Abstract
Homochiral α-amino acids are widely used in pharmaceutical design as key subunits in chiral catalyst synthesis or as building blocks in synthetic biology. Many synthetic methods have been developed to access rare or unnatural variants by controlling the installation of the α-stereocentre. By contrast, and despite their importance, α-amino acids possessing β-stereocentres are much harder to synthesize. Here we demonstrate an iridium-catalysed protocol that allows the direct upconversion of simple alkenes and glycine derivatives to give β-substituted α-amino acids with exceptional levels of regio- and stereocontrol. Our method exploits the native directing ability of a glycine-derived N–H unit to facilitate Ir-catalysed enolization of the adjacent carbonyl. The resulting stereodefined enolate cross-couples with a styrene or α-olefin to install two contiguous stereocentres. The process offers very high levels of regio- and stereocontrol and occurs with complete atom economy. In broader terms, our reaction design offers a unique directing-group-controlled strategy for the direct stereocontrolled α-alkylation of carbonyl compounds, and provides a powerful approach for the synthesis of challenging contiguous stereocentres.
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Main
Amino acids are arguably the most valuable homochiral building blocks available to synthetic chemists. This has stimulated the development of a variety of methods to access rare or unnatural variants, focusing predominantly on control of the α-stereocentre1. Exemplar catalytic asymmetric methods include Strecker reactions2, phase-transfer-catalysed alkylations of glycine imines3, alkene hydrogenations4, cross-couplings5 and conjugate additions6,7. Although highly effective, these approaches are not generally suitable for accessing amino acids possessing β-stereocentres (Fig. 1a). Substitution at this position has important ramifications for the three-dimensional structure of a derived peptide8,9, for example, or the physiochemical properties of a downstream product. As testament to this, a variety of biosynthetic processes are known that allow the β-functionalization of canonical amino acids10. A handful of catalytic asymmetric methods have emerged that allow the synthesis of certain β-stereogenic α-amino acids. These include biocatalytic dynamic kinetic resolutions11, diastereoselective C–H arylations12, asymmetric hydrogenations13 and stereoretentive cross-couplings14. These important approaches each have their own limitations and are non-trivial, requiring, for example, a preassembled framework and/or pre-installed homochirality and/or pre-functionalized reaction partners.
We questioned whether an alternative and more convergent approach could be achieved by the direct and stereocontrolled C–H alkylation of a glycine-based precursor (Fig. 1b). In particular, we targeted a process where the new C–C bond and the two stereocentres are established in a single operation. In essence, this requires the invention of a catalytic method that allows the enantio- and diastereoselective direct (that is, stoichiometric base-free) intermolecular α-alkylation of carbonyl compounds. For systems that lack additional strong acidifying groups3, this area has proven to be exceptionally challenging15,16, such that auxiliary-based approaches are still dominant in target-directed synthesis (Fig. 1c)17,18. Asymmetric ketone α-alkylation can be achieved from lithium enolates using catalytic quantities of a chiral amine ligand (Fig. 1d)19. Other catalytic enantioselective methods have emerged, but these are not usually direct, relying either on the pre-formation of an enolate or enolate equivalent20,21,22 or the pre-installation of sacrificial functionality23. In a key advance, a tricatalytic system was developed that promotes the direct linear selective α-alkylation of aldehydes (Fig. 1e)24. This process is also important because it harnesses readily available non-activated alkenes as alkylating agents for enantioselective α-functionalization reactions25. Also developed is an alternative Ir-catalysed C–H activation-based branch-selective process that offers promising levels of stereocontrol26. Although elegant, these methods are not applicable to the issue at hand, because they are reliant on a condensation event to generate an enamine.
