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Three new red-excitable monomeric fluorescent proteins obtained by structure-guided mutagenesis of mNeptune are described in this work. The authors show the use of one of them, mCardinal, to visualize the differentiation of myoblasts into myocytes in living mice.
Biomolecular interactions are directly detected and visualized in solution with a single-molecule method that measures time-dependent diffusion coefficient and mobility of electrokinetically trapped species.
CS-Rosetta-RNA is a web tool for determining high-resolution structures of noncoding RNA motifs using 1H NMR chemical shift data coupled with Rosetta-based modeling.
The combination of stimulated Raman scattering (SRS) microscopy with an alkyne group as a Raman-active tag allows high-contrast, live-cell dynamic imaging of a variety of biomolecules including DNA, RNA, protein, lipids and small-molecule drugs.
Single-molecule structural transitions involving DNA twisting can be measured with substantially greater spatiotemporal resolution than previously possible with a gold rotor bead tracking (AuRBT) method. This approach uses magnetic tweezers and evanescent darkfield microscopy to track a gold nanoparticle probe attached to a DNA molecule.
This paper reports conditions for the in vitro culture of human leukemia stem cells, which should enable basic studies as well as chemical screens on these cells.
A comparative analysis of methods for scoring human sleep data, in particular sleep spindles, from encephalographic recordings is reported. The authors develop methods for crowdsourcing the identification of sleep spindles and compare the detection performance of experts, non-experts and automated algorithms.
Multivariate linear mixed models implemented in the GEMMA software package add speed, power and the ability to test for genome-wide associations between genetic polymorphisms and multiple correlated phenotypes.
Cryo-scanning transmission electron tomography (CSTET) of unstained, fully hydrated vitrified biological specimens is shown to have advantages over cryo-electron tomography (CET), notably at high sample tilts providing greater depth resolution for thick samples.
High-throughput in vitro analysis of TALE nuclease (TALEN) cleavage specificity gives insight into the mechanism of TALE binding and allows the design of TALENs with lower off-target activity.
The synthetic firefly luciferase substrate CycLuc1 offers brighter bioluminescence and improved imaging in mouse models at lower doses than the standard D-luciferin.
Sequencing the transcriptomes of more than 100 species of alga yields new channelrhodopsins with promising properties for optogenetics. A far red–shifted channelrhodopsin, Chrimson, opens up new behavioral capabilities in Drosophila, and alongside a fast yet light-sensitive blue channelrhodopsin, Chronos, enables independent excitation of two neuronal populations in brain slices.
Four types of mass spectrometry (MS) data—label-free quantitative bottom-up proteomics, native MS, ion mobility–MS and cross-linking MS—are used to derive restraints for structural modeling of large protein assemblies.
The genome-wide annotation of variants (GWAVA) software predicts whether noncoding variants are likely to be functional using a classifier trained on a range of genomic and epigenomic annotations.