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12 microRNA expression profiling platforms are compared for their reproducibility, sensitivity, accuracy and specificity, and the strengths and weaknesses of each platform are discussed.
This paper demonstrates that micropatterned human embryonic stem cell colonies can acquire spatial patterns reminiscent of those in the embryo and proposes their use to study early developmental processes in the human system.
The results of genome-wide association studies are combined with quantitative interaction proteomics to narrow down the list of putative causal disease genes and filter modest association signals.
A combination of a sensitive blue light–gated channelrhodopsin actuator and red-shifted Arch-based voltage sensors allows all-optical electrophysiology without cross-talk in cultured neurons or brain slices.
A simple, robust, chemically defined method for generating cardiomyocytes from human pluripotent stem cells is described. It should enable the identification of conditions for maturation of these cells.
A tetramethylrhodamine-labeled dimer of the cell-penetrating peptide TAT, named dfTAT, efficiently delivers proteins into live cells by facilitating endosomal escape.
This paper describes a single-vector strategy for flexible intersectional expression of genetically encoded reporters and tools in precisely defined cell types in the mouse.
A single-cell Western (scWestern) blotting technique allows quantitative measurements of up to 11 protein targets from ~2,000 individual cells in under 4 hours, expanding single-cell heterogeneity studies to the proteome.
The transformation of individual animal images acquired from videos into unique reference fingerprints allows for robust tracking of individuals in groups and reidentification of individuals between sightings and across different videos.
Far-red fluorogenic probes for live-cell imaging of either actin or tubulin are described and used for super-resolution microscopy of various structures in a variety of cell types.
This paper reports the use of light-field microscopy for fast, large-scale imaging of neuronal activity in vivo. It is applied to image the entire animal in the worm and the whole brain in zebrafish.
By combining the use of relatively large crystals and an X-ray free-electron laser, a radiation damage–free three-dimensional structure of a radiation-sensitive protein (bovine cytochrome oxidase) was solved at 1.9-Å resolution.
This paper combines cryo-electron tomography with super-resolution fluorescence microscopy for precise localization of molecular tags on a cellular or macromolecular structure, without fixation.
The high-throughput sequencing–RNA affinity profiling (HiTS-RAP) assay enables large-scale profiling of protein interactions with RNA libraries using a simple protocol on a high-throughput sequencer.
Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9–based transcriptional repressors can be easily engineered to give rise to a large library of orthogonal devices for complex circuits in mammalian cells.
A two-step error correction process for high throughput–sequenced T- and B-cell receptors allows the elimination of most errors while not diminishing the natural complexity of the repertoires.
Bone marrow formed in a cylindrical PDMS device implanted in a mouse can be surgically removed and cultured for a week in vitro without losing any of the hallmarks of in vivo bone marrow niches.
Light-activated reversible inhibition by assembled trap (LARIAT) is a versatile optogenetic method for inactivating proteins, including GFP fusions, in living cells by conditional clustering at high spatiotemporal resolution.