction, classification and model validation.
This tutorial and the accompanying poster (https://doi.org/10.1038/s41596-020-0307-7) provide a guide for performing quantitative fluorescence imaging using confocal microscopy. It includes advice and troubleshooting information from sample preparation and microscope setup to data analysis and statistics.