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A high-throughput workflow for comprehensive glycopeptide mapping

Nature Biomedical Engineering volume 8, pages 212–213 (2024)Cite this article

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We developed OxoScan-MS, a mass spectrometric acquisition technology for high-throughput quantification of glycopeptides. When applied to plasma of patients with COVID-19, OxoScan-MS revealed differential glycosylation in disease-relevant proteins. This approach offers potential for large-scale applications, moving beyond traditional protein abundance measurements to explore glycosylated protein biomarkers.

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Fig. 1: Oxonium ion maps generate a specific and quantitative glycoproteome analysis of neat human plasma.

References

  1. Anderson, N. L. & Anderson, N. G. The human plasma proteome: history, character, and diagnostic prospects. Mol. Cell. Proteomics 1, 845–867 (2002). A review article that discusses the human plasma proteome.

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This is a summary of: White, M. E. H. et al. Oxonium ion scanning mass spectrometry for large-scale plasma glycoproteomics. Nat. Biomed. Eng. https://doi.org/10.1038/s41551-023-01067-5 (2023).

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A high-throughput workflow for comprehensive glycopeptide mapping. Nat. Biomed. Eng 8, 212–213 (2024). https://doi.org/10.1038/s41551-023-01068-4

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