Abstract
Optical clearing with high-refractive-index (high-n) reagents is essential for 3D tissue imaging. However, the current liquid-based clearing condition and dye environment suffer from solvent evaporation and photobleaching, causing difficulties in maintaining the tissue optical and fluorescent features. Here, using the Gladstone-Dale equation [(nā1)/density=constant] as a design concept, we develop a solid (solvent-free) high-n acrylamide-based copolymer to embed mouse and human tissues for clearing and imaging. In the solid state, the fluorescent dye-labeled tissue matrices are filled and packed with the high-n copolymer, minimizing scattering in in-depth imaging and dye fading. This transparent, liquid-free condition provides a friendly tissue and cellular environment to facilitate high/super-resolution 3D imaging, preservation, transfer, and sharing among laboratories to investigate the morphologies of interest in experimental and clinical conditions.
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Introduction
Paraffin embedding is routinely used in medical laboratories to solidify and preserve tissues (e.g., formalin-fixed surgical specimens) for classic microtome-based histology. The solid wax strongly scatters light; thus, the wax-tissue composite is opaque prior to microtome sectioning and dewaxing for staining and microscopy. Alternatively, in modern 3D histology, investigators immerse the whole-mount specimen in the high-refractive-index (high-n) solution to reduce scattering for 3D confocal or light-sheet microscopy1,2,3,4,5. The refractive index n matching between the solution and the tissue/cellular components (proteins, DNA, and lipids; nāā„ā1.46)6 facilitates photon penetration, thereby enabling the fluorescently labeled tissue structures, such as the neurovascular networks, to be visualized in a 3D space continuum7,8,9,10. However, as with any liquid solution, the solvent of the clearing solution gradually evaporates, leading to solute precipitation and/or changes in chemical and optical conditions. There are also safety concerns when toxic or corrosive solvents (e.g., benzyl alcohol/ benzyl benzoate, known as BABB) involved in the clearing and imaging processes evaporate or leak to the environment. In addition, in 3D microscopy, a liquid holder as well as specimen mounting to the holder is required to maintain the n matching condition (e.g., the sample chamber in light-sheet microscopy). These constraints, compared with the streamlined clinical 2D histology, largely limit the use of 3D microscopy as a standard tool in tissue analysis.
To relieve the constraints imposed by the clearing liquid, preparation of transparent tissues in a solid, liquid-free condition provides an attractive strategy to fundamentally change the current setting for 3D tissue imaging. In the solid state, an extra advantage is that the firm and stable environment hinders chemical reactions (e.g., photobleaching) by limiting the diffusional contact between the indicator dye and reactive species (e.g., oxygen)11,12, thereby improving the shelf life and photochemical stability of the fluorescently labeled specimens. The photostability is particularly important in high-resolution 3D imaging (e.g., super-resolution microscopy) due to the prolonged laser exposure time under the high-power objective13.
Interestingly, similar to a variety of hydrophobic and hydrophilic high-n clearing liquids14,15,16, different classes of high-n polymers (e.g., phosphorus-, sulfur-, and halogen-containing polymers) have been identified and fabricated for optical applications, such as the microlenses array in complementary metal oxide semiconductor (or CMOS) sensors17,18,19. In bioimaging applications, however, the solid high-n polymers have not been explored and tested for tissue embedding and polymerization. Particularly, in polymerization, the non-physiological reaction conditions, such as high temperature or hydrophobicity, could denature and/or bleach the fluorophores used in tissue staining, affecting the subsequent morphological characterization.
Among the tissue-friendly polymers, the low-density polyacrylamide gel has long been used in electrophoresis and recently in the CLARITY/hydrogel approach to support the lipid-extracted tissue in the clearing solution for 3D microscopy20,21,22. By adjusting the hydrogelās composition, the chemical environment of the tissue-hydrogel complex, such as the electrostatic interactions, can be engineered to create a favorable condition for tissue clearing23,24. At a higher density, the transparent polyacrylamide and acrylamide-based polymers have also been explored for ophthalmological applications25,26,27. The high-n acrylamide-based polymers, in particular, provide a unique opportunity for simultaneous tissue embedding and clearing at a solid state to replace the high-n liquid in 3D imaging. Importantly, because the synthesis of polyacrylamide can be controlled by ultraviolet radiation, the photo-polymerization can be directly performed on the microscope glass to integrate the processes of tissue embedding, clearing, and 3D imaging with the same polymerized tissue slide.
Here, using an efficient two-step approach, we establish a tissue clearing method via tissue embedding in the high-n copolymer of acrylamide and n-hydroxymethyl acrylamide (or A-ha copolymer) for 3D fluorescence imaging with minimal photobleaching.
Results
High-n acrylamide-co-n-hydroxymethyl acrylamide copolymer
The refractive index n can be considered as the factor by which the speed of light is reduced with respect to its vacuum value. The empirical Gladstone-Dale equation28, (nā1)/Ļā=āconstant, further suggests that the increase in refractive index of a material (or reduction in light speed in material) vs. vacuum (nā=ā1) is proportional to the density Ļ. In polymer science, the macromolecules formed via covalent bonding are generally denser (higher Ļ, and thus higher n) than the monomers in solution prior to polymerization. The bonding reaction (polymerization) thus provides an effective way to increase the density of an organic mixture, creating a high-n environment for optical imaging.
