Abstract
The allometry of the pipe model quantifies the approximate proportionality between the tree leaf amount and the stem cross-sectional area at the crown base (ACB). It is useful for estimating and modeling carbon fixation abilities of trees but requires climbing the tree and is thus unsuitable for large-scale studies. Here, we adopted a previously proposed allometry (hereafter the quasi-pipe (qPipe) model allometry) formulating the relationship between the tree leaf amount and a surrogate of ACB, ACB_Est, calculated from tree dimensions measurable from the ground. Using published/unpublished data for 962 trees of 159 species collected between tropical rainforests and boreal forests, we established pipe and qPipe model allometries for evergreen-conifer, deciduous-conifer, evergreen-broadleaf, and deciduous-broadleaf plant functional types (PFTs). For the leaf area per tree (LA), allometric lines on a log–log plane were almost identical among the four PFTs in both models, with slopes of ~ 1. For the leaf mass per tree (LM), however, the allometric lines separated among the four PFTs in both models and had slopes greater than 1, indicating that the proportionality assumed in the pipe model held for LA but not LM. The applicability of the qPipe model in estimating the stand-scale leaf amount was further examined.
Similar content being viewed by others
Introduction
The estimation of the amount of leaves in forests at a global scale is essential in projecting and modeling carbon fixation abilities on Earth1. Remote-sensing technologies, such as satellite observations and light detection and ranging, allow the estimation of the amount of leaves per unit land area, such as the estimation of the leaf area index (LAI, the leaf area per unit land area), over a wide area2. Still, methods of obtaining the ground truth of the LAI via fieldwork-based stand-scale ecological studies are needed to support the remote-sensing technologies3,4. One of the major ground-truth methods is the allometric method5, in which the amount of leaves per tree is estimated using a known relation between the amount of leaves (leaf area or leaf mass) and measures of the tree size such as the trunk diameter at breast height (DBH) and tree height. The LAI of a stand is estimated by summing the allometrically estimated tree leaf area for all trees of a stand and dividing by the stand area. One advantage of the allometric method is that it allows us to calculate the confidence intervals and/or prediction intervals of the total amount of leaves per stand, as these intervals can be calculated from estimates of the prediction intervals of each individual tree via regression calculation6 and the rule of propagation of errors7,8.
However, there are problems in implementing the allometric method. First, when the DBH or the combination of the DBH and tree height are used as the predictor variable(s) of the tree leaf amount, the allometric equation prepared for a stand is in general not applicable to the same stand in the future or to other stands. A typical reason is that the leaf amount of a given tree may not change or even decrease, instead of increasing, despite the DBH and tree height continuing to increase with age8,9. This is one reason why both the intercept and slope coefficients of a linear allometric equation change with stand age10,11,12.
The allometric equation known as the pipe model of the tree shape13,14 is suitable for estimating the leaf amount of a tree. The model predicts the tree leaf amount as being approximately directly proportional to the stem cross-sectional area at the crown base (ACB) or the crown base stem diameter squared (DCB2). As the pipe model uses ACB or DCB2 as the predictor of the leaf amount, it can express a constant or decreasing leaf amount over time; a tree with a decreasing leaf amount generally has a decreasing crown length because the speed of the rise of crown base location exceeds the height growth owing to the death of lower branches8,9. Such a rapid crown-base rise often leads to a decrease in ACB and the leaf amount8,9.
After the publication of the pipe model, many studies confirmed the validity of the model and related it to the relationship between the tree leaf amount and stem sapwood area at the crown base15,16. In this paper, however, we refer to the pipe model as the approximate proportional relationship of the tree leaf amount with the stem cross-sectional area at the crown base ACB or DCB2 and not the relationship with the sapwood area of a trunk as in other studies. The validity of the pipe model implies that the ‘pipe model ratio’17 (defined as the leaf amount per unit area of the stem cross section or unit sapwood area) is constant irrespective of the tree size. Meanwhile, little attention has been paid to whether the amount of leaves in the pipe model should be expressed on a leaf mass basis or a leaf area basis16. The leaf amount in the original pipe model13 was expressed on a leaf mass basis, whereas some later studies expressed the leaf amount on a leaf area basis. If the pipe model ratio is constant for both the tree leaf area and tree leaf mass, the tree leaf mass per tree leaf area (tree LMA) must be constant between trees of different size in a stand. However, studies of an individual-leaf scale suggest that the LMA of an individual leaf is affected by the light conditions such that it decreases with decreasing light from the canopy surface downward18,19,20,21. It is thus expected that the tree LMA is smaller for shorter trees in a stand than for taller trees in the same stand. If so, the pipe model ratio being constant between trees cannot hold for both the leaf area and leaf mass simultaneously. In other words, the proportionality between ACB or DCB2 and the tree leaf amount cannot hold simultaneously for both the leaf area and leaf mass, unless the tree LMA is constant among trees of different size in a stand. This means that when the allometric line is drawn on a log–log plane, the slope should be different between the allometry for the leaf area and that for the leaf mass.
