The reproducibility and reliability of ELISA assays used to detect autoantibodies that recognize cardiolipin or β2-glycoprotein I remain unacceptable, which can complicate the diagnosis of antiphospholipid syndrome. Now, much needed international consensus guidelines have been proposed for the development and use of these diagnostic tests, which should improve their performance.
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References
Lakos, G. et al. International consensus guidelines on anticardiolipin and anti-β2 glycoprotein I testing: a report from the APL Task Force at the 13th International Congress on Antiphospholipid Antibodies. Arthritis Rheum. http://dx.doi.org/10.1002/art.33349.
Miyakis, S. et al. International consensus statement on an update of the classification criteria for definite antiphospholipid syndrome (APS). J. Thromb. Haemost. 4, 295–306 (2006).
Pengo, V. et al. Update of the guidelines for lupus anticoagulant detection. J. Thromb. Haemost. 7, 1737–1740 (2009).
Jennings, I. et al. Lupus anticoagulant testing: improvements in performance in a UK NEQAS proficiency testing exercise after dissemination of national guidelines on laboratory methods. Br. J. Haematol. 119, 364–369 (2002).
Reber, G., Boehlen, F. & de Moerloose, P. Technical aspects in laboratory testing for antiphospholipid antibodies: is standardization an impossible dream? Semin. Thromb. Hemost. 34, 340–346 (2008).
Macdonald P. M. & Seelig, J. Calcium binding to mixed cardiolipin–phosphatidylcholine bilayers as studied by deuterium nuclear magnetic resonance. Biochemistry 26, 6292–6298 (1987).
de Groot, P. G., Horbach, D. A., Simmelink, M. J., van Oort, E. & Derksen, R. H. Anti-prothrombin antibodies and their relation with thrombosis and lupus anticoagulant. Lupus 7 (Suppl. 2), S32–S36 (1998).
de Groot, P. G. & Meijers, J. C. β2-glycoprotein I: evolution, structure and function. J. Thromb. Haemost. 9, 1275–1284 (2011).
Ioannou, Y. et al. Novel assays of thrombogenic pathogenicity in the antiphospholipid syndrome based on the detection of molecular oxidative modification of the major autoantigen β2-glycoprotein I. Arthritis Rheum. 63, 2774–2782 (2011).
Pengo, V., Biasiolo, A., Bison, E., Chantarangkul, V. & Tripodi, A. Antiphospholipid antibody ELISAs: survey on the performance of clinical laboratories assessed by using lyophilized affinity-purified IgG with anticardiolipin and anti-β2-glycoprotein I activity. Thromb. Res. 120, 127–133 (2007).
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de Groot, P., Urbanus, R. Guidelines for antiphospholipid antibody detection. Nat Rev Rheumatol 8, 125–126 (2012). https://doi.org/10.1038/nrrheum.2011.188
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DOI: https://doi.org/10.1038/nrrheum.2011.188