Using the Gladstone-Dale equation as a design concept, we synthesized the high-n poly(acrylamide-co-n-hydroxymethyl acrylamide), or A-ha copolymer, by first linearly increasing the n to 1.475āĀ±ā0.001 (twelve repeats) with incremental increase in the monomers in water (Fig.Ā 1a), and then a jump of n to 1.533āĀ±ā0.005 (twenty-four repeats) via photo-polymerization at 86.7% monomer mass fraction. This ultrahigh monomer-to-water mass ratio (6.5ā1, Supplementary MovieĀ 1) was achieved by using a ternary solution of acrylamide (35.8%) and n-hydroxymethyl acrylamide (50.9%) in water (molar ratio 1:1), which avoids precipitation encountered in a binary acrylamide-water solution at the same mass fraction. Once polymerized, the A-ha copolymer is rigid and has a density at 1.30āĀ±ā0.01 (g/cm3, 20 repeats), an 18.1% increase compared with that of the monomer solution.
In polymerization, because cross-linkers (e.g., bisacrylamide) were not added as a reactant, the A-ha copolymer is thermoplastic and requires monomers at ā„76.0% in mass fraction to form a rigid material (Fig.Ā 1b and Supplementary Fig.Ā 1, vs. soft polyacrylamide gel). Importantly, the rigid A-ha copolymer can be fabricated into different shapes for tissue clearing (i.e., increase in transmittance) and imaging applications (Fig.Ā 1cāk). To evaluate the optical property, Fig.Ā 2 and Supplementary Fig.Ā 2 (extended list) compare the refractive indexes of the high-n polymers (A-ha, sulfur-, phosphorus-, and halogen-containing polymers) with those of the cellular components (proteins, DNA, and lipids) and published clearing reagents. Interestingly, because proteins and DNA can also be considered as copolymers with sulfur or phosphorus atoms, their estimated refractive indexes (ā„1.54)29,30,31 are closer to the high-n polymers than to the majority of the clearing reagents.
Two-step tissue clearing and 3D imaging with A-ha copolymer
To prepare transparent tissues, we established a two-step approach to increase the transmittance of the specimen of interest (Fig.Ā 3aāg). First, we immersed the tissue (500-Āµm mouse kidney section) in the A-ha monomer solution (nā=ā1.48) for an hour to elevate the transmittance to 64.4%āĀ±ā9.0% (wavelength 640ānm) from 4.9%āĀ±ā4.4% in PBS (nā=ā1.33). This was followed by photo-polymerization to synthesize the A-ha copolymer and transformed the immersed tissue to become an embedded specimen. The new optical condition (nā=ā1.53) further increases the transmittance to 93.5%āĀ±ā5.0% (wavelength 640ānm) of the same specimen. Importantly, the second step of the clearing process is efficiently achieved within 30āminutes because the free radical photo-polymerization has fast kinetics. In the process, we observed that the size of kidney increases from 100% (PBS) to 115āĀ±ā1% (twelve repeats in A-ha monomer solution, 1st step) to 118āĀ±ā2% (twelve repeats in A-ha copolymer, 2nd step), indicating a mild tissue expansion caused by the immersion and embedding processes (Supplementary Fig.Ā 3).
The compatibility of A-ha copolymer with fluorescence imaging is shown in Fig.Ā 4aāj. At the organ level, the A-ha-cleared mouse liver with nuclear and neurovascular labeling is prominently seen under the confocal microscope. In particular, the panoramic 2D image (Fig.Ā 4cāe) and 3D projection (Fig.Ā 4f, g) detect and identify the hepatic nerve plexus following the portal vein and its bifurcation in extension. Furthermore, using the high-power objective, we specified the detailed hepatic nervous and endothelial structures. FigureĀ 4hāj and Supplementary MovieĀ 2 (in-depth recording) present the perivascular sympathetic innervation with subcellular-level resolution, confirming the compatibility of A-ha copolymer with high-resolution microscopy. Additional examples of 3D fluorescence imaging with A-ha copolymer are presented in Supplementary Fig.Ā 4 and Supplementary MovieĀ 3 and 4 (mouse brain and kidney).
Multimodal cm-to-subcellular human pancreas imaging with A-ha
In clinical tissue analysis, we used the human donor pancreas with low-grade duct lesions (pancreatic intraepithelial neoplasia or PanIN)32,33 to demonstrate the integration of A-ha-based 3D histology with the clinical 2D histology. In Fig.Ā 5aāj, a side-by-side display of the adjacent vibratome and microtome sections (the former in A-ha, the latter in paraffin before sectioning) is used to illustrate the matched pancreatic lesion environment in the stereomicroscopic, hematoxylin and eosin (H&E), and fluorescence images. Specifically, in the A-ha copolymer, the transparent vibratome section enables in-depth neurovascular imaging of human pancreas with paired S100B/glial and CD31/endothelial fluorescent labeling (Fig.Ā 5eāj), whereas in the microtome section, the gold-standard H&E stain specifies the duct lesion (Fig.Ā 5d).
Clinically, using the 2D H&E histology, prior research has established that the PanIN lesion is associated with parenchymal (acinar) atrophy and fibrosis34,35. Here, using the 3D images (Supplementary MovieĀ 5), we detect that the PanIN and peri-PanIN stroma are also associated with S100B+ glial/nerve bundles (Fig.Ā 5eāg, 5- to 50-Āµm-thick; Supplementary MovieĀ 6 shows peri-lesional ganglion with neurons enclosed by glia)8,36. In the same region, the atrophic parenchyma is linked to less prominent CD31+ endothelium vs. adjacent normal lobules (Fig.Ā 5hāj). These two featuresāpreserved and revealed in the A-ha copolymerāappear to be the signs of peri-lesional neural association and reduced vascular supply to the atrophic lobule. Comparable but drastic changes of pancreatic lobules, including loss of acini, fibrosis, neural invasion, and lack of vascular supply, have been previously reported in pancreatic cancer37,38,39,40.
