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Gore et al. reply

Nature volume 450, pages E2–E4 (2007)Cite this article

Abstract

Replying to: J. T. Bennett et al. Nature 450, doi:10.1038/nature06314 (2007)

We presented several lines of evidence indicating that dorso–ventral asymmetry is apparent in zebrafish embryos by cleavage stages1, one of which showed that injection of three different morpholino oligonucleotides targeting three different squint (sqt) sequences cause severe disruption in dorsal structures. We concluded that the dorsal axis is evident by the 4-cell stage, and suggested that maternal Sqt and associated factors may function in zebrafish dorsal-axis formation1. Bennett et al. challenge our results obtained with morpholino oligonucleotides because they do not find a comparable defect in maternal and zygotic sqt mutants2.

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Figure 1: Unspliced sqt RNA is present in zebrafish ovaries, eggs and embryos.

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Author notes

  1. Aniket V. Gore & Albert Cheong

    Present address: †Present addresses: Unit on Vertebrate Organogenesis, Laboratory of Molecular Genetics, The National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA (A.V.G.); Cell Culture Process Development, Lonza Biologics, 228 Bath Road, Slough, Berkshire, SL1 4DX, UK (A.C.),

Authors and Affiliations

  1. Vertebrate Development Group, Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, 117604 Singapore

    Aniket V. Gore, Albert Cheong, Patrick C. Gilligan & Karuna Sampath

  2. † School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, 637551 Singapore ,

    Karuna Sampath

  3. Department of Biological Sciences, National University of Singapore, 117543 Singapore

    Karuna Sampath

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  1. Aniket V. Gore
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Gore, A., Cheong, A., Gilligan, P. et al. Gore et al. reply. Nature 450, E2–E4 (2007). https://doi.org/10.1038/nature06315

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