Abstract
Metastases originate from primary tumors and reach distant organs. Growing evidence suggests that metastases are under the control of primary tumors even outside the primary site; however, the mechanisms by which primary tumors remotely control metastases remain unclear. Here, we discovered a molecular mechanism by which primary tumors suppress metastatic growth. Interestingly, we found that extracellular vesicles (EVs) derived from the primary tumor can inhibit the growth of metastases both in vitro and in vivo. miR-1 was particularly enriched in primary tumor-derived EVs (pTDEs) and was found to be responsible for the suppression of metastatic growth. Mechanistically, intracellular reactive oxygen species (ROS) production and DNA damage were induced, which led to cell cycle arrest. Collectively, our data demonstrate that primary tumors restrict the growth of distant metastases via miR-1 in pTDEs and that miR-1 could potentially be used as an antimetastatic agent.
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Introduction
Metastases that have migrated from primary tumors have three potential fates after settling in a secondary organ. First, they may undergo metastatic outgrowth; second, they may die via mechanisms involving immune cells or apoptosis; and finally, they may take on a dormant state; metastasis accounts for approximately 70% of cancer-related deaths1,2. Metastases that undergo outgrowth can be effectively targeted with antiproliferative drugs. However, targeting dormant cancer cells is a challenge, as clear treatment targets have not been defined3. Even after metastatic cells have migrated from the primary tumor and settled elsewhere, they remain under the control of the parental primary tumor. Primary tumors play dual roles: they promote the dissemination of metastatic cells from the primary site while also maintaining control of their behaviors4,5,6. Many studies have revealed how primary tumors influence the tumor microenvironment, thereby restraining metastatic growth through various dormant mechanisms, such as interfering with angiogenesis (angiogenic dormancy) or eliminating by immune cells (immunologic dormancy)7,8,9. One fundamental finding is that angiostatin and endostatin interrupt metastatic angiogenesis and lead to nutrient starvation10,11. Furthermore, primary tumors indirectly restrict metastasis by remodeling CD8+ cytotoxic T cells and macrophages to eliminate them4,6,12. Consequently, one pivotal factor contributing to the sudden increase in metastases after primary tumor treatment may be the lack of metastatic growth inhibition signals from the primary tumor.
Since the discovery of extracellular vesicles (EVs), cells have been known to deliver their signals to distance cells by wrapping such signals in a lipid bilayer13. EVs contain proteins, lipids, and nucleic acids. Research on the biological function of EVs in cancer has focused mainly on their role in tumorigenesis and the formation of the tumor microenvironment14, and there have been a few attempts to address the function of EVs as controllers of metastases15,16,17,18. Despite numerous studies on tumor-derived EVs (TDEs), there is no evidence showing the role of the primary TDEs (pTDEs) during metastasis.
In this study, we demonstrated that primary tumors use EVs to control metastatic growth. When small RNA sequencing was performed, miR-1 was found to be more abundant in pTDEs than in metastatic tumor-derived EVs (mTDEs). Additionally, we observed a reduction in the expression of miR-1 target genes in metastatic cancer cells treated with pTDEs. Moreover, we found that exosomal miR-1 acts as a key regulator of tumor inhibition and that engineered pTDEs with high levels of miR-1 markedly inhibited metastasis in both cell and mouse models. These findings highlight the role of miR-1 in pTDEs in metastastic growth inhibition.
Materials and methods
Cell culture
Six canine mammary gland adenocarcinoma cell lines (CHMp, CHMm, CIPp, CIPm, CTBp, and CTBm) were purchased from the N. Sasaki laboratory19 and grown in RPMI 1640 medium (HyClone, SH30027) supplemented with 10% fetal bovine serum (FBS; Gibco 1600044) and 50 μg/ml gentamicin (Sigma‒Aldrich, G1272). CHMp and CHMm cells were transfected with a firefly luciferase gene-expressing plasmid (Addgene #18964) using Lipofectamine 3000 reagent (Invitrogen, LM3000015). Culture medium containing geneticin (G418 sulfate) was used to select stably transfected cells (Gibco, 10131035). Twenty-four hours after transfection, the cells were supplemented with G418 (500 µg/ml) and maintained for one week to eliminate untransfected cells. Single cells were isolated from stable luciferase-expressing cells to establish a stable cell line and maintained with 250 µg/ml G418 for two weeks. Luciferase expression was confirmed with a luciferase assay. Human umbilical vein endothelial cells (HUVECs) were grown in endothelial cell basal medium-2 (EGM-2, Lonza, CC-3156) supplemented with EGM™-2 singleQuots® (Lonza, CC-4176). All cells were grown in a humidified incubator at 37 °C with 5% CO2 and confirmed to be negative for mycoplasma contamination.
