Abstract
There are rich and vast genetic resources of indigenous pig breeds in the world. Currently, great attention is paid to either crossbreeding or conservation of these indigenous pig breeds, and insufficient attention is paid to the combination of conservation and breeding along with their long-term effects on genetic diversity. Therefore, the objective of this study is to compare the long-term effects of using conventional conservation and optimal contribution selection methods on genetic diversity and genetic gain. A total of 11 different methods including conventional conservation and optimal contribution selection methods were investigated using stochastic simulations. The long-term effects of using these methods were evaluated in terms of genetic diversity metrices such as expected heterozygosity (He) and the rate of genetic gain. The results indicated that the rates of true inbreeding in these conventional conservation methods were maintained at around 0.01. The optimal contribution selection methods based either on the pedigree (POCS) or genome (GOCS) information showed more genetic gain than conventional methods, and POCS achieved the largest genetic gain. Furthermore, the effect of using GOCS methods on most of the genetic diversity metrics was slightly better than the conventional conservation methods when the rate of true inbreeding was the same, but this also required more sires used in OCS methods. According to the rate of true inbreeding, there was no significant difference among these conventional methods. In conclusion, there is no significant difference in different ways of selecting sows on inbreeding when we use different conventional conservation methods. Compared with conventional methods, POCS method could achieve the most genetic gain. However, GOCS methods can not only achieve higher genetic gain, but also maintain a relatively high level of genetic diversity. Therefore, GOCS is a better choice if we want to combine conservation and breeding in actual production in the conservation farms.
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Introduction
The genetic diversity of livestock is essential to improve productivity and respond to challenges including food security and climate change mitigation in the future (Groeneveld et al. 2010). Pigs are one of the most common domestic animals, and more than one-third of indigenous pig breeds in the world are in China (Ai et al. 2015). These indigenous pig breeds generally have high fertility, good meat quality and high tolerance to harsh environmental conditions (Yang 2013). However, pig industries currently pay more attention to crossbreeding for indigenous pig breeds, and insufficient attention to the combination of conservation and genetic improvement. To improve the production and economic value of breeding stocks, indigenous pig breeds usually cross with the foreign breeds which have high production performance. Thus, gene flow usually only occurs from breeds with superior economic characteristics to indigenous pig breeds (Wellmann et al. 2012). Furthermore, due to the epidemic of diseases such as African swine fever, a lot of precious indigenous breeds are on the verge of extinction. The herds of indigenous pig breeds have reduced greatly during this period. To protect these indigenous pig breeds in China, the Chinese government has established national-level breeding farms for most indigenous pig breeds, and a large number of breeding funds are used to protect these unique indigenous pig breeds every year (Zhao et al. 2021).
In these national-level conservation farms, our goal is to maintain each breed’s genetic materials and control the rate of inbreeding as much as possible. Inbreeding is an important reason for the loss of genetic variation, and the rate of inbreeding mainly depends on the effective population size (Pekkala et al. 2014). In order to reduce the impact of inbreeding on the loss of population genetic variation, the increase in the inbreeding coefficient of each generation in the conservation population is recommended to be controlled at 1–4%, and the conservation population needs to have at least 12–25 sires and 100–250 dams (Smith 1984). These guides are commonly used in conservation farms. Simultaneously, to keep the genetic diversity and control rate of inbreeding, conservation farms attempt to keep the same number of offspring for each family. However, current conservation methods do not combine conservation of genetic resources and genetic improvement of production performance. In conservation populations, it is also very important to properly select the dominant traits of each local pig breed, which will help to further consolidate the advantages of the breed and maintain the uniqueness of each breed. From a long-term sustainable perspective, how to combine conservation and selective breeding in the conservation field is a crucial issue. Great attention should be paid to genetic improvement of important economic traits while maintaining the overall genetic diversity of local breeds to meet pig industry’s sustainable development and even other livestock industries. Therefore, we need to re-examine our current conservation strategy. The new conservation strategy should take into account at least two principals at the same time. One of the principles is to keep genetic diversity as high as possible, and the other is to obtain genetic progress of some essential economic traits as large as possible.