In addition to the change in the neurovascular association, we also identified the endocrine pancreas remodeling around the exocrine duct lesion. This phenomenon is highlighted by the detection of duct-islet cell clusters (Fig.Ā 5kāo and Supplementary MovieĀ 7)33,41. In the A-ha copolymer, the cluster is well preserved and can be identified with glucagon (Ī±-cell), insulin (Ī²-cell), and cytokeratin 7 (CK7, duct cell) staining. When enlarged, the endocrine Ī±- and Ī²-cells were seen juxtaposed with the exocrine duct cells (Fig.Ā 5l), a sharp contrast to the discrete units of islets and ducts in the normal lobules. Furthermore, we used the Airyscan detector array microscopy (Carl Zeiss, super-resolution mode)42 to enhance the resolving power of cellular imaging in the A-ha copolymer. In the duct-islet cell cluster, the glucagon+ and insulin+ vesicles were identified in the Ī±- and Ī²-cells, respectively, adjacent to the CK7+ duct cells (Fig.Ā 5māo). Technically, this series of human pancreas imagingāfrom duct lesion to islet cells to vesiclesādemonstrates the multimodal, multidimensional, and multiscale approaches of imaging with the A-ha copolymer.
Antifade and super-resolution 3D human pancreas imaging
One fundamental difference of fluorescence imaging in the high-n A-ha copolymer vs. high-n clearing solution is photochemical stability. This distinct property can be visualized by time-series confocal imaging of the fluorescence derived from direct immunohistochemistry. We used the human pancreas vasculature (due to its abundance, e.g., Fig.Ā 5h) labeled with the sensitive Alexa Fluor (AF) ā647-conjugated anti-CD31 primary antibody to demonstrate this phenomenon.
In Fig.Ā 6, we compare the decreases in AF-647 signals in time-series confocal imaging while the specimens were embedded in the A-ha copolymer, immersed in the aqueous Ce3Dā¢ solution (nā=ā1.5, Biolegend; tissue clearing control)43, or immersed in the organic solvent-based CytoVistaā¢ clearing solution (nā=ā1.5, ThermoFisher; tissue clearing control)44,45. The less sensitive indirect immunohistochemistry (anti-S100B labeled with multiple AF-546-secondary antibodies, e.g., Fig.Ā 5e) and chemical stain of nuclei (DAPI) were used as the positive controls to monitor the fluorescence signals. Importantly, after 500 frames were taken from the solid and liquid clearing conditions (90-min. continuous imaging of a 320Ć320-Āµm region), the mean AF-647 fluorescence in A-ha decreased by 9āĀ±ā2% (Fig.Ā 6aāc), which was a marked difference compared with the 95Ā±4% and 55Ā±7% decreases of the same indicator dye in the Ce3Dā¢ and CytoVistaā¢, respectively (Fig.Ā 6dāf, gāi; six repeats; pā<ā0.001). The antifade feature of fluorescence imaging with A-ha copolymer is presented in Supplementary MovieĀ 8. The comparison of AF-647 stability when embedded in A-ha vs. immersed in liquids applied in the iDISCO (dibenzyl ether)46, CLARITY (FocusClear)20, and CUBIC (sucrose/urea/triethanolamine solution)3 -based clearing methods is presented in Supplementary Fig.Ā 5aāl. Supplementary MovieĀ 9 shows the side-by-side and frame-by-frame comparison of the four conditions. The intrinsic stability of nuclear dyesāDAPI (positive control), Hoechst, and propidium iodideāin A-ha and Ce3D is presented in Supplementary Fig.Ā 5m, n.
Because the A-ha copolymer enables both antifade and 3D fluorescence imaging, we next applied multimodal imaging (as illustrated in Fig.Ā 5) with in-depth Airyscan imaging (super-resolution mode) to resolve the unique 3D neurovascular features of human pancreas, which otherwise cannot be effectively achieved with the clinical 2D histology or 3D imaging in the clearing liquid. FigureĀ 7 is an example of this approach, featuring the human pancreas lobule-to-nucleolus magnification, starting from the gross view of vascular pathway (Fig.Ā 7a, b; CD31+) and ending at the dimly stained neuronal nucleus in the intrapancreatic ganglion (Fig.Ā 7cāg; also see Supplementary MovieĀ 10ā12). Overall, the antifade and solvent-free conditions of A-ha create a 3D imaging platform that mimics the chemical (stable) and optical (transparent) environments of clinical 2D histology for high/super-resolution 3D imaging on the standard microscope slide (Supplementary Fig.Ā 6).
Discussion
High-resolution 3D fluorescence imaging prefers stable chemical and optical conditions to resolve the dye-labeled tissue and cellular structures in health and disease. Although major advances in the machine (e.g., detector and illumination strategy), indicator dye (e.g., quantum dot), and suppression of scattering (tissue clearing) have improved the resolving power in in-depth imaging, the intrinsic volatility and molecular diffusion of liquids limit the current approach and design for 3D histology. Here, using the high-n A-ha copolymer, we created a transparent environment for 3D tissue imaging in a solvent-free condition. The tissue embedding was made possible by photo-polymerization at room temperature in an acrylamide-based solution, minimizing concerns of tissue damage and artifacts created in a harsh condition of polymer synthesis.
