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References
Niu, M. et al. Droplet-based transcriptome profiling of individual synapses. Nat. Biotechnol. https://doi.org/10.1038/s41587-022-01635-1 (2023).
Duve, C. Exploring cells with a centrifuge. Science 189, 186–194 (1975).
Gray, E. G. & Whittaker, V. P. The isolation of nerve endings from brain. J. Anat. 96, 79–88.8 (1962).
Hafner, A.-S., Donlin-Asp, P. G., Leitch, B., Herzog, E. & Schuman, E. M. Local protein synthesis is a ubiquitous feature of neuronal pre- and postsynaptic compartments. Science 364, eaau3644 (2019).
Zhu, F. et al. Architecture of the mouse brain synaptome. Neuron 99, 781–799 (2018).
Dodd, P. R. et al. Optimization of freezing, storage, and thawing conditions for the preparation of metabolically active synaptosomes from frozen rat and human brain. Neurochem. Pathol. 4, 177–198 (1986).
Dunkley, P. R. & Robinson, P. J. Synaptosome preparations: which procedure should I use? In Synaptosomes 27–53 (ed. Murphy, K. M.) (Springer, 2018).
Villasana, L. E., Klann, E. & Tejada-Simon, M. V. Rapid isolation of synaptoneurosomes and postsynaptic densities from adult mouse hippocampus. J. Neurosci. Methods 158, 30–36 (2006).
Biesemann, C. Development of Fluorescence Activated Synaptosome Sorting (FASS) and Analysis of VGLUT1 Synapses from Mouse Brain. PhD thesis, Georg August University Göttingen (2010).
Biesemann, C. et al. Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting. EMBO J. 33, 157–170 (2014).
Hobson, B. D. & Sims, P. A. Critical analysis of particle detection artifacts in synaptosome flow cytometry. eNeuro 6, ENEURO.0009-19.2019 (2019).
Hobson, B. D. et al. Subcellular and regional localization of mRNA translation in midbrain dopamine neurons. Cell Rep. 38, 110208 (2022).
Raiteri, M., Sala, R., Fassio, A., Rossetto, O. & Bonanno, G. Entrapping of impermeant probes of different size into nonpermeabilized synaptosomes as a method to study presynaptic mechanisms. J. Neurochem. 74, 423–431 (2000).
Li, X. G., Somogyi, P., Ylinen, A. & Buzsáki, G. The hippocampal CA3 network: an in vivo intracellular labeling study. J. Comp. Neurol. 339, 181–208 (1994).
Shapiro, E., Biezuner, T. & Linnarsson, S. Single-cell sequencing-based technologies will revolutionize whole-organism science. Nat. Rev. Genet. 14, 618–630 (2013).
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B.D.H. and E.H. co-wrote and edited the manuscript. B.D.H. generated the figure with input from E.H.
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Hobson, B.D., Herzog, E. Methodological concerns and lack of evidence for single-synapse RNA-seq. Nat Biotechnol 41, 1221–1224 (2023). https://doi.org/10.1038/s41587-023-01877-7
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DOI: https://doi.org/10.1038/s41587-023-01877-7
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