In this Article we outline an alternative approach that is predicated on using the glycine-based N–H unit as a directing group (I) to trigger metal-catalysed ‘soft’ enolization en route to geometrically defined homochiral enolates of type II (Fig. 1b)27,28,29. At the outset, this proposition was considered tentative because of the low acidity of I. Nevertheless, based on our earlier studies involving N-directed C–C bond activation30, we were drawn to diphosphine-modified cationic Ir(I) systems as mild Lewis acids for the proposed enolization process (I to II). A synergistic benefit of these systems resides in the observation that they can also promote the non-enantioselective (and mechanistically unclear) branch-selective addition of highly activated 1,3-dicarbonyls across alkenes31,32,33. Accordingly, our reaction design required the metal catalyst to activate both a relatively non-acidic pronucleophile and a non-polarized proelectrophile. As outlined in the following, the realization of this approach (1) addresses the immediate issue of accessing β-substituted α-amino acids, (2) offers a unique directing-group-based approach to the direct stereocontrolled α-alkylation of low-acidity carbonyl compounds (Fig. 1f) and (3) provides a broader cross-coupling framework for the by-product-free and stereocontrolled installation of contiguous stereocentres, which is a formidable issue34. Very recently, we reported enantioselective decarboxylative Takeuchi-type processes that use highly acidic and directing 2-aza-aryl acetates as the pronucleophile35. Compared to this, the work described herein represents a major advance because it allows the α-alkylation of much less acidic C–H bonds, and the installation of contiguous stereocentres. Additionally, the directing mode has potentially wider generality and the processes are mechanistically distinct.
Results and discussion
As part of our early studies towards the envisaged process, we explored the potential C–H addition of amide-based systems 1a–f across styrene 2a (600 mol%). Because the nature of the N-substituent was deemed to be a critical factor, a range of options were evaluated using Ir(cod)2BARF (5 mol%) (cod = 1,5-cyclooctadiene; BARF = tetrakis(3,5-bis(trifluoromethyl)phenyl)borate) and (R)-BINAP (L1) (5 mol%) in toluene at 130 °C (Table 1). Carbamate (1a), sulfonamide (1b), amide (1c), N-benzhydryl (1d) and free amine (1e) systems were all ineffective. In contrast, aniline derivative 1f did lead to α-alkylation product 3fa in 86% yield, >30:1 branched:linear selectivity, 9:1 d.r. and 96:4 e.r. (entry 1). To improve on this remarkable preliminary result, other chiral diphosphine ligands were assayed, and this revealed that replacing L1 with (R)-SEGPHOS (L5) was beneficial (entries 2–9). Further studies established that 1,4-dioxane is an effective solvent, offering marginal improvements to enantioselectivity (entries 10–14). The precise nature of the precatalyst is important: counterions that are more strongly coordinating than BARF are less effective (entries 15–18), whereas use of an analogous Rh-complex was not successful (entry 19). Using the combination of Ir(cod)2BARF and L5, we optimized the loading of styrene, leading to the conditions outlined in entry 21, which use just two equivalents. The reaction temperature can be lowered to 110 °C (entry 23), although subsequent scope studies were conducted at 130 °C. In all entries, branched-to-linear selectivities exceeded 30:1.
We have explored the scope of the process, and found that it is effective for the coupling of a range of secondary and tertiary amide-based systems (1f–l) with styrene 2a (Table 2). Primary amides also participate with high enantioselectivity, but in more modest yield (Supplementary Fig. 1). Notably, ketone-based systems also participate; for example, using (R)-3,5-(t-Bu)2-8H-BINAP as the ligand (not depicted), phenyl ketone-based adduct 3ma was accessed in good yield, high diastereoselectivity and with promising enantioselectvity. Using L7 as the ligand, methyl-ketone-based system 3na was generated in 90:10 e.r.; here, C–C bond formation occurred at the more hindered side of the ketone, demonstrating a further benefit of the directing-group-controlled approach. The method even tolerates very hindered ketones, such that t-butyl system 3oa could be accessed in high yield. The scope of the directing N-aryl unit has been investigated, and this revealed that a broad range of systems are viable. Of particular note is the success of N-4-hydroxylphenyl (3pa and 3wa) and N-4-methoxyphenyl systems (3qa), as the aryl units of these products can be removed easily (vide infra). The method also offers very wide scope with respect to the R2 unit of the styrene coupling partner. A variety of electron-rich and electron-poor systems participated smoothly, including those possessing sterically demanding ortho-substitution (for example, 3fl). Heteroaryl- and ferrocenyl-substituted systems 2f and 2g cross-coupled with high degrees of efficiency. Notably, α-olefins can also participate smoothly, although these less reactive proelectrophiles require more readily enolizable ketone-based systems. L6 offered optimal efficiencies for t-butyl ketone 1o, delivering targets 3op–3os in 71:29 to 96:4 e.r. and 2:1 to 16:1 d.r. The tolerance of the protocol to a wide range of alkene coupling partners bodes well for further development and applications. To highlight the potential of the method for complex molecule synthesis, we prepared indomethacin-derived styrene 2t. Exposure of this to 1f under optimized conditions delivered target 3ft in 63% yield, 98:2 e.r., >30:1 branched-to-linear selectivity and 10:1 d.r. Although the protocol tolerates very sensitive functionality (for example, the –BPin unit of 3fh), certain limitations (for example, alkenes attached to basic heteroarenes, 1,1- and 1,2-disubstituted alkenes, ortho-substituted –NHAr units) have been identified, and these are summarized in Supplementary Fig. 2.
