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This protocol describes how to record intracellular neuronal activity in awake nonhuman primates in response to external stimuli. With the help of a coaxial guide tube, multiple neurons can be recorded over longer times from a single individual.
Here, the authors describe step-by-step procedures for integrating single-cell sequencing datasets from different experiments or modalities to identify common and distinct cell types using the R-based software tool LIGER.
This protocol describes the light-induced functionalization of proteins with compounds bearing the photochemically active aryl azide group. The procedure is exemplified by the light-induced radiosynthesis of zirconium-89 radiolabeled mAbs.
The authors describe how to easily prepare a large number of dipsticks from cellulose-based filter paper and use them to rapidly purify nucleic acids from a variety of sources.
The authors describe an approach for optimizing transcranial magnetic stimulation (TMS) targeting by combining functional magnetic resonance imaging and iterative electric-field stimulation.
This protocol describes a comprehensive computational pipeline for reference-free deconvolution of bulk DNA methylation data, including data preprocessing, confounder adjustment, feature selection, and visualization and interpretation of the results.
The production and titration of the SARS-CoV-2 S pseudotyped virus using a VSV-based pseudovirus production system in this protocol enable its use under biosafety level 2 conditions as well as in a neutralization assay to assess the level of neutralizing antibodies or molecular inhibitors in a sample.
Solid acid catalysts are used in a wide variety of industrial catalytic processes for production of chemicals and petrochemicals. This protocol uses 31P NMR of phosphorous probes to characterize acidity in detail for both Brønsted and Lewis acid sites.
This protocol describes the synthesis of two fluorescent probes for (sub-)cellular detection of endogenous formaldehyde, including procedures for probe characterization and example applications in living cells and mouse tissue slices.
This homology-directed insertion-based CRISPR gene-editing protocol enables knockout of all alleles of a target gene in the polyploid Drosophila S2R+ cell line, using either two sequential rounds of homology-directed insertion or a single round with a donor vector containing four different sgRNAs.
This protocol summarizes the various approaches available to derive organoids from cancer patients and use these for screening of possible treatments. An optimized protocol for using head and neck cancer organoids is also described.
The authors describe a streamlined epigenomic profiling protocol based on cut-and-paste tagmentation by the Tn5 transposase targeted to a chromatin protein of interest.
This protocol describes a combined approach for whole-mount fluorescence in situ hybridization (FISH) and immunofluorescence staining in zebrafish embryos and larvae.
This protocol describes how to isolate trophoblast from first-trimester human placentas and grow it long term in a three-dimensional organoid culture system.
This protocol describes a toolbox for comprehensive characterization of inflammasome activation and cell death in response to both in vivo (in mice) and in vitro (using bone marrow–derived macrophages) models of infection, sterile insults, and cancer.
The authors describe a standardized three-chamber social preference protocol that is sensitive and reliable at detecting social preference deficits in several mouse models of autism spectrum disorder.
Precise knowledge of the structure of metals supported and stabilized by zeolite frameworks informs improved design and synthesis of the catalysts. This tutorial describes scanning transmission electron microscopy approaches for their structural analysis.
The authors describe a platform to screen arrays of designer epigenome modifiers (DEMs) targeting up to three genes of interest using a multicolor reporter cell line to determine combinations producing the most efficient epigenome editing.
This protocol provides a detailed guide to metaproteomics data analysis and visualization. The modular and customizable workflow is based on two open-source tools (MetaProteomeAnalyzer and Prophane) and is illustrated with example datasets.
Mesoporous metal films are used in many electrochemical applications. This protocol describes how to make mesoporous Au, Pt and Pd films using micelle templates and provides examples of how to use them (glucose detection and catalysis for alcohol oxidation).