Optimal contribution selection (OCS) is an effective selection method that balances inbreeding and genetic gain (Dagnachew and Meuwissen 2016; Gomez-Romano et al. 2016). It maximizes rates of predicted genetic gain while controlling inbreeding at given rates by optimizing the genetic contribution of each selection candidate to the next generation (Grundy et al. 1998; Hayes et al. 2013; Wang et al. 2017). Optimal contribution selection can be based either on pedigree information (POCS) or genome information (GOCS). Several previous studies have investigated the impact of OCS on rate of inbreeding and long-term genetic gain based on simulated data (Gourdine et al. 2012; Woolliams et al. 2015) and real data (Hayes et al. 2013; Sanchez-Molano et al. 2016). Gourdine et al. (2012) claimed that the genetic gain with optimal contribution selection could be similar to truncation selection, but the inbreeding was lower. Sanchez-Molano et al. (2016) showed that genome-based optimal contribution strategies could effectively control inbreeding even when selected traits, adaptive traits and production traits, are negatively correlated using simulated data. Henryon et al. (2019) reported that the optimal contribution selection based on pedigree information for controlling inbreeding could achieve more genetic gain than that based on genome information due to less restriction on the change of QTL allele frequencies. Sonesson and Meuwissen (2000) also studied the different mating schemes for optimal contribution selection with constrained rates of inbreeding. Nowadays, there are many ways to measure changes in genetic variation and its diversity. A reduction of genetic diversity can result from inbreeding since it increases homozygosity (Villanueva et al. 2021). We can calculate the inbreeding coefficient if the pedigree information is known. However, in actual situations, the registration of pedigree is often incomplete and inaccurate, limiting the usage of this method. Villanueva et al. (2021) estimated levels of inbreeding obtained from five genomic relationship matrices. Meuwissen et al. (2020) investigated the measures of inbreeding based on homozygosity, genetic drift and identical-by-descent (IBD) for the management of genetic diversity. With the use of molecular markers, more and more genetic diversity indicators are used to assess the degree of diversity of a particular population (Chen et al. 2018; Ebrahimi et al. 2011; Kaljund and Jaaska 2010; Zhao et al. 2019).
The objective of this study is to compare the long-term effects on genetic diversity and genetic gain of using conventional conservation and OCS methods. We achieved this by using a stochastic simulation study approach, where 11 different conservation methods for a small pig population were compared. The results of this simulation study are expected to provide guidance to breeders and government departments on formulating better conservation programs.
Materials and methods
Experimental design
We used stochastic simulation to estimate the long-term genetic gain and genetic diversity using different conservation methods. We simulated random selection and truncation selection as reference methods. The conservation methods included conventional conservation methods and OCS methods. The sires selected were the males with the highest estimated breeding value (EBV) within each sire (half-sib) family for conventional conservation methods. The dams were selected using one of the six methods as below (3–8). We used three OCS methods which differ in the relationship matrix (9–11).
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1.
Random selection
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2.
Truncation selection
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3.
Selecting females with the highest EBV within each full-sib family (Sirehalf -Damfull scenario)
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4.
Selecting females with the highest EBV within each half-sib family (Sirehalf-Damhalf scenario)
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5.
Selecting females with the highest EBV without considering the families (Sirehalf-Damtrunc scenario)
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6.
Randomly sampling females from each full-sib family (Sirehalf-DamfullRandom scenario)
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7.
Randomly sampling females from each half-sib family (Sirehalf-DamhalfRandom scenario)
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8.
Randomly sampling females without considering the families (Sirehalf-DamRandom scenario)
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9.
Optimal contribution selection based on a conventional genomic relationship matrix (GOCS-0 cM, see details in OCS method)
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10.
Optimal contribution selection based on an alternative genomic relationship matrix (GOCS-1cM, see details in OCS method)
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11.