Importantly, using the experimental and clinical specimens, we established the organ-to-nucleus and panoramic-to-super-resolution imaging, respectively, confirming the intact macro- and micro-structures of the embedded tissues in the A-ha copolymer. Particularly, using the human pancreas, we demonstrated the matched duct lesion microenvironment in the stereomicroscopic, fluorescence, and gold-standard H&E images, highlighting the 3D/2D multimodal imaging with the A-ha copolymer in a clinically related setting. It should be noted that because the standard organic dyes (DAPI and Alexa Fluor) are stable in the solid A-ha copolymer, but not the fluorescent proteins, which evolve in an aqueous environment, our work is designed for 3D histology following standard (direct or indirect) immunohistochemistry. In immunohistochemistry, a stable dye environment benefits the use of high/super-resolution imaging by minimizing photobleaching in procedures that require concentrated laser excitation and/or prolonged imaging time to record in-depth 3D signals (e.g., Fig.Ā 7 and Supplementary MovieĀ 10ā12).
In 3D signal detection, because false-negative results are frequently encountered in fluorescent immunohistochemistry, the antifade vascular (anti-CD31) and nuclear (DAPI) labeling developed in this research provides the important positive controls for clinical tissue examinationādue to their abundanceāto monitor the penetration of antibody and indicator dye in 3D labeling. In the imaging facility core, the antifade controls also offer a suitable chemical environment to compare different super-resolution methods (e.g., Airyscan vs. stimulated emission depletion, or STED, microscopy) via a standard 3D specimen (e.g., Supplementary Fig.Ā 6c).
From the logistic perspective, because the indicator dyes are stable in the solid A-ha copolymer, the improved shelf life allows transfer of 3D specimens among laboratories for off-site imaging (e.g., at microscope center) and/or tissue preservation and archiving, alleviating the constraints of facility and time in 3D tissue analysis. In particular, sharing of the antifade 3D tissues benefits resource and information exchanges in the pandemic/post-pandemic era, in which international interactions and in-person visits are significantly reduced. In terms of safety in tissue clearing, the replacement of organic solvents with solid polymers in 3D imaging protects staffs as well as machines in the microscope room, in which solvent evaporation or leakage is a potential concern compared with the standard 2D imaging. Regarding safety in polymer synthesis, we would like to stress that the monomers of A-ha copolymerāacrylamide and n-hydroxymethyl acrylamideāare toxins when in solution (especially acrylamide), so care must be taken to avoid direct contact with the monomer solution. Once polymerized, similar to the polyacrylamide gel used in electrophoresis, the A-ha copolymer is chemically stable (long shelf life) with strong mechanical strength (provided by ultrahigh monomer content, Fig.Ā 1a) to protect the cleared 3D specimen in transport and imaging.
In conclusion, before this research, 3D tissue imaging has been limited by solvent and dye instability in a liquid environment. Using the Gladstone-Dale equation as a design concept, we develop a solid (solvent-free) high-n A-ha copolymer to embed mouse and human tissues for clearing and antifade imaging. This solid microscopic environment offers a friendly and versatile platform that integrates with standard fluorescent dye labeling, clinical 2D histology, and 3D super-resolution imaging for investigation of the cellular structures and neurovascular networks in health and disease.
Methods
Ethical statement
Collection and use of human tissues were approved by the Institutional Review Board of National Taiwan University Hospital (NTUH-REC No.: 202107126RINC). Human pancreases were obtained from deceased organ donors whose pancreases were declined for transplantation but whose next of kin had given written consent for use in research. The Institutional Animal Care and Use Committees at National Tsing Hua University approved all procedures with mice (Approval No.: 110037).
Synthesis of high-n A-ha copolymer
Monomers acrylamide and n-hydroxymethyl acrylamide were purchased from Sigma-Aldrich (St. Louis, MO, USA; A8887) and TCI (Tokyo Chemical Industry, Tokyo, Japan; M0574), respectively (caution: both monomers must be handled in a chemical fume hood). Irgacure 2959 (Sigma-Aldrich, 410896) was used as the photoinitiator for the UV-induced photo-polymerization (Supplementary Fig.Ā 6). A-ha monomer solution was prepared on a pre-warmed plate (60ā65āĀ°C) by adding 5āg of double-distilled water with 0.3% Irgacure 2959 (0.015āg) to a mixture of 13.48āg of acrylamide and 19.1āg of n-hydroxymethyl acrylamide (Supplementary MovieĀ 1). The solution was stable at room temperature and was sealed in dark prior to reaction. Before and after UV irradiation (30āmin), the refractive indexes of A-ha monomer solution and copolymer were measured by a liquid refractometer (Rocker Scientific, Kaohsiung, Taiwan; model: BR92D/BR95D; sucrose, acrylamide, and n-hydroxymethyl acrylamide solutions were used as controls) and a gem refractometer (Fable Gem Refractometer FGR-002A, Guangdong, China), respectively. The photo-polymerization was confirmed by the weak/absent peak of alkenes at 1683ācmā1 of A-ha copolymerās FT-IR spectrum (Nicolet iS50, ThermoFisher, Waltham, MA, USA) vs. the spectra of the two monomers (Supplementary Fig.Ā 6d).