Mouse experiments
All mouse experiments were conducted in accordance with the Seoul National University Institutional Animal Care and Use Committee (IACUC) guidelines, and the animal protocol was approved (SNU-210323-1-2). Nude mice (CrTac:NCr-Foxn1nu) were kept in pathogen-free conditions and maintained under a 12 h light/dark cycle at a controlled room temperature (22 ± 2 °C). A metastasis model was generated in 5-week-old female nude mice following orthotopic injection of a total of 5 × 105 luciferase-labeled CHMp and CHMm cells into the mammary fat pad, and resection surgery was performed 21 days postimplantation. Mice were anesthetized, and primary tumors were resected. Mice that underwent surgery were monitored for symptoms of pain and were sacrificed via inhalation of carbon dioxide (CO2). IVIS bioluminescence imaging was carried out during the formation of spontaneous lung metastases. All mice were randomized before injection of EVs and blindly selected before injection. For EV injection, all the treatments were administered via intravenous injection in a final volume of 150 µl. Mice were treated with EVs six times at two-day intervals. The experimental endpoint was established according to IACUC guidelines, and the maximal tumor volume was never exceeded.
Extracellular vesicle isolation and labeling
EVs were purified from cells cultured under serum-free conditions using a combination of ultrafiltration and ultracentrifugation. The graphical method is illustrated in Supplementary Fig. 2a. The cells were grown to 80% confluence, washed two times with PBS and incubated in serum-free medium for 24 h. First, the cell culture supernatant was subjected to differential centrifugation to eliminate cells, dead cells, and cell debris and filtered sequentially with 0.45 and 0.22 μm filters. The filtered supernatant was concentrated using 10 K Amicon Ultra 15 Centrifugal Filter Units (Merck, UFC903024). The filtered units were sequentially centrifuged. The mixture was ultracentrifuged for 80 min and washed with PBS. An EV concentration of 0.1 µg/ml was used in all in vitro assays.
EVs were isolated from biological fluids using the following commercial kit: ExoQuick exosome precipitation solution (System Biosciences, SBI-EXOQ5A-1). The plasma and serum samples were centrifuged at 3000 × g for 15 min to remove cells and cell debris. ExoQuick was added to the supernatant at an appropriate volume and incubated for 30 min at 4 °C. Pelleted EVs were resuspended in Qiazol (Qiagen, 79306) for RNA isolation and in urea/SDS lysis buffer for protein isolation. The isolated EVs were stored at −80 °C for later use.
EVs were labeled with PKH67 lipophilic membrane dye (Sigma, MNI67-KIT) following the manufacturer’s instructions. In brief, isolated EVs were resuspended in 1 ml of Diluent C, after which 6 µl of PKH67 dye was added. The mixture was incubated for 5 min at room temperature and centrifuged at 100,000 × g for 80 min.
Nanoparticle tracking analysis (NTA)
NTA was used to characterize the size and concentration of EVs from the cell culture supernatant and biological fluids using the NanoSight LM10 model (Malvern). The samples were diluted with PBS (0.22 µm filtered) and injected into the laser chamber. The data were analyzed by NTA v3.2 software.
Transmission electron microscopy (TEM)
The morphology of the EVs was analyzed by TEM using a Talos L120C system (Czech). Briefly, the samples were stained with a negative staining method using 2% uranyl acetate. One drop of diluted EVs was dropped on a glow-discharged copper/carbon-coated grid. After 1 min, the grid was drained using filter paper, and one drop of 2% uranyl acetate was added. The staining solution was removed, and the samples were observed via TEM (120 kV).