Optimal contribution selection based on pedigree relationship matrix (POCS)
In OCS methods, either pedigree (POCS) or two genomic (GOCS) relationship matrices were used to constrain the rate of inbreeding. In total, there were 11 selection methods studied. Furthermore, we also showed two different penalties of each OCS methods. Therefore, there were 14 scenarios in this study. Each selection method was run for 20 discrete generations, starting from a base population and the animals were selected based on a single trait controlled by 360 quantitative trait loci (QTL). The heritability of the trait was set to 0.2. Furthermore, 36,000 markers were simulated to carry out GOCS. For the methods other than OCS, 12 sires were selected, and each sire was mated to 10 dams in each generation. For OCS methods, the males were allocated 0, 1, 2 … or 120 matings by the program, and females were allocated a single mating in each generation. Each dam produced five offspring with an equal sex ratio. The animals were phenotyped before selection.
OCS methods
POCS allocated matings of selection candidates in generations t = 1…20 according to EBV and pedigree relationships between all the involved animals. It was done by maximizing, Ut, with respect to c (Meuwissen 1997):
where c is an n dimensional vector of genetic contributions, where n is the number of selected candidates, \(\widehat \alpha\) is n vector of EBV, and A is a n × n matrix for selected candidates which is a submatrix from the full additive genetic relationship matrix for all animals in the pedigree. In this study, pedigree of the selected candidates was traced back to the base population (Henderson 1976). Elements of c were constrained to \(0 \le c_i \le 0.5\,\left( {i = 1 \ldots n} \right)\) and the sum of contributions were 0.5 for each sex. The component \({{{\boldsymbol{c}}}}^\prime \widehat \alpha\) is the expected breeding value, and the component \({{{\boldsymbol{c}}}}^\prime {{{\boldsymbol{Ac}}}}\) is the expected average relationship of the proposed offspring. The penalty, ω, is applied to the expected average relationship of the next generation, which was constant across generations. GOCS was performed by replacing A with a n × n genomic relationship matrix (G) which was calculated with genotypes for all markers of all the selected candidates using the method described by Yang et al. (2010). A range of ω (1, 5, 10 … 100) was applied to examine the pattern of genetic gain with different inbreeding rates.
We used also an additional method to build a G matrix for GOCS, where the markers with a distance from a random QTL less than 1 cM were excluded from the G (we call the corresponding GOCS the GOCS-1cM). To differentiate this GOCS method from the conventional GOCS method, we called the classical GOCS method the GOCS-0cM. We used EVA (Berg et al. 2006) to perform POCS and GOCS.
Simulations
Simulations of each conservation method were carried out in three stages: (1) a single founder population was generated as the basis for the subsequent stages, (2) a unique base population was sampled from the last generation of founder population, and (3) a selected population was generated based on the base population. Stage 2 and 3 were run for 100 times to produce 100 replicates. To simplify the simulation, instead of direct calculation of EBV, the EBV was approximated by the breeding values of a genetically correlated pseudo-trait (Dekkers 2007). The genetic correlation was set to 0.6, mimicking a genomic selection with an accuracy of 0.6 (Thomasen et al. 2016).
Founder population and genetic architecture: Generations −2000 to −1
The linkage disequilibrium of QTLs and the markers was generated by simulating a founder population (historical population) with QMSim software (version 1.10) (Sargolzaei and Schenkel 2009) using a Fisher-Wright inheritance model. The population had an effective population size of 200 animals (100 males and 100 females) and 2000 discrete generations. The simulated genome consisted of eighteen 1 Morgan long chromosomes, on which 10,000 loci were equidistantly distributed, resulting in 180,000 loci in total across the genome. The recurrent mutation was allowed at a rate of 2.5 × 10−5 and recombination per chromosome was sampled from a Poisson distribution with a mean of 1.
At the last generation of the founder population (generation −1), among all segregating loci, every second locus with a minor allele frequency (MAF) > 0.05 were used as potential markers. In total, we selected 36,000 markers from these potential marker loci. In total, 360 QTLs were selected from the remaining segregating loci with MAF > 0.01. The QTL allelic effects were assumed to follow a gamma distribution with a shape parameter of 1.48, which was derived from distributing QTL effects in pig breeds (Hayes and Goddard 2001).
Base population: Generation 0
In generation 0, 200 animals were generated by random mating of 100 males and 100 females in generation −1. From these 200 animals, 12 males and 120 females were randomly selected as base animals to produce 600 offspring with an equal sex ratio.
