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The combination of an engineered demthylase and a highly processive reverse transcriptase during tRNA library preparation for high-throughput sequencing allows comprehensive profiling of the small RNAs.
Temporally stochastic STED nanoscopy with electro-optical deflector–based scanning allows ultrafast super-resolution imaging. The method was used to monitor vesicles and viruses moving at ~2 μm/s with no motion blur.
A miniaturized ultrasound probe enables functional brain imaging in freely behaving rats. The large field of view and deep penetration makes this technique complementary to optical imaging approaches.
Single-particle dynamics are analyzed with hidden Markov modeling in combination with Bayesian model selection. This method can annotate both diffusive and directed motion states with single-step resolution.
The photoactivatable calcium sensors reported in this paper allow simultaneous highlighting of cellular morphology and recording of calcium activity, which is demonstrated in neuronal cultures, in Drosophila and in zebrafish.
Assessing the activity of >1,000 single guide RNAs (sgRNAs) on >1,000 genetic loci with two Cas9 orthologs provides insight into sgRNA design and epigenetic parameters for optimal activity.
A force-distance curve–based atomic force microscopy method is developed and applied to both image and quantify the ligand-binding free-energy landscape of single protease-activated receptor-1 (PAR1) molecules under physiologically relevant conditions.
Image reconstruction by integrating exchangeable single-molecule localization (IRIS) allows for super-resolution imaging of multiple targets. IRIS yields continuously labeled structures owing to high labeling density and is demonstrated for multiple cytoskeletal components.
Individual protein dynamics can be studied in live eukaryotic cells from the millisecond to nanosecond level using an integrated approach to confocal single-molecule FRET spectroscopy.
A quantitative mass spectrometry–based standard operating procedure to classify antibody performance in immunoprecipitation is assessed in a multilaboratory study.
Unique mutational signatures induced by cross-linking of m6A-specific antibodies to RNA identify m6A and m6Am residues at single-nucleotide resolution, transcriptome-wide.
A hybrid method that combines sparse NMR spectroscopy data with evolutionary residue-residue coupling information is used to solve accurate structures of large proteins.
A combination of single-molecule long-read sequencing, single-molecule genome mapping and short-read sequencing provides reference-quality de novo assemblies and also shows improved phasing and variant detection over short-read assemblies when mapping to a reference genome.
This paper reports magnetic imaging of immunolabeled mammalian cells using nitrogen-vacancy centers in diamond and shows that the method can be used for quantitative profiling of markers.
The combination of immunofluorescence and single-molecule FISH (smFISH) enables a quantitative description of how transcription factor binding drives the kinetics of mRNA production.