Care must be taken when estimating the slope of a regression line. In estimating the slope, the use of a regression method called Model II regression is recommended as it calculates reasonable estimates of the slope by considering residuals of both 'x' and 'y' simultaneously6,22. Meanwhile, the regression method used for predicting the 'y' value from a given value ‘x’ is called Model I regression. As the slope of the regression line of Model II regression is known to be greater than that of the Model I regression22, allometric analyses for establishing the relationship (Model II) and for prediction (Model I) should be conducted simultaneously.
Another practical problem of the pipe model is that one needs to climb to the base of the tree crown to measure ACB. This issue has partly been solved adopting a method for approximating ACB without the need for climbing12. The following relationship was obtained from measurements for 156 trees of Betula ermanii Cham. in three stands12:
where ABH is the stem cross-sectional area at a 1.3-m height, LC is the crown length (i.e., the difference between the tree height and height of the crown base), and H>1.3 the length of the stem part above a height of 1.3 m. This is equivalent to assuming that there is a virtual paraboloid of revolution that has its apex at the treetop, and cross-sectional areas at the crown base (i.e., at the length LC from the treetop) and at the breast height being ACB and ABH, respectively. It follows from Eq. (1) that ACB can be approximated as12
where ACB_Est is an estimate of the stem cross-sectional area at the crown base, and RCROWN is the crown ratio defined as LC/H>1.3. As ABH, LC, and H>1.3 are all measurable from the forest floor, the use of ACB_Est as the surrogate of ACB is practical in estimating the tree leaf amount just like for the pipe model.
So far, the application of Eq. (2) has only been reported for two species12,23. Both studies showed that the actual measured ACB and its estimate ACB_Est were not statistically different, such that ACB and ACB_Est were treated as though they were convertible predictors of the leaf amount. The allometry for the tree leaf area using ACB_Est as a predictor variable was found to be not statistically different from the pipe model allometry using ACB as a predictor variable12. When estimating the LAI of the stand, the tree leaf area for trees whose ACB could not be directly measured in the cited study was estimated by substituting ACB_Est into an existing pipe model allometric equation, namely the ACB–tree leaf area relationship12. In contrast, the present study presumes that the pipe model allometry is unavailable owing to a lack of ACB data, and this is why the allometry that uses ACB_Est as a predictor variable is needed. Therefore, we deal with the allometry using ACB_Est and that using ACB (i.e., the pipe model allometry) differently in the present study. Hereafter, the allometry using ACB_Est as a predictor variable for the tree leaf amount12 is referred to as the quasi-pipe (qPipe) model allometry to indicate the difference. The form of the allometric equations in the present study is given in the caption of Table 1. A comparison between the pipe and qPipe model allometries is presented as equations (S1) and (S5) in Supplementary Information S1 and Supplementary Fig. S1.
The reason why Eq. (2) holds for estimating ACB is not biologically based but is simply based on a general characteristic that the tree trunk tapers toward the treetop (see the Discussion). As Eq. (2) does not require the determination of regression coefficients such as the intercept and slope, if Eq. (2) is found to hold for other species, the qPipe model would be a convenient tool for estimating the tree leaf amount when no allometric relationship is available for a particular stand.
To test the applicability of the qPipe model to other species at a global scale, plant functional types (PFTs) [i.e., the combination of evergreen/deciduous (E or D) and broadleaved/coniferous (B or C)], would be useful, as PFTs are identifiable through remote sensing and are related to ecosystem-scale physiological functioning24,25. Hereafter, the four PFTs are abbreviated as EB, EC, DB, and DC (excluding monocods such as palm-tree species).