Selected population: Generations 1 to 20
In each of generations 1–20, 120 matings were allocated to sires and dams, and each dam was allocated a single mating to produce five offspring with an equal sex ratio. The offspring in each generation inherited alleles of markers and QTLs from their parents, following Mendel’s laws of heredity allowing for recombinations following a Poisson distribution with a mean of 1. The phenotype of the trait for the ith animal, yi, was calculated as yi = αi + ei, where αi is the animal’s true additive genetic value and ei is the residual environmental value. The true additive genetic value was calculated as the sum of all QTL effects. Those QTL effects were scaled in generation 0 to achieve an initial additive genetic variance equal to the heritability of 0.2. The additive QTL variance explained all additive genetic variance (\(\sigma _\alpha ^2 = \sigma _{qtl}^2\)). Thus the true breeding value (TBV) for an individual was equal to the sum of QTL effects of the individual. The environmental values were sampled from the distribution \(N\left( {0,\,\sigma _e^2 = 1 - h^2} \right)\). The environmental variance \(\sigma _e^2\)was constant through the generations of the simulation, such that genetic variance and heritability decreased throughout the generations of selection due to random drift, fixation, and Bulmer-effect (Bulmer 1971).
Tracing identity-by-descent
To compute the rate of true inbreeding, 2000 identical-by-descent (IBD) loci were equidistantly placed on each chromosome of animals in the base populations. Unique alleles were assigned to these IBD loci in the base population to trace each base animal’s contribution to their descendants (Liu et al. 2017). A descendant was IBD at an IBD locus when it inherited two copies of a unique allele. These IBD loci were not used for prediction or selection.
Statistical analyses
Rate of genetic gain and inbreeding
The rates of genetic gain and the rates of true inbreeding are presented as means (±SD) of the 100 replicates. The rate of genetic gain in each replicate was calculated as a linear regression of Gt on t, where Gt is the average TBV of animals born in generation, t = 1…20 in each replicate. The rate of inbreeding was calculated as 1−exp(β), where β is a linear regression coefficient of ln(1−Ft) on t, Ft was the average true inbreeding coefficient of all the individuals born in generation t (t = 1…20), and the inbreeding coefficient of each individual was calculated as the proportion of IBD loci being IBD to total IBD loci in the genome (Liu et al. 2017).
Genetic diversity metrics
We calculated the following genetic diversity metrics.
Expected heterozygosity (He) is the probability that an individual will be heterozygous at a given locus in one population. It is calculated by Nei’s (1973) method as follows:
Observed heterozygosity (Ho) refers to the ratio of the observed heterozygous individuals in the population to the total number of individuals. The calculation formula is as follows:
where n is the number of individuals in the population, N is the total number of loci, Hk is the number of individuals with locus K is heterozygous and Pki is the probability of allele i at locus K.
The number of the polymorphic gene loci (M01 and M05) is defined as the minor allele frequency of a gene locus is larger than or equal to 0.01 or 0.05 (Ayala et al. 1972).
Effective allele number (Ae) in one population is calculated by \(A_e = 1/p_i^2\), where pi is the frequency of the i-th allele of the gene locus (Nei 1973).
Results
The rate of genetic gain and rate of true inbreeding
We presented long-term response frontiers by plotting the rate of genetic gain against the rate of inbreeding with all possible solutions by applying different penalties for POCS and GOCS. As shown in Fig. 1, the rate of true inbreeding for all the conventional methods was around 0.01, except for the truncation selection scenario, consistent with basic conservation theory. Most importantly, inbreeding increment of each generation in the random scenario was also around 0.01, which indicates that the rate of true inbreeding could also be controlled around 0.01 as long as we maintain an appropriate population size (such as12 males and 120 females in this study) and guarantee complete random mating. Among these conventional conservation methods, when the inbreeding increment was 0.01, the scenarios of Sirehalf-Damtrunc, Sirehalf-Damhalf, and Sirehalf-Damfull obtained higher genetic gains. However, the genetic gain was much smaller when dam was randomly selected in three various forms (i.e., Sirehalf-DamfullRandom, Sirehalf-DamhalfRandom and Sirehalf-DamRandom). As expected, no genetic gain was obtained when both sire and dam were randomly selected. Compared with the six conservation methods, truncation selection on both sire and dam increased genetic gain by 7.5% (vs. Sirehalf-Damtrunc) to 67.5% (vs. Sirehalf-DamfullRandam), but it tripled the rate of inbreeding.