Small RNA sequencing and data analysis
The miRNA-seq library was prepared using the Small RNA Library Prep Kit (Nextflex) and sequenced as 100 bp or 150 bp paired-end reads on the Illumina HiSeq 3,000 and NovaSeq 6,000 platforms. To remove adapters with low-quality reads and extract miRNA-specific sequences, cutadapt was used with the following options: quality-base 33-u 4-m 22-M 30-f fastq-q 20-O 6-j 23-a adapter sequence. In this step, two different adapter sequences (TGGAATTCTCGGGTGC-CAAGG and GATCGTCGGACTGTAGAACTCTGAAC) were used for forward and reverse paired-end sequencing. Because trimmed reads are short (22–30 bp), forward and reverse reads in the same sample were merged into one fastq-formatted file. Before and after the trimming step, the quality of the sequenced reads was estimated using FastQC.
For known and novel miRNA analysis, the miRDeep2 package was used. Before analysis, the following two necessary files were prepared: (1) sequence files of mature and hairpin forms of dog miRNAs, which were downloaded from the miRbase database and extracted using the extract_miRNAs.pl script; and (2) indexed files from the dog reference genome (CanFam3.1) using Bowtie. First, all filtered read data were merged into one file for novel miRNA analysis. The merged data were converted to a collapsed FASTA-formatted file and aligned to the reference genome using the mapper.pl script with the options (-e -h -j -m -p). Second, novel miRNAs were identified using the miRDeep2.pl script. The identified mature and hairpin forms of novel miRNAs were extracted and combined with known forms of miRBase prepared previously. Finally, the expression values of known and novel miRNAs were calculated using the quantifier.pl script. The counts per million (CPM) values, which are scaled by the total number of reads, were used for further analysis. MiRDeep2 analysis provides a miRNA score ranging from -10 to 10; a higher score represents a genuine miRNA. We set a cutoff of 4 for strict identification of novel miRNAs. To estimate the reproducibility of the data between replicate samples, Pearson correlation values were calculated and visualized using the correlation function in R. For differentially expressed miRNA analysis, fold changes in expression and significance (P value) were calculated using the EdgeR package in R. With these calculated values, a volcano plot was generated through the ggplot package in R.
miR-1 mimic transfection
Synthetic microRNA mimics were used to overexpress and overload microRNAs in cells and EVs. CHMm cells were transfected using the lipid carrier Lipofectamine RNAiMAX (Invitrogen, 13778150) following the manufacturer’s instructions. Fifty picomoles of miR-1 mimics were mixed with RNAiMAX reagent and then incubated for 15 min at RT. The miR-1 and RNAiMAX complexes were added to the cells, which were then incubated for 24 h at 37 °C in a CO2 incubator. EVs were transfected using 0.3 M CaCl2 following the modified CaCl2-mediated transfection method20. Forty micrograms of EVs was mixed with 100 pmole of miR-1 mimics in BPS supplemented with 0.3 M CaCl2 and incubated on ice for 30 min. Then, the mixture was heat-shocked at 42 °C for 60 s and incubated on ice for 5 min. Transfected EVs were isolated again by ultracentrifugation and washed with PBS.
Clinical specimens
All study protocols and specimen collection steps were approved by the Institutional Review Board (IRB). The IRB was approved by the Seoul National University (IRB#SNU 16-10-063). Blood sample collections from 9 healthy controls and 31 breast cancer patients performed in accordance with established guidelines. Informed consent for specimen collection was obtained from all subjects, including both humans and dog guardians, when they were enrolled.
Statistical analysis
The data represent the mean ± SEM. Statistical analysis was performed using Prism software (v.8.0.1, GraphPad Software). To assess the statistical significance of differences among multiple groups, 2-way ANOVA combined with Tukey’s honest significant difference (HSD) test was performed, and Student’s t test was performed for comparisons between CHMp and CHMm cells. Significant differences are indicated with different symbols (*P < 0.05, **P < 0.01, and ***P < 0.001) in each figure legend. The number of experimental repeats and the value of n are also indicated in the figure legends.
