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Lim et al. developed a fluorescence resonance energy transfer-based assay to identify anti-CRISPR molecules and discovered an SpCas9 inhibitor that is twofold more efficient in inhibiting Cas9 at diverse genomic loci than existing inhibitors and easy to synthesize.
Truong et al. report a system to monitor RNA expression by modifying an intron within a gene of interest. This additional engineered transcript then hijacks nuclear export machinery for subsequent translation of a reporter gene.
Zou et al. use a single multi-target guide RNA to direct Cas9 to a high number of loci mapped by high-throughput short-read sequencing. This multi-target CRISPR system allows detailed studies of genome editing and DNA repair.
Rosebrock, Arora et al. report a method to overcome limited cortical cellular diversity in human organoids, thus mirroring fundamental features of cortical development and offering a basis for organoid-based disease modelling.
John Peter et al. develop METALIC (Mass tagging-Enabled TrAcking of Lipids In Cells), an approach to track interorganelle lipid flux in live cells using organelle-targeted enzymatic labelling of lipid subpopulations and mass spectrometry.
Lummertz da Rocha et al. present CellComm, an algorithm that analyses cell–cell communication to predict signalling and regulatory networks, and identify regulators of haematopoietic development in the aorta–gonad–mesonephros region.
Through structure-guided protein engineering, Guo et al. developed a Lachnospiraceae bacterium Cas12a variant with enhanced editing efficiency and applied the enzyme-dead version of this variant for multiplex gene activation in the mouse retina.
Wang, Qu et al. developed a genome-editing system, utilizing catalytically inactive Cas9 fused to microbial single-strand annealing proteins, for kilobase-scale insertion in human cells without introducing DNA nicks or breaks.
Chowdhury, Sau and Musser report a multicolour imaging approach that enables the 3D visualization of cargo transport trajectories relative to a super-resolved nuclear pore complex scaffold in non-fixed permeabilized cells.