All the OCS methods based on the genome and pedigree information realized more genetic gain than the conventional conservation methods when the inbreeding rate was almost around 0.01 (Fig. 1 and Table 1, p = 10). Interestingly, there was no significant difference in both the rate of true inbreeding and genetic gain between these two GOCS methods (GOCS-0cM and GOCS-1cM). However, POCS could achieve more genetic gain than GOCS when the inbreeding rate was the same. POCS with penalty p = 10 obtained the rate of genetic gain as high as the truncation scenario, but the rate of inbreeding was only one third. The rate of inbreeding of two GOCS methods and POCS were similar to that of the Sirehalf-Damtrunc scenario when the penalty p = 7, which used the same number of sires (Fig. 6), but they would be similar to that of the four methods (Sirehalf-Damfull, Sirehalf-DamfullRandom, Sirehalf-Damhalf and Sirehalf-DamhalfRandom) when the penalty p = 10.
Changes in genetic diversity of different methods
In terms of He and Ho, as shown in Figs. 2a and b, 3a and b, GOCS methods were better than the conventional conservation method when the penalty p was increased to 10. As the number of generations increases, the declined slope of the He and Ho was smaller in GOCS methods compared to the conventional conservation methods, which indicated that GOCS had better effect than that of the conventional conservation methods. However, POCS was not superior to the conventional conservation methods and was only better than the truncation scenario, in terms of He and Ho. Furthermore, there was no significant difference in He and Ho among the conventional conservation methods.
Figures 2c, 3c and Table 2 showed that there were more effective alleles in two GOCS scenarios when the weight p was 10. Regardless of p = 7 or p = 10, the POCS led to low Ae (see Fig. 3c, Table 2 and Additional file 2), only higher than the truncation scenario. For M01 and M05 (Figs. 2d, 3d and Additional file 1), several conventional conservation methods, such as Sirehalf-Damfull, Sirehalf-DamfullRandom, Sirehalf-Damhalf, and Sirehalf-DamhalfRandom, were better than those of OCS methods.
Changes of additive genetic variances across generations are presented in Fig. 4. From Fig. 4, the additive genetic variance in optimal contribution selection methods had the fastest decline, compared with other scenarios except for the method of truncation selection on both sire and dam. The variance in the POCS method was lower than that in GOCS methods. The four conventional scenarios with different types of random selection had the highest additive genetic variance, and the order was Random, Sirehalf-DamfullRandom, Sirehalf-DamhalfRandom, and Sirehalf-DamRandom. The trends of additive variance and inbreeding were generally inversely consistent.
Number of ancestors and sires used
As for the number of ancestors for different methods (Fig. 5), the pattern of the number of ancestors in the Sirehalf-Damfull and Sirehalf-DamfullRandom methods were different from the other methods. These two methods remained the same number of ancestors in the first generations. The trends began to decline after the fifth generation, indicating that some ancestors failed to make contributions as selection proceeds due to selection and genetic drift. For other methods, the number of ancestors declined rapidly in the previous generations, and then gradually flattened. Therefore, keeping the same number of offspring from each sire and dam family will have the best effect in the first few generations. In addition, Sirehalf-Damfull and Sirehalf-DamfullRandom methods also retained the largest number of ancestors in the last few generations. The second largest number of ancestors was observed in the OCS scenarios, including GOCS-0cM, GOCS-1cM, and POCS.
The number of sires was around 12, which is the same as in conservational methods, when the weight p was 7 in two GOCS scenarios (Fig. 6). However, when the same number of sires were included, the weight p could be about 6 in the POCS scenario. When the weight p was 7 in the POCS scenario, the number of sires was around 13. This may indicate POCS method needs to use little more sires to maintain the same level of genetic diversity or the rate of inbreeding compared to the GOCS method, although POCS could achieve more genetic gain. All OCS methods used more sires when the rate of true inbreeding was the same as in conventional methods. The number of sires used was about 18 in POCS and about 16 in two GOCS methods.