The initial alkylation products are readily derivatized to a range of potentially valuable amino-containing building blocks. For example, reductive manipulations of morpholino-amide 3ka provided selective access to the corresponding amino alcohol 4, aldehyde 5 and amine 6 (Fig. 2a). Weinreb-amide-like ketone syntheses are also possible, as demonstrated by the formation of 7 (ref. 36). Hydrolytic decarboxylation provided chiral amine 8 in 97:3 e.r.; decarboxylations of this type are unusual, and further studies are being undertaken to rationalize the facility of this process37. Systems possessing 4-hydroxyphenyl units on nitrogen can easily be deprotected to the free amine under oxidative conditions. For example, treatment of 3pa with [bis(trifluoroacetoxy)iodo]benzene provided amino-amide 9 in 96% yield (Fig. 2b). Derivatization of this to its corresponding p-bromophenyl amide 10 allowed determination of the relative and absolute configuration by single-crystal X-ray diffraction, and the stereochemistry of other catalysis products was assigned on this basis. Hydrolysis of the amide of 9 provided the corresponding amino acid 11, which is a critical subunit in a range of biologically relevant targets, including peptide β-turn mimics38, endomorphin analogues39 and the natural product bottromycin A2 (depicted)40. Compared to previous syntheses, this method for accessing 11 is notable for both its brevity and the level of stereocontrol. N-deprotection of 3la provided 12, which maps onto the subunit of mitogen-activated protein kinase kinase inhibitor candidates41. Similarly, we were able to access the subunit of a growth hormone promoter candidate by devising a two-step conversion of 1p to 13 (ref. 42). The ability to access chiral amines by decarboxylation of the initial catalysis products was exploited in the conversion of 1w to 14 via 3wk, offering access to the key subunit of the weight-loss drug (S)-lorcaserin (Fig. 2c)43.
Control experiments confirmed that C–C bond formation requires the Ir complex, the carbonyl unit and an NHAr unit (Supplementary Fig. 3). Exposure of 1f and 2a to optimized conditions but in the presence of D2O resulted in deuterium incorporation at C2 of both the product deuterio-3fa and the starting material deuterio-1f at partial conversion (Fig. 3a). Incorporation was also observed in the N-methyl groups, probably via reversible amide-directed C–H activation of this position (Supplementary Fig. 4)44. In the absence of the Ir complex, no deuterium incorporation was observed at C2 (Fig. 3b). Resubjection of diastereomerically pure product 3fa to the reaction conditions in the presence of D2O resulted in no epimerization and no deuterium incorporation at C2 (Fig. 3c), although incorporation was observed at the methyl groups (deuterio-3fa′), presumably as a result of reversible amide-directed C–H activation. Collectively, these results indicate that the envisaged Ir-catalysed enolization of 1f is feasible, whereas the product is resistant to this process, presumably because of the A(1,3)-strain that would arise in the resulting enolate. Efforts to isolate and characterize a chelate related to I or II have so far been unsuccessful, with NMR experiments indicating that this is not a resting state for catalysis. Using alkene 2j as the limiting reagent, natural-abundance 13C kinetic isotope effects (KIEs) were determined according to the Singleton method (Fig. 3d)45,46. Appreciable KIEs were observed at C1 (1.013) and C2 (1.008). Based on this, we currently favour a mechanism involving turnover-limiting carbometallation from an Ir–π complex. Accordingly, our working mechanistic framework is outlined in Fig. 3e. To initiate the process, N–H metallation of 1 provides I. Related metallations have been demonstrated within the context of C–C-bond activation processes using cationic Rh(I) complexes30. In the current scenario, the N-metallated unit of I functions as a Lewis acid to trigger enolization and provide II. At this stage, the Ir centre may or may not be deprotonated, with the former option depicted in Fig. 3e. Deprotonation could be facilitated by the –NHAr unit of another molecule of 1; note that exogenous bases (for example, Et3N, K2CO3) are detrimental to reaction efficiency. Our observations suggest the carbonyl unit must either be strongly coordinating (for example, an amide) to enhance access to I, or relatively acidifying (for example, a ketone) to facilitate enolization (cf. I to II). Thus, amide or ketone-based systems are effective, whereas, at the current level of development, ester analogues are not suitable. To facilitate carbometallation, the alkene component is then activated by π-coordination to either II or another Ir centre (III). To distinguish these options, graphical kinetic analysis was undertaken, revealing the order in catalyst to be ~2 (Supplementary Fig. 5)47. Accordingly, we favour a bimetallic pathway leading to IV, which then undergoes protodemetallation to release the product. Although not depicted, one or other of the Ir centres of IV may be coordinated to the carbonyl unit. The primary factor that governs enantioselectivity is probably the chiral information embodied within Ir-enolate II, because this is proximal to both reaction partners in the C–C-bond-forming step. The structural features of substrate 1 and π-coordinated alkene complex III are both likely to have a substantial influence on the diastereoselectivity of the process. The high branch selectivity during the conversion of II to IV may reflect electronic effects and/or a preference for the Ir centre of III to move to the less hindered end of the alkene.
