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This protocol describes proximity labeling approaches using TurboID and split-TurboID, which can be used for mapping protein–protein interactions and organelle proteomes in live mammalian cells with nanometer spatial resolution.
This protocol describes experimental and computational procedures for genome-wide detection of endogenous and induced DNA double-strand breaks (DSBs) in any cell type or tissue that can be brought into suspension.
Label-free and reagentless analysis of biomarkers is ideal for point-of-care diagnostics. In this protocol, changes in electrochemical capacitance resulting from biomarker binding to an electrode coated with a biological receptor are detected.
In this Perspective, the authors discuss strategies for creating a versatile set of 30 genetically engineered mouse models (GEMMs) useful for coronavirus disease 2019 research. In addition, they provide the genetic blueprints needed for developing these GEMMs.
This protocol describes how to generate oligodendrocyte precursor cells from human pluripotent stem cells that can subsequently myelinate neurons, both in vitro and in vivo.
This protocol assesses proinflammatory properties of nucleic acid nanoparticles (NANPs) in PBMCs, which are highly predictive of cytokine responses. The authors detail how to prepare NANPs and analyze characteristic biomarkers produced by PBMCs transfected with NANPs.
RepeatExplorer is a software tool for repeat identification and quantification using unassembled sequencing reads. The authors describe four pipelines implemented on the Galaxy platform, highlighting different applications.
This tutorial describes how to design nanoparticle-based LFAs for detecting biomolecules. The authors provide guidance on how to select the appropriate lateral-flow strip components and bioreceptors as well as detection strategies.
This tutorial describes a set of essential performance tests for characterization of neural interface electrodes. The authors provide guidelines for standardized implementation and reporting on electrode performances.
This protocol describes how to record intracellular neuronal activity in awake nonhuman primates in response to external stimuli. With the help of a coaxial guide tube, multiple neurons can be recorded over longer times from a single individual.
Here, the authors describe step-by-step procedures for integrating single-cell sequencing datasets from different experiments or modalities to identify common and distinct cell types using the R-based software tool LIGER.
This protocol describes the light-induced functionalization of proteins with compounds bearing the photochemically active aryl azide group. The procedure is exemplified by the light-induced radiosynthesis of zirconium-89 radiolabeled mAbs.
The authors describe how to easily prepare a large number of dipsticks from cellulose-based filter paper and use them to rapidly purify nucleic acids from a variety of sources.
The authors describe an approach for optimizing transcranial magnetic stimulation (TMS) targeting by combining functional magnetic resonance imaging and iterative electric-field stimulation.
Neural interfaces with implantable electrodes are used to modulate and restore function to the peripheral nervous system. Hybrid modeling described in this protocol is used to optimize each aspect of the implantable electrode design and operation.
This protocol describes a comprehensive computational pipeline for reference-free deconvolution of bulk DNA methylation data, including data preprocessing, confounder adjustment, feature selection, and visualization and interpretation of the results.
The production and titration of the SARS-CoV-2 S pseudotyped virus using a VSV-based pseudovirus production system in this protocol enable its use under biosafety level 2 conditions as well as in a neutralization assay to assess the level of neutralizing antibodies or molecular inhibitors in a sample.