This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 12 digital issues and online access to articles
$119.00 per year
only $9.92 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Data availability
The cryo-EM density maps have been deposited in the Electron Microscopy Data Bank (EMDB) and Protein Data Bank (PDB) under accession numbers EMD-36486 and 8JPP for SA–SUCR1–miniGs/q complex; and EMD-36484 and 8JPN for ESA–SUCR1–Gi complex.
References
Mills, E. & O’Neill, L. A. Trends Cell Biol. 24, 313–320 (2014).
Pardella, E., Ippolito, L., Giannoni, E. & Chiarugi, P. FEBS Lett. 596, 2364–2381 (2022).
He, W. H. et al. Nature 429, 188–193 (2004).
Murphy, M. P. & Chouchani, E. T. Nat. Chem. Biol. 18, 461–469 (2022).
Trauelsen, M. et al. Cell Rep. 35, 109246 (2021).
Keiran, N. et al. Nat. Immunol. 20, 581–592 (2019).
Macaulay, I. C. et al. Blood 109, 3260–3269 (2007).
Schneider, C., O’Leary, C. E. & Locksley, R. M. Nat. Rev. Immunol. 19, 584–593 (2019).
Wu, J. Y. et al. Mol. Cell 77, 213–227 (2020).
Regard, J. B., Sato, I. T. & Coughlin, S. R. Cell 135, 561–571 (2008).
Haffke, M. et al. Nature 574, 581–585 (2019).
Duan, J. et al. Nat. Commun. 11, 4121 (2020).
Yang, Y. et al. Nat. Commun. 14, 1692 (2023).
Shenol, A. et al. Mol. Cell 84, 955–966 (2024).
Zhao, C. et al. Mol. Cell 83, 3171–3187 (2023).
Acknowledgements
We acknowledge support from the National Natural Science Foundation of China (22121003, 22337006, 32027901 and 21927814), the National Key R&D Program of China (2019YFA0904201, 2023YFA0916300, 2021YFA0910202 and 2022YFA1304701), the Strategic Priority Research Program of Chinese Academy of Sciences (XDB37040203) and the CAS Project for Young Scientists in Basic Research (YSBR-015 and YSBR-072-6). We thank J. Liu, N. Chen, S. Hu, L. Jiang, and Z. Fan from Insect Cell Expression Core and P. Si and X. Liu from Mammalian Cell Expression Core for Biomass production. The cryo-EM data were collected at the Bio-Electron Microscopy Facility of ShanghaiTech University with the assistance from L. Wang, D. Liu, Q. Sun, and Z. Zhang. This research also got support from Protein Purification Core, Cloning and Assays Cores of iHuman Institute.
Author information
Authors and Affiliations
Contributions
J.W. organized the project. F.L., W.T., and X.S. guided all the structural analysis. F.L. designed all the mutants for functional analysis. T.W., J.C., Y.L., H.L., and Q.G. developed the SUCR1 constructs and optimized protein expression. T.W. and J.C. performed protein expression, prepared samples for cryo-EM grids, and collected the cryo-EM data. W.T. performed cryo-EM map calculation, model building, structure refinement, and MD simulations. Z.L. and X.Z. performed the functional assay. J.W. and F.L. wrote the manuscript. All the authors have read and commented on the manuscript.
Corresponding authors
Ethics declarations
Competing interests
The authors declare no competing interests.
Supplementary information
Rights and permissions
About this article
Cite this article
Wang, T., Tang, W., Zhu, X. et al. Molecular activation and G protein coupling selectivity of human succinate receptor SUCR1. Cell Res (2024). https://doi.org/10.1038/s41422-024-00968-7
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/s41422-024-00968-7