In this study, we propose the qPipe model allometry for estimating the stand-scale leaf biomass and LAI in large-scale studies, by pooling the data of the tree species of each PFT. Published/unpublished data for 962 trees collected from tropical rainforests in Southeast Asia, temperate forests of Japan and Tasmania, and boreal forests of Japan and Siberia are used in analyses. The number of trees (species) is 453 (102), 225 (10), 227 (44), and 57 (3) for EB, EC, DB, and DC PFTs, respectively. The list of species and number of trees are given in Supplementary Information S3. Hereafter, the term ‘leaf mass’ refers to the leaf dry mass. The leaf area for broadleaf species is the one-sided area of a leaf. For conifer species, the leaf area in all data used in the analyses is measured as the projected area by detaching all needles from the stem. We first examine (1) whether the relationship between ACB and ACB_EST is 1:1 using standardized major axis (SMA) regression (the smatr library22 in R26), which is Model II regression suitable for estimating the slope6. Then (2) for the four PFTs, the pipe model allometries are explored and compared with qPipe models through SMA regression. Next, (3) to estimate the leaf area and leaf mass and their 95% prediction intervals using Model I regression, the qPipe models are formulated via general linear mixed models (GLMMs), with species being designated as a random effect. This regression method is a Model I regression6. In both SMA regression and GLMMs, best models are selected using the Akaike information criteria (AIC). Lastly, (4) to present an example of how LAI estimates for one stand differ between the pipe and qPipe model allometries, a comparison is made between LAI estimates using published 20-year changes (from 21 to 40 years of stand age) in the LAI measured in an evergreen conifer ‘hinoki’ cypress (Chamaecyparis obtusa (Siebold et Zucc.) Endl.) stand8. Using tree census data including data of the crown base stem diameter recorded for each tree each year over the 20 years9, the LAIs of each year and their 95% prediction intervals are calculated by adopting three methods; i.e., adopting the pipe model allometry prepared for the stand8 using the measured ACB as the predictor (‘site-specific pipe model’), the qPipe model allometry prepared using ACB_Est as the predictor, where ACB_Est is calculated using the same tree census data (‘site-specific qPipe model’; see Supplementary Information S1), and the qPipe model allometry of the PFT obtained in (3) using ACB_Est (‘global qPipe model’). In this comparison, the 95% prediction intervals of each LAI estimates are calculated through an error propagation of 95% prediction intervals of the leaf area estimates of individual trees7,8. All statistical calculations are performed using R26.
Results
Comparison between measured A CB and estimates A CB_Est
The measured values of ACB (m2) and the estimates ACB_Est (m2) calculated with Eq. (2) were compared among PFTs using SMA regression (Fig. 1). Both ACB and ACB_Est were log-transformed in the regression calculation. The best model was the one having ln(ACB) and the four PFTs (categorical variable) as the predictor variables (Table 1). The allometric line was drawn for each PFT. Figure 1 shows that the relationship was almost 1:1 for each PFT. The slopes of the four PFTs ranged between 0.99 and 1.01, without significant differences from 1.0 (Table 1). Pair-wise differences were significant between the elevations (intercepts) of the regression lines of the four PFTs except between the elevations of the EC and DC PFTs and between the elevations of the EB and DB PFTs. However, these differences were small as illustrated in Fig. 1, in which the four regression lines almost overlap. This result suggests that ACB_EST can be used practically as a surrogate of ACB for each PFT.
Comparison between the pipe- and the qPipe-model allometries using Model II (SMA) regression
For the pipe model relationship using SMA regression, the model having ln(ACB) and PFTs as the predictor variables was selected with the natural-log transformed leaf area (Fig. 2a) and leaf mass (Fig. 2b) as the response variables.
For the leaf area (Fig. 2a), the slopes of allometric lines were not significantly different between the four PFTs (likelihood ratio test, P = 0.053), ranging between 1.15 and 1.01 (Table 1). The slopes of broadleaf PFTs (EB, DB) were not significantly different from 1, indicating that the pipe model ratio for the leaf area was constant and the slope follows the pipe model presumption in broadleaf PFTs. In contrast, for conifer PFTs, the difference from 1 was significant in the EC PFT (slope of 1.09, Table 1). The DC PFT had the largest slope (1.15) but the difference from 1 was not significant, probably because only 15 leaf area data were available for the DC PFT, all for Japanese larch (Larix kaempferi (Lamb.) Carr.) trees. Slopes of the conifer PFTs suggest that their pipe model ratio tended to increase with increasing tree size. Pair-wise comparisons among PFTs showed that the difference between PFTs in the elevation of the allometric line was significant only between DC and EC PFTs (Table 1). Despite these differences, regression lines of the four PFTs almost overlapped (Fig. 2a), indicating that pipe model allometries for leaf area were similar across PFTs.
In contrast, for the leaf mass, the separation of allometric lines was distinct among the four PFTs (Fig. 2b). Differences were significant in both slopes (likelihood ratio test, P = 0.018) and intercepts (P < 0.000). Elevations of regression lines were greater for conifer (EC, DC) PFTs than for broadleaf (EB, DB) PFTs and were greater for evergreen (EC, EB) PFTs than for deciduous (DC, DB) PFTs [likelihood ratio test for multiple comparison, all P < 0.000; Table 1 (2b)]. Furthermore, the slopes tended to be greater for conifer (EC, DC) PFTs than for broadleaf (EB, DB) PFTs (Table 1 (2b)). More importantly, all four PFTs had significant slopes greater than 1, indicating that the pipe model ratio for the leaf mass differs by tree size, with larger trees having a greater pipe model ratio.
The qPipe models that used ln(ACB_EST) and PFTs as the predictors (Fig. 2c, d) gave results similar to the pipe model (i.e., Fig. 2a, b) with a few exceptions: for leaf area (Fig. 2c), the difference from 1 in the slope was not significant except for the EC PFT (Table 1(2c)). Although differences in elevation were significant between EC and DB PFTs (likelihood ratio test for multiple comparison, P < 0.000) and between EC and DC PFTs (P = 0.027), the four allometric lines overlapped around the center of the data distribution (Fig. 2c) as in the case of the ACB–leaf area relationship (Fig. 2a).
