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A Xenopus embryo coinjected with a plasmid encoding a transgene and the φC31 integrase mRNA readily facilitates genomic integration resulting in healthy transgenic embryos.
The pathogenic arsenal of many bacteria includes an apparatus that mediates the injection of a cocktail of virulence proteins directly into host cells. Spatiotemporal aspects of this process can now be analyzed in living cells.
Understanding neuronal integration comes a step closer to reality with the development of a crystal-based, beam-steering microscope for uncaging neurotransmitters, which will permit experimental interrogation of the spatiotemporal interactions between the thousands of synapses a neuron receives.
Since the 1970s, fluorescence recovery after photobleaching has advanced our understanding of cell membrane dynamics and cytoplasmic signaling pathways. This technique has now been applied in the nucleus to address questions in epigenetics and provides a useful new tool to develop pharmacotherapies for human disease.
The Sleeping Beauty (SB) transposon emerged as a useful tool for applications such as germline and somatic cell insertional mutagenesis and now shows its usefulness again by facilitating saturating germline mutagenesis in mice.
How sure can we be to have identified the right proteins in a large scale proteomics study with our mass spectrometric instrumentation? Can we expect valid data from the employed search algorithm(s)? Can we believe what our computer is telling us? Right questions—what are the answers?
In this issue a review and a protocol describe advances in applying amperometry to biology. Here we provide an overview of amperometry's origins and how it is used to examine the basics of exocytosis.
With the advent of microfluidics technology and the development of a user-friendly device, studying high-density colonies of microorganisms in controlled chemostatic conditions now becomes a reality.
Targeted genomic insertion will improve the qualitative and quantitative functional comparison of similar transgenes and provide suitable integration points for transgenes of applied interest.
Solution polymers such as dendrimers promise to provide valuable new tools for proteomics researchers using mass spectrometry (MS) to probe protein modifications.
The nonphototoxic nature of GFP makes it an excellent imaging probe but a poor tool for techniques that rely on generation of toxic radicals such as chromophore-assisted light inactivation (CALI). Multiphoton excitation helps overcome these limitations.