Reviews & Analysis

A brief overview of how to solve a macromolecular structure using single-particle cryo-electron microscopy (cryo-EM).

This review describes methods for increasing the activity and accuracy of the CRISPR-Cas9 system and discusses approaches for assessing off-target cleavage.

Thermal profiling in combination with quantitative proteomics makes it possible to identify cellular membrane protein targets of small molecules.

Two approaches for robust, objective segmentation of single-molecule localization data promise better quantitative insights into protein clustering from super-resolution imaging methods.

Applications of focused ion beams for biological ultrastructure and chemical imaging, with a particular emphasis on the coupling of this technology with scanning electron microscopy, are discussed in this Review.

The deep learning–based method DeepSEA predicts the molecular function of noncoding DNA polymorphisms.

By removing modified nucleotides that block reverse transcriptase, two methods have now made tRNAs amenable to RNA-seq.

An integrated single-molecule fluorescence approach enables the study of nano-to-millisecond protein conformational dynamics in living mammalian cells.

Portions of the CRISPR-Cas9 guide RNA can be reworked to carry functional RNA cargos to genomic loci.

International Cancer Genome Consortium members review and recommend approaches to pathway and network analysis to uncover molecular processes that contribute to tumor biology.

Correlated light and electron microscopy (CLEM) gives context to biomolecules studied with fluorescence microscopy. This Review discusses recent improvements and guides readers on probes, instrumentation and sample preparation to implement CLEM.

Antibody-targeted positron emission tomography (immunoPET) can visualize sites of HIV replication that are difficult to access by traditional means such as tissue biopsies.

Nanopore sequencing gets a boost with accurate error modeling and variant-calling tools for Oxford Nanopore Technology's highly anticipated MinION platform.

Two methods for early tagging of sample RNA before RT-qPCR or full RNA-seq open the door to experiments with fewer technical batch effects.

In this Perspective, the authors advance a view of macromolecules as collections ofinterchanging structural ensembles, and discuss how a synergistic combination of NMR,X-ray crystallography, and computational simulations can reveal the structural basis for conformational dynamics at atomic resolution.

A computational method predicts epigenomic marks and DNA methylation patterns from DNA sequence.

A Perspective on the open-source and open-development software project Bioconductor provides an overview for prospective users and developers dealing with high-throughput data in genomics and molecular biology.