For the leaf mass, the qPipe model had clear differences in regression lines (Fig. 2d), as in the case of the pipe model (Fig. 2b). Elevations were significantly different among PFTs (likelihood ratio test, P < 0.000) with conifer PFTs (EC, DC) having higher elevations than broadleaf (EB, DB) PFTs (likelihood ratio test for multiple comparison, P < 0.000) as in the pipe model (Table 1(2d)). Additionally, the slopes tended to be greater for conifer (EC, DC) PFTs than for broadleaf (EB, DB) PFTs and were significantly different from 1 except for the DB PFT (Table 1(2d)).
In summary, for the leaf area, both the pipe model and qPipe gave regression slopes closer to 1, with little apparent difference in the allometric line among PFTs. For the leaf mass, in contrast, slopes of the four PFTs were greater than 1 in both the pipe model and the qPipe model, and their allometric lines tended to differ in elevation.
Prediction of the tree leaf area using the qPipe model allometry
To predict the tree leaf area, the allometric relationship of the qPipe model was obtained through model selection with GLMMs (Fig. 3b). For comparison, the results of the pipe model using GLMMs (Fig. 3a) are also presented. In both cases, the PFT was not selected as a predictor of the Model I regression, in contrast with the results of Model II regression (Fig. 2). Selected models were those with ln(ACB) or ln(ACB_EST) as the fixed effect variable and species as the random intercept term (Fig. 3a, b, Table 1). This indicates that, for predicting leaf area using ACB or ACB_EST, differences in the PFT are negligible as far as our collective data sets are concerned. In other words, the qPipe model (Fig. 3b) is applicable to the prediction of the leaf area of a tree without the need to consider differences in the PFT. Note, however, that different species would have different elevations of the allometric line as the species was selected as the random effect on the elevation. Ninety-five percent prediction intervals are drawn in Fig. 3 using an approximating equation, which is given in Table 2 (see also Supplementary Information S2). The intervals are seen to be slightly wider for the qPipe model (Fig. 3b) than for the pipe model (Fig. 3a).
Prediction of the tree leaf mass using the qPipe models
The results presented in Fig. 2b, d clearly show that, in predicting the tree leaf mass, the four PFTs have different qPipe model allometry equations. Hence, the regression calculation was performed for each PFT independently, allowing the calculation of prediction intervals. Models with species as the random intercept term were selected for the EC, EB, and DB PFTs (Fig. 4a, c, d) whereas ordinary least-squares (LS) linear regression without a random effect was selected for the DC PFT (Fig. 4b, Table 1). Consistent with the results of Model II regression (Fig. 2d), the elevation of the allometric line tended to be greater for the evergreen trees than for the deciduous trees in both conifer and broadleaf PFTs (Table 1).
Example of the comparison of the pipe and qPipe models for the LAI
Lastly, the three methods of stand LAI estimation were compared. A 20-year change in the LAI of an evergreen conifer hinoki (C. obtusa) stand was estimated using the measured ACB of each tree and the original8 site-specific pipe model allometry prepared for the particular stand, the calculated ACB_Est and the site-specific qPipe model allometry, and the calculated ACB_Est of each tree and the global qPipe model allometry for the leaf area (shown in Fig. 3b). The LAI estimated with the site-specific qPipe model showed interannual changes similar to those of the LAI estimated using the site-specific pipe model, though there were differences (but not significant) in the LAI (Fig. 5a). A positive relationship between the LAI and 6-year running average of the mean summer temperature, which has been reported previously8, was reproduced with the site-specific qPipe allometry (Fig. 5b). However, the LAI estimated with the global qPipe allometry could not reproduce the relationship (Fig. 5a, b), especially at earlier stand ages. These results demonstrate that qPipe allometry is useful if the site-specific qPipe allometry is available together with measurements of the tree height, crown-base height, and DBH for each tree, whereas the global qPipe allometry may need to be carefully considered when applied to the LAI estimation of an unknown stand, as we discuss later.
Discussion
Leaf area versus leaf mass
Although the main objective of the present study is to examine the applicability of the qPipe model, the study also advances our understanding of the pipe model. An important presumption of the original pipe model is that the amount of leaves per unit cross-sectional area of the trunk at the crown base, or the pipe model ratio, is constant13. We show that this appears to hold for the leaf area but not for the leaf mass, as long as the stem cross-sectional area ACB is considered as a predictor variable. In the Model II regression analysis (Fig. 2a, Table 1), the slope of approximately 1 for PFTs except for the EC PFT indicates that the pipe model ratio for the leaf area (leaf area per unit ACB) was statistically constant irrespective of ACB. In contrast, for the leaf mass, the slope of the Model II regression was significantly greater than 1 for each PFT (Fig. 2b, Table 1). This indicates that the pipe model ratio for the leaf mass (leaf mass per unit ACB) increased with increasing ACB. The difference in the slope of a PFT between the leaf area and leaf mass can be ascribed to a tendency that taller trees tend to have a greater tree LMA than shorter trees, as mentioned in the Introduction.
