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Long-read sequencing and chromatin capture methods reveal that two nuclear genomes segregate among thousands of co-existing nuclei in arbuscular mycorrhizal fungi.
A citizen-science approach is used to map the vaginal microbiome of 3,345 women and identify associations with health, life-course and lifestyle factors.
Sperschneider et al. generate nuclear-assigned genome assemblies for several isolates of the wheat leaf rust pathogen, Puccinia triticina, and show that repeated nuclei shuffling between clonal lineages has created global populations.
The Plasmodium falciparum transcription factor PfAP2-P is an upstream regulator of parasite blood-stage development and regulates trophozoite development, host cell remodelling, antigenic variation and pathogenicity.
A multi-omics approach reveals that bifidobacteria metabolize the prebiotic lactulose to produce acetate and deconjugate bile acids, which is associated with reduced densities of drug-resistant pathogens and decreased incidences of infection in patients with liver disease.
Mpox virus productively infects human induced pluripotent stem cell-derived skin organoids and is inhibited by tecovirimat, revealing a model system for analysis of mpox virus pathogenesis and drug screening.
Genomic fitness profiling of Acinetobacter baumannii treated with ten clinically relevant biocides using TraDIS reveals a common mechanism of action through dissipation of membrane potential that can promote antibiotic tolerance.
Computational, experimental and structural analyses reveal an abundant, nucleoid-associated histone and an alternative mode for DNA binding in vitro in the bacterium Bdellovibrio bacteriovorus.
Genomic epidemiology of Vibrio cholerae isolates recovered between 2016 and 2019 during the Yemen cholera outbreak reveals acquisition of multidrug resistance and patterns of plasmid transmission between endemic and epidemic lineages.
Culture-independent CRISPR spacer screens enable the discovery of hundreds of viruses and mobile genetic elements infecting all known methanogenic lineages.
Pangolin coronavirus GD has reduced replication and transmission fitness compared with SARS-CoV-2, and therapies approved for SARS-CoV-2 are effective against this virus.