Outlook and conclusions
The mechanistic analysis outlined in Fig. 3e raises many interesting possibilities for future development. For example, an intriguing option is to investigate whether the pronucleophile and the proelectrophile can be activated using two different metal complexes. This would raise interesting possibilities for effecting stereocontrol. Perhaps more simply, the current catalyst system might be suitable for a wider range of directing modes. To probe this, we investigated the α-alkylation of amide 15, where the directing group is moved one methylene unit further from the carbonyl unit (Fig. 3f). Remarkably, using styrene as the coupling partner, we were able to generate target 16 in 55% yield and with promising levels of selectivity48. Clearly, further refinement is required, but the result is important because it shows that the directed enolization approach has wider applicability. More broadly, our study is important because enantioselective C(sp3)–H additions to alkenes are achieved by exploiting ‘native’ directing functionality. This has a clear parallel to Murai’s seminal ortho-directed alkene hydroarylations49, a report that ignited the field of metal-catalysed C(sp2)–H functionalization, and has led to an emerging family of by-product-free enantioselective cross-couplings50. Our laboratory is now focused on developing a complementary set of C(sp3)–H-based cross-couplings by harnessing the design principles outlined here.
Data availability
The Supplementary Information contains experimental procedures and data that support this study, including Supplementary Figs. 1–5 and analytical data listings and NMR spectra. Crystallographic data for the structures reported in this Article have been deposited at the Cambridge Crystallographic Data Centre under deposition nos. CCDC 2245009 (10) and 2246104 (3fa). Copies of the data can be obtained free of charge via https://www.ccdc.cam.ac.uk/structures/.
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Acknowledgements
We thank the Chinese International Postdoctoral Exchange Fellowship Program (fellowship to F.H.), the Bristol Chemical Synthesis Centre for Doctoral Training funded by the EPSRC (EP/M507994/1) and AstraZeneca (studentship to T.P.A.), the ERC (grant no. 863799 ‘ChiCC’ to J.F.B.), and the University of Liverpool (Regius Chair to J.F.B.) for financial support. We thank K. Luzyanin (Liverpool) for advice on NMR analysis, and the University of Liverpool X-ray crystallography service for analysis of 3fa and 10.
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F.H. evaluated the reaction conditions and conducted mechanistic studies. F.H. and T.P.A. evaluated the reaction scope. All authors analysed data. P.D.K. and J.F.B. designed and directed the work. J.F.B. wrote the manuscript with contributions from all the authors.
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F.H., T.P.A. and J.F.B. declare the following competing interests: A patent has been filed by the University of Liverpool on the chemistry described in this manuscript (applicant, The University of Liverpool; title, ‘Alkylation process’; UK patent application no. 2306313.4). The patent covers the alkylation process described in this manuscript. P.D.K. declares no competing interests.
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Supplementary Information
Supplementary Figs. 1–5, experimental procedures, analytical data, spectra.
Supplementary Data 1
Crystallographic data for compound 3fa; CCDC reference 2246104.
Supplementary Data 2
Crystallographic data for compound 10; CCDC reference 2245009.
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Hong, F., Aldhous, T.P., Kemmitt, P.D. et al. A directed enolization strategy enables by-product-free construction of contiguous stereocentres en route to complex amino acids. Nat. Chem. (2024). https://doi.org/10.1038/s41557-024-01473-5
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DOI: https://doi.org/10.1038/s41557-024-01473-5