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The combination of computational protein design and single-site saturation mutagenesis enables engineering of allosteric transcription factors to respond to new small molecules.
An inverted light-sheet microscope enables imaging of mouse embryos from zygote to blastocyst with minimal photodamage and high resolution for automatic lineage tree reconstruction, allowing new insight into cell fate specification.
RiboTaper quantifies the three-nucleotide periodicity in Ribo-seq data to find translated open reading frames (ORFs). The de novo inferred set of ORFs comprehensively defines the cellular proteome across a wide expression range and comprises few additional translated noncoding regions.
Heterologous TRP channels can be used to stimulate or ablate neurons in response to their chemical or thermal agonists in zebrafish larvae, providing a set of tools orthogonal to optogenetic manipulation.
Optomechanical actuator nanoparticles collapse upon illumination with near-infrared light. Appropriately coated, they can be used to mechanically trigger cellular processes such as focal adhesion formation or T cell activation.
Measuring the forces generated by cells is not trivial in materials that behave in a nonlinear fashion. An equation that captures this behavior and finite-element modeling can be used to derive these forces from the material deformations around cells.
A concentric-flow microfluidic electrokinetic sample injector enables efficient delivery of microcrystals in their mother liquor for serial femtosecond X-ray crystallography with minimal sample consumption.
Addition of a glycan precursor to cells results in the synthesis and secretion of a large variety of O-glycans that can be purified and biochemically analyzed to profile the cellular O-glycome.
NeuroGPS-Tree can reconstruct individual neurons from dense populations of fluorescently labeled neurons using computational strategies inspired by the strategies humans use.
Pooling barcoded 3C libraries and simultaneously capturing interactions at many loci of interest generates reproducible cis- and trans-interaction maps at high resolution from low amounts of input material. This allows for the comparison of interactions in different cell types using common software designed for differential analysis of sequence count data, rather than requiring software specifically designed for 3C experiments.
Parmbsc1, a new force field for DNA simulations, was broadly tested on nearly 100 DNA systems and overcame simulation artifacts that affected previous force fields.
The Fixed and Recovered Intact Single-cell RNA (FRISCR) method enables robust RNA extraction and sequencing from fixed, stained and sorted single cells and allows unprecedented profiling of rare cell types, including two subpopulations of radial glial cells in the developing human cortex.
Combining a reversibly photoswitchable bacteriophytochrome with photoacoustic imaging allows for exceptionally sensitive tomographic imaging deep in living mice, as well as super-resolution photoacoustic microscopy.
Handling and quantitative image analysis of layered tissues is greatly simplified by cartography with the Image Surface Analysis Environment (ImSAnE), as demonstrated on a variety of specimens, including a beating heart.
A fusion of RNA-binding proteins (RBPs) to a poly(U) polymerase allows the tagging of endogenous RNAs bound by the RBPs with a U-tail that can be used to identify the bound RNA by sequencing. RNA tagging is suited to discover RNA-protein networks in vivo.
A method for profiling changes in membrane protein thermal stability upon ligand binding using mass spectrometry identifies cellular membrane protein targets of small molecules.
A detailed study of the effects of dCas9-KRAB-sgRNA complexes on enhancer activity, gene expression and heterochromatin formation shows high efficacy and specificity.