Animal and human tissues
Kidneys and livers harvested from 8 to 12-week wild-type male C57BL/6 mice (National Laboratory Animal Center, Taipei, Taiwan) were used to develop 3D histology with A-ha copolymer. Mice were housed in a controlled environment at 22āĀ°C, 50% humidity, and 12-h light-dark cycles. Human pancreases obtained from deceased organ donors were used to evaluate the compatibility of A-ha copolymer with clinical tissue embedding and imaging. Perfused pancreases from two donors (male, no history of diabetes or pancreatic diseases, aged 40-55, with BMI 20.4-23.6) with normal HbA1c, amylase, and lipase levels were used to characterize the pancreatic lobular environment in health and pancreatic intraepithelial neoplasia (PanIN). The two donor pancreases were grossly normal, but low-grade PanIN lesions were detected by organ-wide examination of vibratome sections via stereomicroscopy; the PanIN lesions were subsequently confirmed by H&E histology41.
Tissue preparation and labeling
Blood vessels of mouse liver, brain, and kidney were labeled with cardiac perfusion of lectin-Alexa Fluor 488 conjugates (ThermoFisher) followed by 4% paraformaldehyde perfusion fixation47,48. Afterward, the organs were harvested and post-fixed in 4% paraformaldehyde solution for 30āminutes at 15āĀ°C. The human donor pancreas was first cut to separate the head, body, and tail of the organ and then into strips (~1.5ācm in width)41. Afterward, the tissue was fixed in formaldehyde 10% (w/v; Macron Fine Chemicals, Centre Valley, PA, USA, H121-08) for two days and then washed in PBS for four days at 4āĀ°C. The fixed mouse and human specimens were later sectioned to 500āĀµm (or 350āĀµm in Fig.Ā 5) in thickness by vibratome and transferred to 0.1% paraformaldehyde and 0.25% formaldehyde, respectively, for preservation at 4āĀ°C.
Prior to immunolabeling, the samples were dehydrated by rinsing in methanol/water series (25%ā50%ā75%ā100%) and then immersed in 100% methanol for 5āh. Afterward, the specimens were bleached with 5% hydrogen peroxide (Sigma-Aldrich, 31642) in 100% methanol overnight at 4āĀ°C. The rehydration process was performed by downgrading serials of methanol/water (25%ā50%ā75%ā100%-PBS). The rehydrated specimens were then immersed in 2% Triton X-100 solution at 15āĀ°C overnight for permeabilization.
Seven different primary antibodies were used alone or in combination to immunolabel the tissues following the protocol outlined below. The antibodies used were rabbit anti-tyrosine hydroxylase (TH, sympathetic marker; AB152, Sigma-Aldrich), rabbit anti-S100B (glial marker; ab52642, Abcam, Cambridge, MA, USA), AF-647-conjugated mouse anti-CD31 (endothelial marker, ab215912, Abcam), mouse anti-glucagon (islet Ī±-cell marker; ab10988, Abcam), AF-488-conjugated mouse anti-insulin (islet Ī²-cell marker; sc-8033_AF488, Santa Cruz Biotechnology, Dallas, TX, USA), rabbit anti-CK7 (epithelial marker; ab68459, Abcam), and AF-647-conjugated rabbit anti-PGP9.5 (neuronal marker; ab196173, Abcam) antibodies.
Before applying the antibodies, the permeabilized tissue sections were rinsed in PBS. This was followed by a blocking step, incubating the tissue with the blocking buffer (2% Triton X-100, 10% normal goat serum, and 0.02% sodium azide in PBS) for 2āh at 15āĀ°C. The primary antibody was then diluted in the dilution buffer (1:100, 0.25% Triton X-100, 1% normal goat serum, and 0.02% sodium azide in PBS) to replace the blocking buffer and incubated for two days at 15āĀ°C. Negative staining controls were prepared by omitting the primary antibody in the buffer.
Alexa Fluor 488, 546, and 647-conjugated secondary antibodies (raised in goat; 1:200, ThermoFisher) were used in combination to reveal the immunostained structures. DAPI (Sigma-Aldrich, D9542) staining was performed to reveal the nuclei. The fluorescently labeled mouse and human specimens were immersed in 4% paraformaldehyde and 10% formaldehyde, respectively, at 4āĀ°C overnight for post-fixation prior to tissue clearing and imaging. Supplementary TableĀ 1 summarizes the immunostaining reagents/dilutions used in the illustrations.
A-ha-based tissue clearing and 3D microscopy
The vibratome section of mouse or human tissue was first immersed in one well of a 6-well plate with 3āml of the A-ha monomer solution and placed on a platform rotator for 15āminutes and subsequently transferred to a new well with 3-ml A-ha monomer solution for another 45āminutes. Afterward, a 500-Ī¼m-iSpacer (SunJin Lab, Hsinchu, Taiwan) was placed on a coverslip, which was followed by adding fresh A-ha monomer solution (~360āĪ¼l) to the spacer compartment and then transferring the tissue from the six-well plate to the spacer. After adding another coverslip on top, the sample was ready for photo-polymerization (Supplementary Fig.Ā 6b). In the clearing kinetics test (Fig.Ā 3aāg), the 500-Ī¼m mouse kidney sections (without labeling) were used to measure the tissue transmittance (Rainbow Light Technology, Transmittance Measuring Equipment TSM-01, Taoyuan, Taiwan) vs. time of UV irradiation (lamp: Philips, TUV PL-L 18W/4P 1CT/25, emission peak at 253.7ānm, irradiance: 9.6āmW/cm2, measured by Accumulated UV Meter UIT-250, Ushio America, Cypress, CA, USA; Supplementary Fig.Ā 6a, b). Thirty minutes of UV irradiation were used as the standard (unless otherwise indicated) to prepare transparent mouse and human specimens for 3D confocal microscopy (Zeiss LSM 800, Carl Zeiss, Jena, Germany; note: A-ha copolymer absorbs water; avoid direct water-A-ha copolymer contact in microscopy) and super-resolution imaging (Zeiss Airyscan, a 32-detector array). The Avizo 6.2 image reconstruction software (VSG, Burlington, MA, USA), Zen software (blue and black editions, Carl Zeiss), and LSM 510 software (Carl Zeiss) were used for noise reduction, projection, and analysis of the fluorescence images. Fluorescence signals in the figures and supplementary movies were pseudo-colored. Supplementary TableĀ 2 summarizes the color codes for different markers in the illustrations.