Figure 2a also shows an interesting result for the leaf area that there was little difference in the pipe model allometries, if at all, between PFTs. This implies that the pipe model ratio for the leaf area remained approximately constant irrespective of the PFT as long as ACB is used as the denominator of the pipe model ratio. However, the use of the sapwood area in the stem cross section, rather than the stem cross-sectional area ACB, has been regarded as a reason why the pipe model holds, as the former can biologically explain the hydraulic functioning15,16. Additionally, the xylem structure for the water transport system is known to vary between the four PFTs27,28. For example, the gymnosperm (EC and DC) species depend on tracheids for water transport and do not have vessels, unlike angiosperm (EA and DA) species. Even between angiosperm species, the vessel structure of the xylem on the stem cross-section varies; e.g., between ring-porous wood (e.g., some deciduous oak spp.), diffuse-porous wood (some beech spp.), and radial-porous wood (some evergreen oak spp.). Considering these possible variations in xylem hydraulic properties, it seems curious that the slope of approximately 1 held for the leaf area–ACB relationship with almost no difference among the four PFTs. We do not have an explanation for this as yet. Considering that the leaf area rather than the leaf mass is likely the primary quantity of leaves relating to photosynthesis20,29, trees may adjust to maximize water conduction efficiency for photosynthesis and transpiration irrespective of the xylem structure30, which may have resulted in a convergence to a similar proportionality among PFTs in the leaf area–ACB relationship.
For the pipe model relationship to hold, stem thickening growth must occur in such a way that the stem cross-sectional area or sapwood area is coordinated to be proportional to the leaf amount. However, it is not known what types of signal are involved in this coordination. One possibility would be the effect of the plant hormone auxin (indole-3-acetic acid, IAA), which plays an important role in determining the radial growth of a stem by affecting the activity of cell division in the cambial meristem31. As IAA is produced in apical buds and growing shoots in the crown and transported to the cambial region of the stem, the IAA level in the cambial region is known to be affected by the crown size32,33,34,35. This may be a cue to the connection between the leaf amount and the stem cross-sectional area. Meanwhile, a twig-scale study suggested a mechanism that allows the pipe model relationship to hold in terms of the source–sink relationship36. Further study combining several biological mechanisms may be necessary to solve the abovementioned questions.
How and when does the qPipe model work?
Figures 1 and 2 show that the qPipe model provided allometry comparable to that obtained using the pipe model. However, whether the qPipe model can fairly estimate the actual ACB or not depends on the actual form of the tree stem. Here, we explain this using the stem taper equation, a mathematical expression of the change in stem thickness from breast height to treetop. In the field of forest sciences, the shape of a tree stem is often expressed by a simple equation37. The relative taper of the stem between the crown base and breast height is expressed as
where L is the distance from the treetop along the stem (relative scale; 0 < L ≤ 1, where L = 1 at breast height), A is the stem cross-sectional area at L (relative scale; 0 < A ≤ 1, where A = 1 at breast height), and X is a scaling parameter indicating the degree of stem taper. The stem shapes of a cone, paraboloid of revolution, and cubic solid of revolution correspond to X = 1, 2, and 3, respectively (curved lines in Fig. 6a). As mentioned in the Introduction, the calculation of ACB_Est in the qPipe model is based on the assumption of a paraboloid of revolution; i.e., X = 2 in Eq. (3). It follows that, if the qPipe model is applied to a tree stem having X < 2, then ACB_Est > ACB (i.e., an overestimation, as L2/2 > L2/X), and if applied to trees having X > 2, then ACB_Est < ACB (i.e., an underestimation, as L2/2 < L2/X) (Fig. 6a). Hence, concordance between ACB and ACB_Est (Fig. 1) depends on the stem shape that a tree actually had, or the X value in Eq. (3). This is a possible source of estimation error of ACB_Est for an individual tree. However, in estimating the total leaf amount of a stand, what should be considered further is the balance between overestimation and underestimation among the trees in the stand. The stand-level leaf amount may be overestimated if there are many trees with X < 2 and underestimated if there are many trees with X > 2.