Results
EVs from primary tumors inhibit the growth of metastases
To investigate the role of pTDEs in controlling metastases, we used two types of metastatic models. The first model is the spontaneous recurrence model, in which metastases grow after surgical removal of the primary tumor. For the second model, cell lines of both primary and metastatic tumors established from the same spontaneous cancer patient were used. Since there is no pair of naturally occurring human breast cancer (HBC)-derived primary and metastatic tumor cell lines, we used cells derived from a canine mammary gland tumor (CMT), as this tumor type has recently been reported to have pathological and molecular aspects similar to those of HBC21,22. The pair of CMT cell lines (primary/metastatic, CHMp/CHMm) originated from the same patients. In contrast to previous studies on primary tumors and metastases, we used cell lines derived from the primary tumor and metastasis of a single subject to increase the accuracy of our analysis.
First, for the metastatic mouse model, we used surgical methods described by Piranlioglu et al.6. Primary tumors were surgically excised 21 days after CHMp cell injection (Supplementary Fig. 1a–c). After surgery, mice that underwent sham surgery exhibited cancer growth concentrated at the primary site rather than developing distant metastases (Supplementary Fig. 1d). In contrast, the majority of the mice were confirmed to be free of residual primary tumors and were injected with pTDEs or the control. pTDEs were isolated as described in the Materials and Methods section, and the purified EVs were cup-shaped and positive for TSG101 and Alix (Supplementary Fig. 2a–d). PKH67-labeled pTDEs were found to be highly enriched in disseminated tumors and primary tumors (Supplementary Fig. 3a). The injection schedule is depicted in Fig. 1a. Subsequently, the mice were divided into two groups: one group was regularly injected with pTDEs, and the other group was injected with PBS as a control. Bioluminescence imaging revealed little metastatic growth in the pTDE group compared to the control group on the 39th day (Fig. 1b, c; Supplementary Fig. 3b). Metastases occurred in 3 of the 4 control mice, but very minor signals were observed in 2 of the 5 mice injected with pTDEs (Fig. 1b, c). There were more metastatic nodules in the lungs of the control mice than in those of the pTDE-injected mice (Fig. 1d, e). In a mouse model, the removal of primary tumors was shown to promote the growth of metastatic cancers in the clinic23,24,25,26. By comparison with the negative control, we demonstrated that when pTDEs were injected into spontaneous metastases generated after the removal of the primary tumor, they exerted an inhibitory effect on tumor growth, even in metastases that were previously undetectable. Collectively, our data reveal the pivotal role of pTDEs in metastatic growth inhibition.
pTDEs autonomously suppress metastatic growth in direct and indirect manners
Since primary tumors affect metastatic cancer cells both directly and indirectly through immune cells and endothelial cells that constitute the tumor mass, we next examined the impact of treating cancer cells and endothelial cells with pTDEs. We used a second metastatic model in which CHMm (metastatic) cancer cells were treated with pTDEs or mTDEs as a control to rule out any influences resulting from changes in the process of EV purification. CHMm cells treated with pTDEs exhibited reduced viability and proliferation, whereas the viability and proliferation rates of CHMm cells treated with mTDEs were not significantly different from those of the PBS control group (Fig. 2a, b). Moreover, more pTDEs accumulated in the G2/M phase of the cell cycle in the pTDE treatment group than in the control group (Fig. 2c). Since the accumulation of cells in the G2/M phase can be caused by cellular stress, such as cell oxidation, DNA replication, and transcription27,28, we investigated whether pTDE treatment induces cell stress. Treatment with pTDEs caused a marked increase in intracellular ROS levels (Fig. 2d) and in the level of γ-H2A.X, a sensitive marker of damaged DNA (Fig. 2e, f). These results indicate that pTDEs induce an increase in intracellular reactive oxygen species (ROS) levels, genomic instability, and accumulation in the G2/M phase and suppress the growth of metastatic cells. However, this growth inhibition effect was not related to cell death or migration (Supplementary Fig. 4a–c).
