This is a preview of subscription content, access via your institution
Access options
Access Nature and 54 other Nature Portfolio journals
Get Nature+, our best-value online-access subscription
$29.99 / 30 days
cancel any time
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1038%2Fs41586-019-1623-3/MediaObjects/41586_2019_1623_Fig1_HTML.png)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1038%2Fs41586-019-1623-3/MediaObjects/41586_2019_1623_Fig2_HTML.png)
Data availability
Previously unpublished4 mtDNA sequence data from ES cell lines are available online (https://doi.org/10.5281/zenodo.3349761). Additional previously published data are available from the respective publications2,3. Further supporting sequence data from ref. 3 were provided to us by one of the authors (D. Egli).
References
Greenfield, A. et al. Assisted reproductive technologies to prevent human mitochondrial disease transmission. Nat. Biotechnol. 35, 1059–1068 (2017).
Kang, E. et al. Mitochondrial replacement in human oocytes carrying pathogenic mitochondrial DNA mutations. Nature 540, 270–275 (2016).
Yamada, M. et al. Genetic drift can compromise mitochondrial replacement by nuclear transfer in human oocytes. Cell Stem Cell 18, 749–754 (2016).
Hyslop, L. A. et al. Towards clinical application of pronuclear transfer to prevent mitochondrial DNA disease. Nature 534, 383–386 (2016).
Mackey, D. A. et al. Primary pathogenic mtDNA mutations in multigeneration pedigrees with Leber hereditary optic neuropathy. Am. J. Hum. Genet. 59, 481–485 (1996).
Carelli, V. et al. Biochemical features of mtDNA 14484 (ND6/M64V) point mutation associated with Leber’s hereditary optic neuropathy. Ann. Neurol. 45, 320–328 (1999).
Yu-Wai-Man, P. & Chinnery, P. F. in GeneReviews (eds Adam, M. P. et al.) https://www.ncbi.nlm.nih.gov/books/NBK1174/ (Univ. of Washington, 2000).
Leber, T. Ueber hereditäre und congenital-angelegte Sehnervenleiden. Albr. von Graefes Arch. für Ophthalmol. 17, 249–291 (1871).
Wallace, D. C. et al. Mitochondrial DNA mutation associated with Leber’s hereditary optic neuropathy. Science 242, 1427–1430 (1988).
Wallace, D. C. & Lott, M. T. in Pharmacology of Mitochondria (eds Singh, H. & Sheu, S.-S.) 339–376 (Springer International, 2017).
Hudson, G. et al. Clinical expression of Leber hereditary optic neuropathy is affected by the mitochondrial DNA-haplogroup background. Am. J. Hum. Genet. 81, 228–233 (2007).
Meyerson, C., Van Stavern, G. & McClelland, C. Leber hereditary optic neuropathy: current perspectives. Clin. Ophthalmol. 9, 1165–1176 (2015).
Kang, E. et al. Author Correction: Mitochondrial replacement in human oocytes carrying pathogenic mitochondrial DNA mutations. Nature 567, E5–E9 (2019).
Agaronyan, K., Morozov, Y. I., Anikin, M. & Temiakov, D. Replication-transcription switch in human mitochondria. Science 347, 548–551 (2015).
Acknowledgements
Work in the laboratory of G.H. and M.H. is funded by Wellcome (203105/Z/16/Z). G.H. is a Newcastle University Research Fellow and receives funding from the Michael J. Fox Foundation (ID: 15643).
Author information
Authors and Affiliations
Contributions
G.H., Y.T. and M.H. performed the analysis. G.H. and M.H. wrote the manuscript with input from Y.T.
Corresponding authors
Ethics declarations
Competing interests
The authors declare no competing interests.
Additional information
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Extended data figures and tables
Supplementary information
Rights and permissions
About this article
Cite this article
Hudson, G., Takeda, Y. & Herbert, M. Reversion after replacement of mitochondrial DNA. Nature 574, E8–E11 (2019). https://doi.org/10.1038/s41586-019-1623-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/s41586-019-1623-3
This article is cited by
-
Spatiotemporal Distribution and Function of Mitochondria in Oocytes
Reproductive Sciences (2024)
-
Mitochondrial DNA editing with mitoARCUS
Nature Metabolism (2023)
-
Reduction of mtDNA heteroplasmy in mitochondrial replacement therapy by inducing forced mitophagy
Nature Biomedical Engineering (2022)
-
Generation of somatic mitochondrial DNA-replaced cells for mitochondrial dysfunction treatment
Scientific Reports (2021)
-
Extreme heterogeneity of human mitochondrial DNA from organelles to populations
Nature Reviews Genetics (2021)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.