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WISH-tags can be used in combination with quantitative polymerase chain reaction or next-generation sequencing to decipher population dynamics at the strain level within plant and mammalian microbiotas.
Testing 255 combinations of chemicals versus bacterial communities shows species diversity contributes to resilience against increasingly complex stressor mixtures.
Cryogenic electron microscopy analysis of the Mycobacterium tuberculosis membrane-bound phosphoribosyltransferase Rv3806c provides mechanistic insight into cell wall precursor synthesis.
Single-molecule imaging reveals that peptidoglycan synthesis and synthase activity, rather than FtsZ treadmilling, are rate limiting and drive septum constriction in Staphylococcus aureus.
Live-cell single-molecule imaging reveals a single population of processive septal peptidoglycan synthases moving asynchronously with FtsZ that drive Bacillus subtilis cell constriction in a manner partially dependent upon FtsZ treadmilling.
Cryoelectron microscopy analyses of the counter-clockwise and clockwise states of the Salmonella Typhimurium C-ring reveal the structural bases for changes in rotation of the bacterial flagellum.
Experimental evolution of Pseudomonas aeruginosa shows that the population is more vulnerable to invading cheaters in a spatially extended system due to a higher level of cooperation.
The bacterial alarmone (p)ppGpp induces β-lactam resistance through modification of RNA polymerase and ribosome function rather than regulation of peptidoglycan metabolism in Escherichia coli.
Biochemical, genetic and structural analyses show how phage ΦKZ co-opts the Pseudomonasaeruginosa gene expression machinery using a factor that binds to host ribosomes at 5S ribosomal RNA.
During archaeal cell division, proteins containing photosynthetic reaction centre domains enable the formation of a defined cell division plane by direct interaction with SepF.
Metabolic engineers and synthetic biologists can produce recombinant proteins at scale without royalty payments, or strain distribution constraints, using OPENPichia.
A set of computational tools to de novo recognize plasmids in complex environments, like the human gut microbiome, and to organize them into evolutionarily cohesive units.
Photosynthesis reaction centre barrel proteins are important components of the FtsZ-based cell division apparatus in Haloferax volcanii and other archaea.
Graft-versus-host disease, a T cell-driven inflammatory condition, is associated with altered microbial bile acid metabolism in both mice and humans and this is linked to outcomes.
A 5 year longitudinal, retrospective and multi-centre epidemiological study of carbapenem-resistant Klebsiella pneumoniae across China reveals antibiotic resistance, virulence gene and capsule profiles for 1,017 isolates showing increased K64 prevalence, increased hypervirulence and moderate frequency, stable circulation of capsule- or O-antigen-deficient strains.
Autophagy in CD11c+ phagocytes prevents myeloid-derived suppressor cell recruitment and promotes protective T cell responses during high-dose Mycobacterium tuberculosis infection in mice.
Bacteria cooperate by secreting siderophores that displace iron from the antibiotic cefiderocol, reducing drug uptake and promoting the cross-protection of susceptible siblings and other species.
The Candida albicans toxin, candidalysin, is embedded in an unusual conserved precursor peptide sequence (Ece1). The precursor is not required to block premature pore-forming toxicity, but rather to prevent candidalysin auto-aggregation.