In contrast, pTDEs had a strong effect on the tube formation of endothelial cells (Fig. 2g). Human umbilical vein endothelial cells (HUVECs) incubated with pTDEs showed a 1.5-fold decrease in the area of meshes compared to that in the cells incubated with PBS, which formed regular tubes. These findings revealed that pTDEs inhibit metastasis through direct inhibition of metastatic tumor cell growth in mice and indirectly influence endothelial cells in the tumor microenvironment through the inhibition of angiogenesis.
Primary tumor stem cell-derived EVs restrict metastatic growth
It is speculated that cells that disseminate from primary tumors and settle in distant locations are less likely to thrive in harsh environments if they lack stemness features29,30,31,32. Therefore, we examined the cancer stemness features of primary tumors to determine the inhibitory effect of these tumors on metastasis. Although many studies have investigated the effect of primary cancer stem cell-derived EVs on disseminated cancer cells (DTCs), no reports have clearly addressed the antitumor effects of these EVs. To further investigate how primary tumors control metastases, we compared the stemness features of primary and metastatic tumors (Supplementary Fig. 5a). The \({{CD}44}^{+}\)/\({{CD}24}^{-}\) expression pattern, which is representative of breast cancer stem cells (CSCs), was observed for a greater percentage of CSCs in the primary tumor than in the metastatic tissue (Fig. 3a). In addition, other breast CSC characteristics, such as rapid cell proliferation, high aldehyde dehydrogenase (ALDH) enzyme activity, and mammosphere formation, were substantially enhanced in CHMp cells vs. in CHMm cells (Fig. 3b–d). The spheroids were fluorescently labeled with CD44 and CD24, and only the CSC populations could form spheroids (Fig. 3e). We further compared the CD44, CD24, and ALDH1A1 protein levels and the levels of CSC-related genes between CHMp and CHMm cells (Supplementary Fig. 5b, c and Supplementary Table 1); the assessed genes included CD44, ALDH, drug resistance-related ABC transporters, stemness factors, and epithelial–mesenchymal markers. Interestingly, compared with CHMm cells, CHMp cells had higher CD44, ALDH, and ABCG2 expression levels and higher levels of mesenchymal markers. Moreover, the CSC-rich CHMp cells formed larger and faster-growing tumors than did the CHMm cells and showed vast differences even when the same number of cells were inoculated (Fig. 3f–h and Supplementary Fig. 5d). All these data suggest that primary tumor CHMp cells have more CSCs than CHMm cells.
We further investigated whether primary tumors with stemness features produced distinct EVs that could exert an antimetastatic growth effect. We further used other primary and metastatic cells, CIPp and CIPm cells (Supplementary Fig. 5a), which exhibited fewer differences in CSC features (Fig. 3i). Notably, there was no significant difference in the expression of genes associated with ALDH or drug resistance- or stemness-related transcription factors except Nanog between CIPp and CIPm cells (Supplementary Fig. 5e). Thus, we sorted the population of \({{CD}44}^{+}\)/\({{CD}24}^{-}\) cells, which we named CIPp-CSCs (Fig. 3j). EVs were isolated from the maintained CIPp-CSC portion and added to CIPm cells. CIPp-CSCs decreased cell viability (Fig. 3k) and inhibited proliferation (Fig. 3l). CIPp-CSC-derived EVs significantly suppressed metastatic cell proliferation compared with CIPp-derived EVs. These data confirmed that the growth-inhibitory effect of EVs derived from primary tumors is associated with the stemness of the CSCs that constitute the primary tumor. Therefore, we examined the cancer stemness features of primary tumors to determine the inhibitory effect of these tumors on metastasis. Analyses of cancer stemness features in the primary tumor cell lines CHMp and CIPp revealed that nearly 80% of CHMp cells present cancer stemness features (\({{CD}44}^{+}\)/\({{CD}24}^{-}\)/\({{ALDH}}^{{high}}\)); CIPp also has a greater proportion of cells presenting stemness features than its metastatic cell line, but the proportion is not as high. Moreover, EVs derived from the CD44+/CD24− population of CIPp cells had markedly greater antitumor activity than EVs derived from the whole CIPp cell population, suggesting that this CD44+/CD24− population is involved in the suppression of metastasis.
miR-1 is enriched in pTDEs and suppresses target gene expression in recipient metastases
To determine which factors play a role in the effects of pTDEs, we performed small RNA sequencing (sRNA-seq). The procedures for RNA acquisition and QC data from small RNA sequencing are described in Supplementary Fig. 6a–e.