Airyscan super-resolution imaging of human pancreas
Prior to Airyscan, standard confocal imaging with x10 (C-Apochromat Ć10/0.45āW, Carl Zeiss), Ć25 (LD LCI Plan-Apochromat Ć25/0.8 Imm Corr DIC, Carl Zeiss), and/or Ć40 (LD C-Apochromat Ć40/1.1āW, Carl Zeiss) objectives were performed to evaluate the lobular environment and to identify the area of interest (PanIN in Fig.Ā 5 and ganglion in Fig.Ā 7). In Airyscan, super-resolution imaging with Ć40 (Plan-Apochromat Ć40/1.3 Oil DIC) and Ć63 (Plan-Apochromat Ć63/1.4 Oil DIC) objectives were sequentially applied (laser power, 3ā5%; master gain, 650ā780āV; pixel dwell time, Ć40 objective: 2.06āĀµs, Ć63 objective: 3.82āĀµs; additional parameters are listed in Supplementary Fig.Ā 7). The first round (Ć40) was to integrate the super-resolution images with the panoramic view of pancreas; the second round (Ć63) offers detailed examination of the subcellular structures. The multiple rounds of fluorescence imaging were made possible by the antifade feature of A-ha-based fluorescence imaging (Fig.Ā 6aāc).
Antifade test of fluorescence microscopy
Human pancreas vasculature (500-Āµm specimen) labeled with Alexa Fluor (AF) ā647-conjugated anti-CD31 primary antibody (direct immunohistochemistry, sensitive to photobleaching) was used in Zeiss LSM 800 time-series imaging to evaluate the decrease in fluorescence (Histogram function of Zen software, Carl Zeiss) against cycles of excitation and emission (frames taken). In parallel, the less sensitive DAPI labeling of nuclei (chemical stain) and indirect immunohistochemistry (anti-S100B labeled with multiple AF-546-secondary antibodies) were used as the labeling controls to monitor the fluorescence signals and imaging process (Ć40 objective, LD C-Apochromat Ć40/1.1āW, Carl Zeiss; ~30āĀµm under tissue surface; 500 cycles; 90āmin). The triple-labeled specimens were embedded in A-ha or immersed in the clearing reagent Ce3D (aqueous solution, liquid control; BioLegend, San Diego, CA, USA; 427702) or CytoVista (organic solvent, liquid control; ThermoFisher, V11300) to perform the antifade test.
Multimodal A-ha/3D and clinical/2D pancreas histology
Vibratome sections of human pancreas (350āĀµm in thickness; Fig.Ā 5a, b) were examined with stereomicroscopy to detect the potential duct lesion41. Afterward, the area of interest was examined by clinical 2D histology (H&E stain of microtome section, gold standard) to confirm the PanIN lesion (Fig.Ā 5d). Once confirmed, fluorescence labeling of the adjacent vibratome sections (Fig.Ā 5eāj,Ā kāo) was performed to reveal the neurovascular networks and duct-islet cell clusters in the same microenvironment. Both the standard confocal and Airyscan microscopy (super-resolution mode, Carl Zeiss) were used to characterize the peri-lesional microenvironment.
Statistics and reproducibility
The quantitative values are presented as means with standard deviation (except Fig.Ā 1a). Statistical differences were determined by the two-sided unpaired Studentās t test. Differences between groups were considered statistically significant when pā<ā0.05. No statistical method was used to predetermine sample size. No data were excluded from the analyses. The experiments were not randomized. The investigators were not blinded to allocation during experiments and outcome assessment.
Reporting summary
Further information on research design is available in theĀ Nature Portfolio Reporting Summary linked to this article.
Data availability
Considering the large size of 3D confocal and Airyscan super-resolution image files, the raw datasets are available from the corresponding author S.C.T. on request. Requests will be answered within 3 weeks to arrange file transfer.Ā Source data are provided with this paper.
References
Fu, Y. Y. et al. Microtome-free 3-dimensional confocal imaging method for visualization of mouse intestine with subcellular-level resolution. Gastroenterology 137, 453ā465 (2009).
Erturk, A. & Bradke, F. High-resolution imaging of entire organs by 3-dimensional imaging of solvent cleared organs (3DISCO). Exp. Neurol. 242, 57ā64 (2013).
Susaki, E. A. et al. Advanced CUBIC protocols for whole-brain and whole-body clearing and imaging. Nat. Protoc. 10, 1709ā1727 (2015).
