Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain
the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in
Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles
and JavaScript.
The authors describe a technique for reprogramming human somatic cells into induced trophoblast stem cells and the conversion of human naive and extended pluripotent stem cells into converted trophoblast stem cells, with functional assays for their differentiation potential.
Protocol for isolating ready-made rat microvessels from adipose tissue and their incorporation into 3D collagen hydrogels. These microvessel gels can be used for in vitro angiogenic assays or transplanted into mice for in vivo vascularization studies.
This protocol describes proximity biotinylation in cells without transfection or transduction using an off-the-shelf recombinant ProtA-TurboID enzyme consisting of a biotin ligase fused to an antibody-recognizing moiety, which, combined with a bait-protein-specific antibody, can be used to identify bait-proximal proteins.
This protocol provides an enzyme-free method to achieve targeted DNA demethylation based on steric hindrance by dCas9 bound at or close to CpG sites of interest to identify DNA methylation marks causal to altered gene expression.
Inhibitors for carbohydrate digestive enzymes are used to manage conditions like diabetes. In this assay for digestive enzyme activity, sugars are quantified by using high-performance anion-exchange chromatography with pulsed amperometric detection.
Multispectral large-scale single-cell resolution 3D imaging allows up to eight fluorophores to be captured in a single acquisition. This protocol enables the visualization and exploration of large intact tissue volumes.
The authors provide a guide to using the Kraken suite for metagenomics analysis, including classification, quantification and visualization, illustrated by quantification of species in the microbiome and identification of pathogens in a clinical sample.
This two-stage bioreactor-mediated approach for culturing previously uncultured microorganisms involves enrichment of indigenous organisms by using a continuous-flow down-flow hanging sponge bioreactor and subsequent selective batch cultivation.
Little is known about the brain mechanisms of hallucinations. This protocol describes the use of a robotic device and sensorimotor method to reproducibly induce and measure presence hallucinations by behavioral assay and fMRI.
Direct carbonization of zeolitic imidazolate framework-type metal-organic frameworks, as described in this protocol, results in nanoporous carbons that can be used for diverse electrochemical applications.
The cell membrane turnover kinetics of the main glial glutamate transporter GLT1 are quantified by fusing it to a pH-sensitive fluorescent protein and performing measurements using whole-cell fluorescence recovery after photobleaching.
Photochemical methods are increasingly being used in chemical synthesis. This protocol describes how to optimize the reaction conditions for a standard chiral example using cyclodextrin derivatives as supramolecular hosts.
This protocol provides a step-by-step guide to building an affordable single-molecule localization microscopy setup for high-throughput super-resolution imaging by using off-the-shelf and machined parts.
This protocol includes a suite of procedures for measuring the passive permeability of solutes through membranes using fluorescence-based assays that report either volume or pH changes of synthetic vesicles or live cells.
Identifying metabolites in cells of the tumor microenvironment is an area of intense study. This protocol describes specimen collection, enrichment of human immune cell populations and analysis by flow cytometry and LC–MS.
Small-animal blood exchange replaces or dilutes blood components more precisely than parabiosis does. This protocol describes the exchange process, provides blueprints of the required device and derives equations for simulating dilution of biomolecules.
Volcano plots and activity maps are powerful tools for studying homogeneous catalysis. This protocol describes how to build these from computed DFT data and use them to predict and rationalize the performance of homogeneous catalysts.
This protocol uses signaling networks and molecular markers recently elucidated from single-cell transcriptomic analysis of mouse foregut organogenesis to differentiate human pluripotent stem cells into digestive and respiratory organ-specific mesenchyme.
Contamination of water by oil is a problem both in the environment and in engineering applications. This protocol describes how to prepare a transparent superoleophobic film that can coat lenses or make separation membranes for use underwater.
This protocol describes the isolation from brain tissue of extracellular vesicle subpopulations, including microvesicles, exosomes and mitochondria-derived mitovesicles, using a high-resolution (iodixanol) density step gradient. Extracellular vesicle characterization and analysis are also presented.