The data points of Fig. 6a illustrate an example of actually measured ACB for all trees in an even-aged evergreen conifer stand at 21 and 40 years of stand age8,9. At 21 years of stand age, a majority of the trees had A (≡ ACB/ABH) at the crown base in the region X < 2 (Fig. 6a), indicating that their ACB_Est was an overestimation of the actual ACB (Fig. 6b). This tendency changed at 40 years of stand age, when there were still some overestimated trees but many underestimated trees also (Fig. 6a, b). This change is ascribed to a change in the stem taper with tree growth under competition. The good estimation of the LAI by the site-specific qPipe model with non-significant differences in the LAI (Fig. 5a) was partly due to the overestimation and underestimation offsetting each other between trees. It is likely that trees of a stand vary in terms of X in Eq. (3).
A study of four conifer species in North America37 reported that there were more trees with X = 2 than those with X = 3. This may suggest that the assumption of X = 2 in the qPipe model is likely a moderate assumption for estimating the stand LAI due to the presence of trees with both X < 2 and X > 2 in a stand. Additionally, the qPipe model provides over/underestimations due to other factors. For example, many long-lived ‘giant’ trees38,39 are known to undergo treetop breakage because of natural phenomena such as lightning strikes and strong winds. Such trees are expected to have a less tapered, cylindrical stem (i.e., X > 2 in Eq. (3)) owing to the loss of their top part and would have a much higher H value if breakage had not occurred. The leaf amount for such top-broken trees may result in underestimation with the qPipe model. Another situation that may lead to error is a species having leaf turnover without turning over branches; evergreen conifers such as black spruce (Picea mariana (Mill.) B.S.P.) turnover needled shoots with epicormic branching (re-sprouting of new shoots) on a primary branch whereas older shoots on the same branch wither and fall40,41. For such a tree, the amount of leaves per tree may be in a steady state, whereas ACB may increase annually without a change in its crown-base location. If this happens, the leaf amount per unit ACB should decrease with age. These problems apply to not only the qPipe model but also the pipe model. The applicability of the qPipe model and pipe model needs to be carefully considered for trees having an atypical crown structure.
Conclusion
With our collective dataset, the constant pipe model ratio assumed in the pipe model held for LA but not for LM, which likely reflects changes in the tree LMA with tree size. We proposed qPipe model allometry considering the difference in PFTs, where measurements of the tree height, crown-base height, and DBH are used in estimating the leaf area and leaf mass of an individual tree. As these measurements can be made in the field and the 95% confidence intervals of the estimated individual leaf area or mass are calculable, the stand LAI and its confidence intervals can be estimated for a stand for which the stand-specific allometry of the leaf area or leaf mass is not available, as is often the case in large-scale studies. Although data for ascertaining the applicability of the qPipe model to LAI estimation are still limited, we believe that the investigation of the applicability of the present method is worth continuing in future work.
Materials and methods
Data sources
Many of the data of the present study are taken from the BAAD dataset42, which is a meta-dataset comprising many published/unpublished data of individual trees. However, while choosing data suitable for the present study, we found that a part of the BAAD data had been incorrectly copied from original data. We referred to the original data source in adding/correcting such data. Additionally, other data sources were collected and combined with the BAAD data.
With the combined data, we selected individual trees that satisfied the conditions of a tree height greater than 1.3 m, breast height of at least 1.3 m, crown-base height of at least breast height, either the stem diameter or cross-sectional area being recorded for both the breast height and crown-base height, and either the leaf area (LA, m2) or leaf dry mass (LM, kg) being recorded for an individual tree. In the process of regression calculation, we carried out Grubb’s test (R package “outliers”43) to remove outliers from each of the ln(ACB)–ln(LA) and ln(ACB)–ln(LM) relationships until no outliers remained. If necessary, Grubbs’ test was conducted for each PFT or each species. Data for palm trees (monocots) were excluded. The list of all of the data used in the analyses of the present study is given as the Supplementary file “AllData.csv”, the explanation of which is given in Supplementary Information S4.
Data analyses
All statistical calculations were made using R26. For Model II regression (Figs. 1, 2), the SMA regression function available from the package smatr44 in R was used, where the “robust” setting was adopted to lessen the effects of outliers. Model selection was performed using the AIC. As the current version of smatr does not allow the calculation of random effects, PFTs were treated as the predictor by pooling all species in each PFT. In Model I regression used for predictions (Figs. 3, 4), ordinary least-squares or the mixed models package lme445 was used, both of which allow the calculation of prediction intervals. In GLMMs with log-transformed variables, a Gaussian error distribution was chosen for normality. Models with the PFT as either a fixed effect or random effect were compared to select the best models using the AIC. The species in each PFT were treated as a random effect, where model selection was made by comparing models including species only in a random-effect intercept term with those including species in both intercept and slope terms. Some models with error messages (e.g., the model failed to converge) were discarded irrespective of the AIC. As a result, in all mixed models of the present study (Figs. 4, 5a, c, d), the species were treated as the only intercept term of the random effect.
