Abstract
Replying to H. Vais, J. K. Foskett & D.-O. D. Mak Nature 478, doi:10.1038/nature10493 (2011)
We observe that under conditions designed to mimic those of an unstimulated cell, InsP3 causes InsP3R to assemble rapidly and reversibly into small clusters of about 4–5 channels, and that gating of lone and clustered InsP3R by InsP3 and Ca2+ is different. We speculate that dynamic assembly of InsP3R clusters by InsP3 may contribute to the genesis of elementary Ca2+ release events by both assembling clusters and re-tuning the behaviour of InsP3R within them1,2,3. Vais et al.4 report that they have been unable to replicate some of our findings.
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References
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T.R. performed the experiments and, with C.W.T., analysed the data. C.W.T. and T.R. wrote the paper with input from A.S. and M.F. The project was directed by C.W.T.
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Rahman, T., Skupin, A., Falcke, M. et al. Rahman et al. reply. Nature 478, E2–E3 (2011). https://doi.org/10.1038/nature10494
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DOI: https://doi.org/10.1038/nature10494
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