Reviews & Analysis

Advances in parallelization allow a human genome to be sequenced using single-molecule technology.

The massive capacity of today's sequencing machines can be harnessed efficiently by sequencing pooled samples and decoding the results.

Systemic delivery of an siRNA–aptamer chimera leads to prostate cancer regression in mice.

A group of scientists in the systems biology community propose visual conventions for drawing biological diagrams.

Unstructured translation-initiation sequences enable protein synthesis in cell extracts from multiple organisms.

Tandem gene duplication is harnessed to genetically engineer Escherichia coli, enabling sustained expression of metabolic products.

Controlling the spatial organization of sequential metabolic enzymes may provide a general strategy for increasing the production of valuable compounds.

Quantum dots that are small and non-blinking offer new opportunities for dynamic single-molecule imaging in live cells.

Now that ~1,000 microbial genomes have been sequenced, Nikos Kyrpides reflects on the past decade of microbial genomics and extrapolates forward to propose solutions to meeting challenges in the field.

A multilaboratory study demonstrates the potential for establishing quantitative targeted proteomic assays for moderately to highly abundant plasma proteins.

A near-infrared fluorescent protein opens a window into the mammalian body.

Bacterially derived minicells loaded with siRNA reverse drug resistance in tumor xenografts.

Addition of a photodegradable group to the backbone of synthetic hydrogels enables real-time control of the material's chemical and physical properties.

Biological validation of a cadre of new obesity genes supports the power of studies that exploit 'expression quantitative trait loci'.

Crystal structures of a mammalian multidrug efflux pump bound to peptide inhibitors may reveal drug-binding sites.

Antisense oligomers targeted to CAG repeats allow allele-specific knockdown of the gene that causes Huntington's disease.

Synthetic gene networks can be readily redesigned using new libraries of quantitatively characterized promoters coupled with predictive mathematical modeling.

Antibodies can be programmed to bind cancer cells using covalently binding antigens and adapters.

Mapping the vast quantities of short sequence fragments produced by next-generation sequencing platforms is a challenge. What programs are available and how do they work?

The MoBY-ORF collection of barcoded yeast genes provides mechanistic insights into antiproliferative compounds.