Note that the prediction intervals for the mixed models in Figs. 3 and 4 were first calculated with lme4. The prediction intervals were then approximated with the quadratic equation function of ACB or ACB_Est (Table 2) to allow the calculation of prediction intervals for other studies. This approximation was performed using the non-linear regression method nls() in R. An example of the results of calculation is given in Supplementary Fig. S2.
As the regression calculation for Model I regression (Figs. 3, 4) was made through natural-log transforming the predictor and the responses of a power function, the bias arising from the log-transformation was corrected by multiplying the power function with a correction factor CF12,46. The CF values are given in Table 1. See also equation (S5) in Supplementary Information S1 for an example of the equation.
Data availability
The data used in the present study is available as Supplementary Information File S1.
References
Gower, S. T. Patterns and mechanisms of the forest carbon cycle. Annu. Rev. Environ. Resour. 28, 169–204 (2003).
Parker, G. G. Tamm review: Leaf Area Index (LAI) is both a determinant and a consequence of important processes in vegetation canopies. For. Ecol. Manag. 477, 118496 (2020).
Cleveland, C. C. et al. A comparison of plot-based satellite and Earth system model estimates of tropical forest net primary production. Glob. Biogeochem. Cycles 29, 626–644 (2015).
Popkin, G. Weighing the world’s trees. Nature 523, 20–22 (2015).
Gower, S. T., Kucharik, C. J. & Norman, J. M. Direct and indirect estimation of leaf area index, fAPAR, and net primary production of terrestrial ecosystems. Remote Sens. Environ. 70, 29–51 (1999).
Sokal, R. R. & Rohlf, F. J. Biometry 3rd edn. (Freeman, 1995).
Taylor, J. R. An introduction to error analysis 2nd edn. (University Science Books, 1997).
Sumida, A., Watanabe, T. & Miyaura, T. Interannual variability of leaf area index of an evergreen conifer stand was affected by carry-over effects from recent climate conditions. Sci. Rep. 8, 13590 (2018).
Sumida, A., Miyaura, T. & Torii, H. Relationships of tree height and diameter at breast height revisited: analyses of stem growth using 20-year data of an even-aged Chamaecyparis obtusa stand. Tree Physiol. 33, 106–118 (2013).
Kira, T. & Shidei, T. Primary production and turnover of organic matter in different forest ecosystems of the Western Pacific. Jpn. J. Ecol. 17, 70–87 (1967).
Holdaway, R. J., Allen, R. B., Clinton, P. W., Davis, M. R. & Coomes, D. A. Intraspecific changes in forest canopy allometries during self-thinning. Funct. Ecol. 22, 460–469 (2008).
Sumida, A. et al. Ground-based estimation of leaf area index and vertical distribution of leaf area density in a Betula ermanii forest. Silva Fenn. 43, 799–816 (2009).
Shinozaki, K., Yoda, K., Hozumi, K. & Kira, T. A quantitative analysis of plant form–the pipe model theory I. Basic analysis. Jpn. J. Ecol. 14, 97–105 (1964).
Shinozaki, K., Yoda, K., Hozumi, K. & Kira, T. A quantitative analysis of plant form–the pipe model theory II. Further evidence of the theory and its application in forest ecology. Jpn. J. Ecol. 14, 133–139 (1964).
Grier, C. C. & Waring, R. H. Conifer foliage mass related to sapwood area. For. Sci. 20, 205–206 (1974).
Lehnebach, R., Beyer, R., Letort, V. & Heuret, P. The pipe model theory half a century on: A review. Ann. Bot. 121, 773–795 (2018).
Berninger, F. et al. Effects of tree size and position on pipe model ratios in Scots pine. Can. J. For. Res. 35, 1294–1304 (2005).
Kira, T., Shinozaki, K. & Hozumi, K. Structure of forest canopies as related to their primary productivity. Plant Cell Physiol. 10, 129–142 (1969).
Coble, A. P. & Cavaleri, M. A. Light drives vertical gradients of leaf morphology in a sugar maple (Acer saccharum) forest. Tree Physiol. 34, 146–158 (2014).
Poorter, H., Lambers, H. & Evans, J. R. Trait correlation networks: A whole-plant perspective on the recently criticized leaf economic spectrum. New Phytol. 201, 378–282 (2014).
Fajardo, A. & Siefert, A. Temperate rain forest species partition fine-scale gradients in light availability based on their leaf mass per area (LMA). Ann. Bot. 118, 1307–1315 (2016).
Warton, D. I., Wright, I. J., Falster, D. S. & Westoby, M. Bivariate line-fitting methods for allometry. Biol. Rev. 81, 259–291 (2006).
Inagaki, Y., Nakanishi, A. & Tange, T. A simple method for leaf and branch biomass estimation in Japanese cedar plantations. Trees 34, 349–356 (2020).
Bonan, G. B., Levis, S., Kergoat, L. & Olson, K. W. Landscapes as patches of plant functional types: An integrating concept for climate and ecosystem models. Glob. Biogeochem. Cy. https://doi.org/10.1029/2000GB001360 (2002).