Silvestri, L., Costantini, I., Sacconi, L. & Pavone, F. S. Clearing of fixed tissue: a review from a microscopistās perspective. J. Biomed. Opt. 21, 081205 (2016).
Azaripour, A. et al. A survey of clearing techniques for 3D imaging of tissues with special reference to connective tissue. Prog. Histochem. Cytochem. 51, 9ā23 (2016).
Tainaka, K., Kuno, A., Kubota, S. I., Murakami, T. & Ueda, H. R. Chemical Principles in tissue clearing and staining protocols for whole-body cell profiling. Annu. Rev. Cell Dev. Biol. 32, 713ā741 (2016).
Campbell-Thompson, M. & Tang, S. C. Pancreas optical clearing and 3-D microscopy in health and diabetes. Front. Endocrinol. 12, 644826 (2021).
Tang, S. C. et al. Human pancreatic neuro-insular network in health and fatty infiltration. Diabetologia 61, 168ā181 (2018).
Lugo-Hernandez, E. et al. 3D visualization and quantification of microvessels in the whole ischemic mouse brain using solvent-based clearing and light sheet microscopy. J. Cereb. Blood Flow. Metab. 37, 3355ā3367 (2017).
Lagerweij, T. et al. Optical clearing and fluorescence deep-tissue imaging for 3D quantitative analysis of the brain tumor microenvironment. Angiogenesis 20, 533ā546 (2017).
Demchenko, A. P. Photobleaching of organic fluorophores: quantitative characterization, mechanisms, protection. Methods Appl. Fluoresc. 8, 022001 (2020).
Bernas, T., Zarebski, M., Dobrucki, J. W. & Cook, P. R. Minimizing photobleaching during confocal microscopy of fluorescent probes bound to chromatin: role of anoxia and photon flux. J. Microsc. 215, 281ā296 (2004).
Kwon, J., Elgawish, M. S. & Shim, S. H. Bleaching-resistant super-resolution fluorescence microscopy. Adv. Sci. 9, e2101817 (2022).
Richardson, D. S. & Lichtman, J. W. Clarifying tissue clearing. Cell 162, 246ā257 (2015).
Orlich, M. & Kiefer, F. A qualitative comparison of ten tissue clearing techniques. Histol. Histopathol. 33, 181ā199 (2018).
Ueda, H. R. et al. Tissue clearing and its applications in neuroscience. Nat. Rev. Neurosci. 21, 61ā79 (2020).
Higashihara, T. & Ueda, M. Recent progress in high refractive index polymers. Macromolecules 48, 1915ā1929 (2015).
Childs, A. et al. Fabricating customized hydrogel contact lens. Sci. Rep. 6, 34905 (2016).
Giusto, P. et al. Shine bright like a diamond: new light on an old polymeric semiconductor. Adv. Mater. 32, e1908140 (2020).
Chung, K. et al. Structural and molecular interrogation of intact biological systems. Nature 497, 332ā337 (2013).
Chung, K. & Deisseroth, K. CLARITY for mapping the nervous system. Nat. Methods 10, 508ā513 (2013).
Jensen, K. H. R. & Berg, R. W. Advances and perspectives in tissue clearing using CLARITY. J. Chem. Neuroanat. 86, 19ā34 (2017).
Ono, Y., Nakase, I., Matsumoto, A. & Kojima, C. Rapid optical tissue clearing using poly(acrylamide-co-styrenesulfonate) hydrogels for three-dimensional imaging. J. Biomed. Mater. Res. B Appl. Biomater. 107, 2297ā2304 (2019).
Kojima, C. et al. Application of zwitterionic polymer hydrogels to optical tissue clearing for 3D fluorescence imaging. Macromol. Biosci. 21, e2100170 (2021).
Yan, C., Kramer, P. L., Yuan, R. & Fayer, M. D. Water dynamics in polyacrylamide hydrogels. J. Am. Chem. Soc. 140, 9466ā9477 (2018).
Teng, W. et al. A tough, precision-porous hydrogel scaffold: ophthalmologic applications. Biomaterials 35, 8916ā8926 (2014).
Zhou, C. et al. High water content hydrogel with super high refractive index. Macromol. Biosci. 13, 1485ā1491 (2013).
Gladstone, J. H. & Dale, T. P. Researches on the refraction, dispersion, and sensitiveness of liquids. Philos. Trans. R. Soc. 153, 317ā343 (1863).
Bashkatov, A. N. et al. Measurement of tissue optical properties in the context of tissue optical clearing. J. Biomed. Opt. 23, 1ā31 (2018).
Tuchin, V. V. Tissue optics and photonics: light-tissue interaction. J. Biomed. Photon. Eng. 1, 98ā134 (2015).
Inagaki, T., Hamm, R. N., Arakawa, E. T. & Painter, L. R. Optical and dielectric properties of DNA in extreme ultraviolet. J. Chem. Phys. 61, 4246ā4250 (1974).
Basturk, O. et al. A revised classification system and recommendations from the baltimore consensus meeting for neoplastic precursor lesions in the pancreas. Am. J. Surg. Pathol. 39, 1730ā1741 (2015).
Tien, Y. W. et al. Local islet remodelling associated with duct lesion-islet complex in adult human pancreas. Diabetologia 64, 2266ā2278 (2021).
Andea, A., Sarkar, F. & Adsay, V. N. Clinicopathological correlates of pancreatic intraepithelial neoplasia: a comparative analysis of 82 cases with and 152 cases without pancreatic ductal adenocarcinoma. Mod. Pathol. 16, 996ā1006 (2003).