We identified 307 and 249 miRNAs from pTDEs and mTDEs, respectively (Fig. 4a, b and Supplementary Table 2, 3). The term “miRNA involved in cell proliferation” was the top Gene Ontology (GO) term for the pTDE miRNAs (Supplementary Fig. 6f, top). The term ‘regulation of stem cells’ was the main enriched term for mTDE miRNAs (Supplementary Fig. 6f, bottom). Since one miRNA silences multiple genes, we investigated miRNA target genes using TargetScan and miRDB, which include reference genomes for dogs (Supplementary Table 4). Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that the target genes of the pTDE miRNAs were related to cellular proliferation pathways, such as the cAMP, cGMP-PKG, PI3K-Akt and TNF signaling pathways (Supplementary Fig. 6g). Based on the GO and KEGG analyses, exosomal miRNAs might be the main factor mediating the inhibition of proliferation of metastases induced by pTDEs. Next, we identified the miRNAs that were differentially expressed in pTDEs and mTDEs. Notably, among the miRNAs found in the pTDEs, cfa-miR-1-1 and -2 had the highest expression levels (Fig. 4c). In addition, from the miRNA–gene network analysis, miR-1 had the highest number of nodes among the miRNAs and the highest degree of miRNAs known to play a distinct role in tumor suppression (Fig. 4d).
Interestingly, the difference in the expression pattern of miR-1 between RNase-treated pTDEs and mTDEs corresponded with the difference between CHMp and CHMm cells (Fig. 4e). We integrated two databases (TargetScan and miRDB) that can retrieve miR-1 target genes and sorted 293 common genes (Fig. 4f). KEGG and Reactome analyses of the miR-1 target genes revealed a significant association with the cell cycle (Fig. 4g, h). Next, we selected ten predicted miR-1 target genes (HACE1, C5orf51, PTPLAD1, GLCCI1, MMD, GJA1, THSB4X, SCAF11, WNK3, and SMIM14) and assessed whether they were affected by pTDEs via qRT‒PCR. The levels of HACE1, C5orf51, PTPLAD1, GLCCI1, and MMD were significantly decreased by pTDE treatment in CHMm cells (Fig. 4i). In contrast, the other five genes did not significantly change (Supplementary Fig. 7a). Treatment with pTDEs also reduced the protein level of HACE1, which subsequently resulted in an increase in the level of the Rac1 protein, a target of the HACE1 E3 ligase33,34 (Fig. 4j). The regulation of the miR-1-HACE1-Rac1 axis could help explain how pTDEs cause growth inhibition in recipient metastatic cells. Taken together, these findings indicate that miR-1 in pTDEs can reduce HACE1 levels in recipient cells and lead to the accumulation of Rac1, which increases ROS levels and subsequently induces cell cycle arrest after DNA damage, slowing cell proliferation. Overall, pTDEs from primary tumors express miR-1, which might have a role in the suppression of metastasis.
pTDEs-miR-1 have an antimetastatic effect in an in vivo mouse model
To verify that the inhibition of metastatic growth by pTDEs is due to high levels of miR-1, we treated CHMm cells with miR-1 (Supplementary Fig. 8). Treatment with liposomes containing miR-1 dramatically decreased the expression of the six target genes of miR-1 (Supplementary Fig. 8a). Cell viability and growth were also significantly reduced in the group treated with miR-1 (Supplementary Fig. 8b, c). Additionally, similar to conventional pTDE treatment, miR-1 treatment increased the proportion of cells in the G2/M stage of the cell cycle (Supplementary Fig. 8d). Furthermore, similar to the effects of pTDEs, miR-1 increased intracellular ROS levels and genomic instability (Supplementary Fig. 8e, f). Finally, miR-1 increased the levels of γ-H2A and Rac1 and decreased the level of HACE1 (Supplementary Fig. 8g). Overall, similar to the effects of pTDEs, miR-1 induced genomic instability, promoted G2/M phase retention, and suppressed proliferation.