Bonan, G. Ecological Climatology (Cambridge University Press, 2008).
R Core Team. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. https://www.R-project.org/ (2022).
Pallardy, S. G. Physiology of woody plants 3rd edn. (Elsevier, Berlin, 2008).
Tyree, M. T. & Zimmermann, M. H. Xylem Structure and the Ascent of Sap (Springer, 2002).
Niinemets, Ü. Is there a species spectrum within the world-wide leaf economics spectrum? Major variations in leaf functional traits in the Mediterranean sclerophyll Quercus ilex. New Phytol. 205, 79–96 (2014).
Lintunen, A. & Kalliokoski, T. The effect of tree architecture on conduit diameter and frequency from small distal roots to branch tips in Betula pendula, Picea abies and Pinus sylvestris. Tree Physiol. 30, 1433–1447 (2010).
Uggla, C., Mellerowicz, E. J. & Sundberg, B. Indole-3-acetic acid controls cambial growth in Scots pine by positional signaling. Plant Physiol. 117, 113–121 (1998).
Uggla, C., Moritz, T., Sandberg, G. & Sundberg, B. Auxin as a positional signal in pattern formation in plants. Proc. Natl. Acad. Sci. 93, 9282–9286 (1996).
Uggla, C., Magel, E., Moritz, T. & Sundberg, B. Function and dynamics of auxin and carbohydrates during earlywood/latewood transition in Scots pine. Plant Physiol. 125, 2029–2039 (2001).
Funada, R., Kubo, T. & Fushitani, M. Early- and latewood formation in Pinus densiflora trees with different amounts of crown. IAWA Bull. 11, 281–288 (1990).
Funada, R., Kubo, T., Tabuchi, M., Sugiyama, T. & Fushitani, M. Seasonal variations in endogenous indole-3-acetic acid and abscisic acid in the cambial region of Pinus densiflora Sieb. et Zucc. stems in relation to earlywood-latewood transition and cessation of tracheid production. Holzforschung 55, 128–134 (2001).
Sone, K., Suzuki, A. A., Miyazawa, S., Noguchi, K. & Terashima, I. Maintenance mechanisms of the pipe model relationship and Leonardo da Vinci’s rule in the branching architecture of Acer rufinerve trees. J. Plant Res. 122, 41–52 (2009).
Gray, H. R. The form and taper of forest-tree stems. Imperial Forestry Institute Paper no. 32. (Oxford University Press, 1956).
Van Pelt, R. Forest Giants of the Pacific Coast (University of Washington Press, 2001).
Sillett, S. C., Van Pelt, R., Carroll, A. L., Campbell-Spickler, J. & Antoine, M. E. Structure and dynamics of forests dominated by Sequoiadendron giganteum. For. Ecol. Manag. 448, 218–239 (2019).
Ishii, H., Ford, E. D. & Dinnie, C. E. The role of epicormic shoot production in maintaining foliage in old Pseudotsuga menziesii (Douglas-fir) trees II. Basal reiteration from older branch axes. Can. J. Bot. 80, 916–926 (2002).
Tanabe, T., Epron, D. & Dannoura, M. A new approach to identify the climatic drivers of leaf production reconstructed from the past yearly variation in annual shoot lengths in an evergreen conifer (Picea mariana). Trees 36, 179–190 (2022).
Falster, D. S. et al. BAAD: A biomass and allometry database for woody plants. Ecology 96, 1445 (2015).
Komsta, L. outliers: Tests for Outliers. R package version 0.15. https://CRAN.R-project.org/package=outliers (2022).
Warton, D. I., Duursma, R. A., Falster, D. S. & Taskinen, S. SMATR 3—An R package for estimation and inference about allometric lines. Methods Ecol. Evol. 3, 257–259 (2012).
Bates, D. et al. Fitting linear mixed-effects models using lme4. J. Stat. Softw. 67, 1–48 (2015).
Sprugel, D. G. Correcting for bias in log-transformed allometric equations. Ecology 64, 209–210 (1983).
Acknowledgements
This work was supported in part by the Japan Society for the Promotion of Science (JSPS) KAKENHI (Nos 24580209).
Author information
Authors and Affiliations
Contributions
A.S. and Y.I. conceived the analysis. A.S. wrote the manuscript. All authors contributed to the field data and reviewed the manuscript.
Corresponding authors
Ethics declarations
Competing interests
The authors declare no competing interests.
Additional information
Publisher's note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary Information
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Sumida, A., Inagaki, Y., Kajimoto, T. et al. Allometry of the quasi-pipe (qPipe) model for estimating tree leaf area and tree leaf mass applied to plant functional types. Sci Rep 13, 9954 (2023). https://doi.org/10.1038/s41598-023-37112-1
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/s41598-023-37112-1
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.