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Detailed guides for the husbandry of Xenopus laevis and protocols for making reproducible spinal cord injury models and subsequent study of neural stem/progenitor cells during regeneration are described.
This protocol provides details for dispersion and colloidal characterization of suspended engineered nanomaterials, and computational fate and transport modeling to accurately calculate dose metrics for in vitro cellular nanotoxicology experiments.
ClusPro is a web server that performs rigid-body docking of two proteins by sampling billions of conformations. Low-energy docked structures are clustered, and centers of the largest clusters are used as likely models of the complex.
The metabolome or lipidome of different phenotypes can be compared using this protocol. A complete workflow for direct-infusion mass spectrometry using nanoelectrospray ionization and spectral stitching with the Galaxy platform is described.
This protocol from Wang et al. describes a pulse–chase method to investigate autophagic protein degradation through click labeling of long-lived proteins. This is a safer alternative to similar classic methods that use radioactive labeling.
Friedrich et al. describe their toolkit for transposon-based insertional mutagenesis in mice for discovering cancer genes. Genome-wide transposon insertion sites are identified, mapped and quantified using QiSeq.
This protocol extension describes the fabrication of optofluidic neural probes and implantation for advanced in vivo pharmacology and optogenetics in freely moving rodents.
Moreno-Romero et al. describe how to apply the INTACT method to purify endosperm nuclei from Arabidopsis. Purified nuclei can be used for ChIP-seq analysis or bisulfite sequencing.