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Data availability
The image data used to generate the correction matrices for the 24-well plate adapter are available upon request. The calculated correction matrices are available online at https://www.github.com/WeberSynBioLab/optoConfig-96.
Code availability
optoConfig-96 is available at https://www.github.com/WeberSynBioLab/optoConfig-96, along with the most up-to-date version of the user guide (Supplementary Manual).
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Acknowledgements
We thank J. Schmidt, D. Renz and L. Breh (technical workshop of the Faculty of Biology, University of Freiburg, Germany) for assembling the optoPlates-96 and 3D printing of the 24-well plate adapter. We also thank A. Fischer, C. Jerez-Longres, L. Lataster, H. Mohsenin and N. Urban for testing optoConfig-96. This work was supported in part by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy CIBSS–EXC-2189, Project ID 390939984. This work was supported in part by the Excellence Initiative of the Deutsche Forschungsgemeinschaft (GSC–4, Spemann Graduate School) and in part by the Ministry for Science, Research and Arts of the State of Baden-Wuerttemberg.
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O.S.T., M.H. and W.W. conceptualized optoConfig-96. O.S.T. wrote the software. M.H. designed and characterized the 24-well plate adapter. M.H. and W.W. supervised the project. O.S.T., M.H. and W.W. wrote and edited the manuscript.
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Peer review information Nature Protocols thanks Andreas Möglich and the other, anonymous, reviewer(s) for their contribution to the peer review of this work.
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Supplementary information
Supplementary Information
Supplementary Notes 1 and 2, Supplementary Manual and Supplementary References
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Thomas, O.S., Hörner, M. & Weber, W. A graphical user interface to design high-throughput optogenetic experiments with the optoPlate-96. Nat Protoc 15, 2785–2787 (2020). https://doi.org/10.1038/s41596-020-0349-x
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DOI: https://doi.org/10.1038/s41596-020-0349-x
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