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Label-free and reagentless analysis of biomarkers is ideal for point-of-care diagnostics. In this protocol, changes in electrochemical capacitance resulting from biomarker binding to an electrode coated with a biological receptor are detected.
This protocol describes how to generate oligodendrocyte precursor cells from human pluripotent stem cells that can subsequently myelinate neurons, both in vitro and in vivo.
This protocol assesses proinflammatory properties of nucleic acid nanoparticles (NANPs) in PBMCs, which are highly predictive of cytokine responses. The authors detail how to prepare NANPs and analyze characteristic biomarkers produced by PBMCs transfected with NANPs.
RepeatExplorer is a software tool for repeat identification and quantification using unassembled sequencing reads. The authors describe four pipelines implemented on the Galaxy platform, highlighting different applications.
This protocol describes how to record intracellular neuronal activity in awake nonhuman primates in response to external stimuli. With the help of a coaxial guide tube, multiple neurons can be recorded over longer times from a single individual.
Here, the authors describe step-by-step procedures for integrating single-cell sequencing datasets from different experiments or modalities to identify common and distinct cell types using the R-based software tool LIGER.
This protocol describes the light-induced functionalization of proteins with compounds bearing the photochemically active aryl azide group. The procedure is exemplified by the light-induced radiosynthesis of zirconium-89 radiolabeled mAbs.
The authors describe how to easily prepare a large number of dipsticks from cellulose-based filter paper and use them to rapidly purify nucleic acids from a variety of sources.
The authors describe an approach for optimizing transcranial magnetic stimulation (TMS) targeting by combining functional magnetic resonance imaging and iterative electric-field stimulation.
This protocol describes a comprehensive computational pipeline for reference-free deconvolution of bulk DNA methylation data, including data preprocessing, confounder adjustment, feature selection, and visualization and interpretation of the results.
The production and titration of the SARS-CoV-2 S pseudotyped virus using a VSV-based pseudovirus production system in this protocol enable its use under biosafety level 2 conditions as well as in a neutralization assay to assess the level of neutralizing antibodies or molecular inhibitors in a sample.
Solid acid catalysts are used in a wide variety of industrial catalytic processes for production of chemicals and petrochemicals. This protocol uses 31P NMR of phosphorous probes to characterize acidity in detail for both Brønsted and Lewis acid sites.
This protocol describes the synthesis of two fluorescent probes for (sub-)cellular detection of endogenous formaldehyde, including procedures for probe characterization and example applications in living cells and mouse tissue slices.
This homology-directed insertion-based CRISPR gene-editing protocol enables knockout of all alleles of a target gene in the polyploid Drosophila S2R+ cell line, using either two sequential rounds of homology-directed insertion or a single round with a donor vector containing four different sgRNAs.
This protocol summarizes the various approaches available to derive organoids from cancer patients and use these for screening of possible treatments. An optimized protocol for using head and neck cancer organoids is also described.
The authors describe a streamlined epigenomic profiling protocol based on cut-and-paste tagmentation by the Tn5 transposase targeted to a chromatin protein of interest.
This protocol describes a combined approach for whole-mount fluorescence in situ hybridization (FISH) and immunofluorescence staining in zebrafish embryos and larvae.
This protocol describes how to isolate trophoblast from first-trimester human placentas and grow it long term in a three-dimensional organoid culture system.
This protocol describes a toolbox for comprehensive characterization of inflammasome activation and cell death in response to both in vivo (in mice) and in vitro (using bone marrow–derived macrophages) models of infection, sterile insults, and cancer.
The authors describe a standardized three-chamber social preference protocol that is sensitive and reliable at detecting social preference deficits in several mouse models of autism spectrum disorder.