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An inverted light-sheet microscope enables imaging of mouse embryos from zygote to blastocyst with minimal photodamage and high resolution for automatic lineage tree reconstruction, allowing new insight into cell fate specification.
Heterologous TRP channels can be used to stimulate or ablate neurons in response to their chemical or thermal agonists in zebrafish larvae, providing a set of tools orthogonal to optogenetic manipulation.
Optomechanical actuator nanoparticles collapse upon illumination with near-infrared light. Appropriately coated, they can be used to mechanically trigger cellular processes such as focal adhesion formation or T cell activation.
A concentric-flow microfluidic electrokinetic sample injector enables efficient delivery of microcrystals in their mother liquor for serial femtosecond X-ray crystallography with minimal sample consumption.
NeuroGPS-Tree can reconstruct individual neurons from dense populations of fluorescently labeled neurons using computational strategies inspired by the strategies humans use.
Parmbsc1, a new force field for DNA simulations, was broadly tested on nearly 100 DNA systems and overcame simulation artifacts that affected previous force fields.
Handling and quantitative image analysis of layered tissues is greatly simplified by cartography with the Image Surface Analysis Environment (ImSAnE), as demonstrated on a variety of specimens, including a beating heart.
A method for profiling changes in membrane protein thermal stability upon ligand binding using mass spectrometry identifies cellular membrane protein targets of small molecules.
Brillouin microscopy can be used to analyze the mechanical properties of cells in a contact-free fashion. Cells in 2D and 3D environments are accessible to this technology, which provides measurements of longitudinal moduli at optical resolution.
The molecular architecture of protein complexes can be determined using an optimal approach for isolating GFP-tagged complexes at native levels, combined with cross-linking, mass spectrometry analysis, and structure modeling from mass spectrometry-derived distance restraints.
A method for profiling alterations in protein thermal stability after ligand binding using mass spectrometry identifies the cellular protein targets of drugs and metabolites in living cells.
The length of a single guide RNA (gRNA) determines the function of Cas9. In this study 20-nt gRNAs allowed nuclease activity and genome editing, whereas 14-nt gRNAs mediated transcriptional activation or repression.
Tiling of regulatory DNA with mutations introduced by genome editing nucleases and linking the resulting alleles to a phenotypic readout allows the precise determination of functional sequence motifs within these regions.
Cycler constructs a trajectory of cell-cycle progression from fixed images of cells enabling the correlation of an individual cell's position in the cell cycle with multiple cellular readouts.