Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain
the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in
Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles
and JavaScript.
This protocol uses CuAAC click chemistry along with two different methods for expansion of the genetic code to assemble protein-protein conjugates for studying the post-translational modification of proteins by ubiquitin (ubiquitylation).
In this protocol, limb muscles are physically and enzymatically dissociated to maximally release resident mononucleated cells. Pure populations of either quiescent or activated muscle stem cells are then isolated by flow cytometry.
In this protocol, neuronal miRNAs and transcription factors are used to directly convert human fibroblasts to striatal medium spiny neurons, a neuronal subtype important in motor control and the main cell type affected in Huntington's disease.
Unbiased proteome-level discovery of intracellular drug targets can be achieved by plotting melting curves of proteins from untreated and drug-treated cells. Multiplexed quantitative mass spectrometry using TMT10 reagents makes this possible.
This protocol describes how to prepare multifunctional DNA nanoflowers. Rolling-circle amplification of a designed template containing drug binding, cell targeting, and fluorescent dye binding sites creates structures of controllable size for drug delivery.
The Gonzalez laboratory provides its allograft protocol for studying tumorigenesis in Drosophila. Transplanting tissue from donor larvae to adult hosts allows its tumorigenic potential to be determined.
The study of meiosis in plants is considered to be gender-biased owing to the easy accessibility of male meiocytes. This protocol describes how to prepare and image female Arabidopsis meiocytes to investigate protein localization during meiosis.
This protocol describes how to set up an assay investigating vessel outgrowth from mouse fetal metatarsals. This assay is an ex vivo assay to investigate sprouting angiogenesis.
This protocol describes how to grow untransformed human colonic organoids and deliver genes of interest into the organoids via the piggyBac transposon or gene editing using the CRISPR-Cas9 system.
The Stelzer lab describes how to live-image Tribolium embryos using light-sheet-based fluorescence microscopy (LSFM). Imaging can proceed for up to 120 h, allowing the entire embryonic development of this important model insect to be recorded.
EmRiboSeq determines the precise location of embedded ribonucleotides in the S. cerevisiae genome, tracking DNA polymerase activity in vivo. An adaptation of this protocol, EndoSeq, also allows the genome-wide mapping of other noncanonical bases.
Single-cell analysis has shown that a lot of information can be lost by analyzing homogenates of tissues. This protocol describes how to remove the contents of a single plant cell and directly analyze the metabolites by mass spectrometry.
18F is commonly used for preparation of probes for position-emission tomography. This protocol is for the modular synthesis of radiolabeling precursors via a copper-catalyzed 'click' reaction followed by a one-step 18F labeling.
This protocol describes flow cytometry panels that can be used to analyze and isolate dendritic cell (DC) progenitors including granulocyte, monocyte and DC progenitor cells; monocyte and DC progenitor cells; and common DC progenitor and DC precursor cells.
Sumoylation is a post-translational modification involved in regulating many cellular processes. This protocol is for detecting sumoylation sites in cultured cells using a diGly-Lys (K-ɛ-GG)–specific antibody and mass spectrometry–based proteomics.
This protocol enables cell-free synthesis of membrane proteins using the PURE system, subsequent quantification of products and analyses of membrane localization efficiency, product orientation, and complex formation in the membrane.
Silk-collagen protein scaffolds are seeded with rat primary cortical neurons, which grow neuronal projections that form neuronal networks and functional connectivity.
This protocol describes how to generate large quantities of cardiomyocytes from human pluripotent stem cells using a bioreactor. Cells are differentiated by application of Wnt pathway modulators while growing in suspension culture.