Discussion
In this study, we studied conventional conservation and optimal contribution scenarios to conserve indigenous pig breeds with small population sizes using simulation studies. We explored the genetic diversity changes and genetic gain of these conservation scenarios during 20 generations. These findings are helpful in guiding the current conservation programs. To utilize indigenous pig breeds for pig production, genetic improvements for some important economic traits in conserved pig populations is necessary. In the current study the genetic gain obtained by the optimal contribution selection methods show a trend of increasing with increasing weight p when p was small, and then decreases as the weight p increases. This may be because the increase in selection intensity with small p accelerates the reduction of genetic variation within the population, thereby reducing the further improvement of genetic gain. This implies that selection without a restraint on inbreeding will lead to the selection limit (Zeng and Hill 1986). Long-term high-intensity selection will reduce the population’s genetic variation, and the reduction of genetic variation will counteract the increase in genetic gain (Vanavermaete et al. 2020). In addition, from the changes in genetic diversity of different scenarios, we could see that the penalty on genetic relationship should at least 10 if we want to apply GOCS methods for maintaining a higher heterozygosity than the conventional conservation scenarios.
As expected, the truncation method caused largest rate of inbreeding (Fig. 1, Table 1), and the trend became very significant as the number of generations increased. It indicates that we should not use this method to conserve indigenous pig breeds when the population size is small among conservation farms, which is different from selecting and breeding in the breeds for commercial production. Using conventional conservation methods, the trends of rate of inbreeding were almost the same in the scenarios with different methods of selection on dam, except for truncation selection. This indicates that selection of dam within full-sib or half-sib family or random selection of dam from the whole population could all be used in actual conservation operation. The most important thing is that the boars should come from each sire family. Relaxing the restriction on dams can benefit to the farm management by reducing farmers’ workload. Therefore, this result could guide the actual conservation operation.
Different indicators have been used to measure genetic diversity. Each indicator has its advantages and disadvantages (Allendorf 1986). Ayala et al. (Ayala and Kiger 1984) summarized the study on genetic diversity of main domestic animals and indicated that the ratio of polymorphic loci and the average expected heterozygosity were the primary parameters to measure genetic diversity. Qian et al. (2001) reported that the degree of expected heterozygosity was more effective than the ratio of polymorphic loci in accuracy of measuring genetic diversity. The variation of the number of polymorphic loci is relatively small, and the sensitivity to genetic diversity is relatively low (Qian et al. 2001). In addition, the number of effective alleles could more effectively measure the change of genetic diversity in one population (Nei 1973). Therefore, in this study, we used multiple indicators to measure the impact of different conservation methods on genetic diversity changes after a number of generations to make our results more comprehensive and objective to a certain extent. These indicators could complement each other. For each indicator of genetic diversity, the results of GOCS-0cM and GOCS-1cM were similar. As the results show in Fig. 4, the additive genetic variance in OCS methods had the fastest decline, which indicates the OCS methods results in larger increases in the frequencies of favorable alleles at QTL, compared with the other methods. Moreover, POCS is larger than GOCS, which is also consistent with the previous study (Henryon et al. 2019). The results of the number of polymorphic loci such as M01 and M05 (Fig. 3d, Additional file 1) also illustrate this point. When the penalty is 7, the optimal contribution selection methods (POCS and GOCS) were not better than several conventional conservation methods such as Sirehalf-Damfull, Sirehalf-DamfullRandom, Sirehalf-Damhalf, and Sirehalf-DamhalfRandom. This may be also due to the selection of QTLs that affect the traits, and the directional selection decreases polymorphic loci ratio.
From OCS methods, we can see that the genetic gain obtained by the optimal contribution selection method based on the pedigree relationship (POCS) is higher than that obtained based on genomic relationship (GOCS) when the rate of inbreeding in each generation is controlled at about 0.01. OCS automatically determines the number of male animals required to control inbreeding (Henryon et al. 2019). Through comparison (Fig. 6), it is found that POCS method requires more sires than GOCS when the rate of inbreeding is controlled in the same level. In actual pig production, POCS method is often easier to put into practice. POCS method is based on pedigree information, and it only requires that the pedigree of each animal is registered in the conservation farms. Unlike POCS, GOCS method is based on genomic information, which requires individuals’ genotype data. Thus, the cost is relatively high for conservation farms. It is impossible to genotype all individuals to obtain genotype data for general conservation farms. Therefore, if keeping little more males is acceptable by conservation farms, POCS method is a better way if we want to obtain more genetic gain. However, if we want to achieve the balance of conservation and selection, GOCS is a better choice, which allows to both control inbreeding and improve economic traits, compared to the other conservation methods.
