Reviews & Analysis

Algorithms from the Nesvizhskii and Smith groups facilitate faster and more comprehensive glycopeptide assignments from mass spectrometry datasets.

This Review on nanothermometry introduces the many types of nanothermometers and their cellular and in vivo applications, as well as best practices for accurate measurements.

Plankton regularly travel vast distances up and down in the ocean. A water-filled hamster wheel with glass windows now enables detailed microscopic lab observations of individual aquatic microorganisms during their vertical migrations.

This Review focuses on protease-based tools for sensing and controlling protein function in cell biology.

A review on recent method development for discovering T cell antigens.

Producing reliable atomic- or close-to-atomic-resolution structures of RNA-only molecules has been a formidable task. Ribosolve can solve sub-nanometer-resolution cryo-EM structures of unbound RNA molecules with unprecedented accuracy and speed.

This Perspective highlights recently developed computational models for studying chromosome organization, with a focus on how mechanistic modeling helps biologists to interpret the biological function behind the genome structures.

This Perspective reviews tools developed over the past five years in the macromolecular modeling, docking and design software Rosetta.

This Review discusses temporal focusing microscopy and its applications in neuroscience for imaging and optogenetic activation.

A resource of detailed DNA delivery and expression protocols for marine protists will enable new studies to understand the fundamental and ancestral features of eukaryotic cells.

This Review highlights recent discoveries and applications of anti-CRISPR (Acr) proteins that enable the regulation of CRISPR-Cas technology.

This Perspective discusses the challenges, progress and future of proteome profiling at specific chromatin loci for elucidating genome biology.

This Perspective highlights how high-resolution imaging has informed our view and helped overturn the textbook understanding of 4D genome organization.

New methods from the Heo and Johnsson labs enable light- and drug-inducible control of intrabody binding in cells.

This Perspective describes the development and capabilities of SciPy 1.0, an open source scientific computing library for the Python programming language.