Detlefsen, S., Sipos, B., Feyerabend, B. & Kloppel, G. Pancreatic fibrosis associated with age and ductal papillary hyperplasia. Virchows Arch. 447, 800ā805 (2005).
Chien, H. J. et al. Human pancreatic afferent and efferent nerves: mapping and 3-D illustration of exocrine, endocrine, and adipose innervation. Am. J. Physiol. Gastrointest. Liver Physiol. 317, G694āG706 (2019).
Demir, I. E., Friess, H. & Ceyhan, G. O. Nerve-cancer interactions in the stromal biology of pancreatic cancer. Front. Physiol. 3, 97 (2012).
Roger, E. et al. Schwann cells support oncogenic potential of pancreatic cancer cells through TGFbeta signaling. Cell Death Dis. 10, 886 (2019).
Oberstein, P. E. & Olive, K. P. Pancreatic cancer: why is it so hard to treat? Ther. Adv. Gastroenterol. 6, 321ā337 (2013).
Komar, G. et al. Decreased blood flow with increased metabolic activity: a novel sign of pancreatic tumor aggressiveness. Clin. Cancer Res. 15, 5511ā5517 (2009).
Chung, M. H. et al. Multimodal 3-D/2-D human islet and duct imaging in exocrine and endocrine lesion environment: associated pancreas tissue remodeling. Am. J. Physiol. Endocrinol. Metab. 323, E354āE365 (2022).
Huff, J. The Airyscan detector from ZEISS: confocal imaging with improved signal-to-noise ratio and super-resolution. Nat. Methods 12, 1205 (2015).
Li, W., Germain, R. N. & Gerner, M. Y. Multiplex, quantitative cellular analysis in large tissue volumes with clearing-enhanced 3D microscopy (Ce3D). Proc. Natl Acad. Sci. USA 114, E7321āE7330 (2017).
Wang, S., Matsumoto, K., Lish, S. R., Cartagena-Rivera, A. X. & Yamada, K. M. Budding epithelial morphogenesis driven by cell-matrix versus cell-cell adhesion. Cell 184, 3702ā3716.e3730 (2021).
Nurnberg, E. et al. Routine optical clearing of 3D-cell cultures: simplicity forward. Front. Mol. Biosci. 7, 20 (2020).
Renier, N. et al. iDISCO: a simple, rapid method to immunolabel large tissue samples for volume imaging. Cell 159, 896ā910 (2014).
Juang, J. H., Peng, S. J., Kuo, C. H. & Tang, S. C. Three-dimensional islet graft histology: panoramic imaging of neural plasticity in sympathetic reinnervation of transplanted islets under the kidney capsule. Am. J. Physiol. Endocrinol. Metab. 306, E559āE570 (2014).
Chen, C. C. et al. Heterogeneity and neurovascular integration of intraportally transplanted islets revealed by 3-D mouse liver histology. Am. J. Physiol. Endocrinol. Metab. 320, E1007āE1019 (2021).
Fujiya, A. et al. The role of S100B in the interaction between adipocytes and macrophages. Obesity 22, 371ā379 (2014).
Acknowledgements
This work was supported in part by grants from Taiwan Ministry of Science and Technology (MOST 111-2740-B-001-005) to C.N.S. and S.C.T., MOST 111-2314-B-007-005-MY2 to S.C.T., Taiwan Academia Sinica (AS-107-TP-L15) to C.N.S., Y.W.T., and S.C.T., Taiwan National Health Research Institutes (NHRI-EX111-10922EI and NHRI-EX112-11225EI) to Y.W.T. and S.C.T., and National Tsing Hua University, Taiwan (intramural 110F7MAKE1) to S.C.T. The authors are grateful for the support from the confocal imaging core in National Tsing Hua University, Taiwan, which is sponsored by the Ministry of Science and Technology (MOST 110-2731-M-007-001). We thank Dr. S.-Ja Tseng at the Graduate Institute of Oncology, National Taiwan University and Dr. Ho-Hsiu Chou at the Department of Chemical Engineering, National Tsing Hua University, Taiwan, for FT-IR analysis of A-ha copolymer.
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All authors contributed to the study concept and design; F.T.H. and H.J.C. contributed to A-ha copolymer synthesis and characterization; Y.W.T., H.P.C., C.Y.L., C.C.C., and Y.M.J. contributed to human pancreas acquisition and preparation for 3D imaging; F.T.H., H.J.C., T.H.H., and S.J.P., contributed to mouse tissue preparation; F.T.H., H.J.C., Y.H.C., S.J.P., M.H.C., T.H.H., L.W. L., C.N.S., and S.C.T. contributed to 3D tissue imaging and image presentation; and S.C.T. contributed to drafting of the manuscript. C.N.S., Y.W.T., and S.C.T. obtained funding. All authors contributed to data analysis and interpretation of data, revised the manuscript critically for intellectual content, and approved the final version of the manuscript. S.C.T. is the guarantor of this work and, as such, had full access to all the data in the study and takes responsibility for the integrity of the data and the accuracy of the data analysis.
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Hsiao, FT., Chien, HJ., Chou, YH. et al. Transparent tissue in solid state for solvent-free and antifade 3D imaging. Nat Commun 14, 3395 (2023). https://doi.org/10.1038/s41467-023-39082-4
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DOI: https://doi.org/10.1038/s41467-023-39082-4
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