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This first of two review articles provides an overview and practical introduction to the precise and accurate localization of single emitters for single-particle tracking and super-resolution localization microscopy.
A new family of genetically encoded ratiometric calcium indicators optimized for imaging of calcium signals in vivo exhibits near-linear fluorescence dynamics while minimizing artifacts caused by movement.
Optofluidic biolasers are emerging as a highly sensitive way to measure changes in biological molecules. Biolasers, which incorporate biological material into the gain medium and contain an optical cavity in a fluidic environment, take advantage of the amplification that occurs during laser generation to quantify tiny changes in biological processes in the gain medium. This Perspective describes the principle of the optofluidic biolaser, reviews recent progress and provides outlooks on potential applications and directions for developing this enabling technology.