Many factors could influence the objectives of a conservation or breeding program, such as economical value, historic bottlenecks, and the maintenance of genetic diversity level (Wellmann 2019). Nowadays, we pay more attention to increase economic merit for most livestock breeds. Thus the most critical breeding objective is to maximize genetic gain (Vanavermaete et al. 2020). However, for example, historic bottlenecks are commonly suffered in companion animals (Marsden et al. 2016) because of an overuse of elite males. In such situation, the priority is to minimize inbreeding in these animal populations. These goals conflict with each other to a certain extent. In order to maximize genetic gain, people would prefer to choose the animals with the highest breeding values for economic traits (Vanavermaete et al. 2020), which will increase rate of inbreeding, and may lead to serious inbreeding depression and new bottlenecks. On the other hand, only focusing on maintaining genetic diversity is not conducive to the economic benefits of the conservation farms.
Additive genetic effects can be transferred from parents to progeny and it plays the most important role in animal genetic breeding at present, so we mainly investigated the effects of different methods on genetic diversity and genetic gain using the additive genetic model in this study. Adding dominance and epistatic effects might change the values of population mean and heterosis in all scenarios (Shapira and David 2016), but the ranking of the selection methods regarding realized genetic gain and genetic diversity should not be changed. However, it is worthy of a further study to investigate the effect of various selection methods on non-additive genetic value of population.
It is important to protect and conserve the indigenous pig breeds, especially when their population size has dropped sharply (Oldenbroek 2017). However, it is not sensible and conducive if we only focus on protecting but not improving traits we are interested in. The current study shows that the optimal contribution selection method based on genomic information can maintain a high genetic diversity across the whole genome while improving the traits of interest.
Conclusion
In conclusion, our study showed conventional conservation scenarios resulted in the rate of inbreeding for each generation was at around 0.01. Different methods to select sows have small impact on inbreeding when we use conventional conservation methods. Compared with conventional methods, optimal contribution selection based on the pedigree relationship (POCS) method could achieve the most genetic gain. However, optimal contribution selection based on the genomic relationship methods (both GOCS-0cM and GOCS-1cM) can not only achieve higher genetic gain, but also maintain a relatively high level of genetic diversity, and the results of these two GOCS methods are similar. In particular, the advantages of GOCS in maintaining genetic diversity becomes more obvious as the number of generations increases. Therefore, GOCS is a better choice if we want to combine conservation and genetic progress in actual production in the conservation farms, although it needs a cost in genotyping. We can also choose whether to obtain higher genetic gain or maintain a higher level of genetic diversity according to our needs, and then appropriately adjust the conservation strategy according to our different concerns and goals.
Data availability
The authors are not free to distribute the source code of the simulation program. Please contact the corresponding author if you need it.
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Acknowledgements
The authors are grateful to the National Natural Science Foundation of China. This work was supported by the National Natural Science Foundation of China (grant nos. 31772552, 31941007, 31872321). The first author acknowledges the scholarship provided by the China Scholarship Council (CSC). We also thank the three anonymous reviewers for their constructive suggestions and comments on our manuscript.
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YP, GS, QZ, and HL conceived the study. YP and GS supervised the study, while QZ and HL ran the simulation, analyzed the data, and wrote the main manuscript. QZ, GS, and HL interpreted the results. All authors gave necessary suggestions, revised and approved the final manuscript.
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Zhao, Q., Liu, H., Qadri, Q.R. et al. Long-term impact of conventional and optimal contribution conservation methods on genetic diversity and genetic gain in local pig breeds. Heredity 127, 546–553 (2021). https://doi.org/10.1038/s41437-021-00484-z
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DOI: https://doi.org/10.1038/s41437-021-00484-z
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