We then engineered pTDEs overloaded with miR-1 (pTDEs-miR-1); this process resulted in an approximately 500-fold increase in the miR-1 concentration compared to that in pTDEs (Fig. 5a). Compared with those of pTDEs, the properties of pTDEs-miR-1 were significantly enhanced; for example, target gene levels, cell viability, and cell growth were further decreased, and G2/M phase arrest, ROS accumulation, DNA damage, and dormancy were further promoted in the pTDE-miR-1-treated group compared to the pTDE-treated group (Fig. 5b–g and Supplementary Fig. 8h, i). These results demonstrated that primary tumor-mediated metastatic growth inhibition primary tumors by metastasis can be mediated by miR-1 present in pTDEs.
Next, we examined the ability of pTDEs-miR-1 to inhibit metastatic growth in an animal model. As shown in Fig. 1, after removal of the primary tumor from the CMT mouse model, mice confirmed to have metastases in a secondary organ (lung) were grouped and treated with PBS, pTDEs, or pTDEs-miR-1. The injection schedule is depicted in Fig. 5h. As indicated by the radiance data, metastatic growth was dramatically suppressed in the pTDE group but was mostly suppressed in the pTDEs-miR-1 group compared to that in the PBS group (Fig. 5i, j). Lungs from the PBS-, pTDE-, and pTDE-miR-1-injected mice showed significant differences in metastatic nodule counts (Fig. 5k). Metastases were easily found in mouse lung tissues from the PBS group but scarcely found in those from the pTDE group, and the metastatic tumors were smaller in the pTDE group and much smaller in the pTDEs-miR-1 group than in the PBS group (Fig. 5l). Overall, these data strongly suggest that EVs derived from primary tumors and loaded with miR-1 can inhibit the formation and growth of metastases that have already formed in lung tissues.
Blood exosomal miR-1 levels can be a diagnostic marker for metastases in clinical specimens
We then investigated whether exosomal miR-1 can be detected in the blood of individuals with CMT and HBC (Fig. 6a). Patient information is described in Supplementary Table 5. EVs were isolated from plasma and serum obtained from the patients and were found to be cup-shaped and less than 150 nm in size (Fig. 6b, c). We observed that the expression of exosomal miR-1 was greater in both CMT and HBC patients than in healthy controls; furthermore, the expression of exosomal miR-1 increased gradually with the progression of breast cancer (Fig. 6d, e). The largest amount of exosomal miR-1 was detected in the sera of Stage III patients, suggesting that primary tumors in locally advanced metastatic stages of breast cancer might generate an increased inhibitory signal from exosomal miR-1 to inhibit the growth of hidden metastases.
Next, we investigated the association between miR-1 expression and patient survival using Kaplan‒Meier plotter. We found that the high miR-1 expression group had better overall survival (OS) than the low miR-1 expression group for both lymph node-negative and lymph node-positive patients, as well as for all patients. High levels of miR-1 in tumor tissues may lead to high levels of exosomal miR-1 in the blood, which could result in further suppression of metastasis. However, the OS patterns depending on HACE1 expression differed between groups based on metastasis status, as high HACE1 expression was associated with worse OS only in the presence of metastasis (Fig. 6f). Overall, exosomal miR-1 is significantly increased in both human breast cancer and canine mammary tumors. Moreover, exosomal miR-1 levels increased with increasing tumor grade, indicating a potential association with the progression of metastasis.
Discussion
One of the key questions regarding the mechanisms of metastasis is how the primary tumor controls the tumor cells that have disseminated from the primary site. Primary tumor-derived cells migrate to blood vessels to become circulating tumor cells (CTCs), invade blood vessels and enter several other organs. CTCs travel alone or in clusters and maintain a dormant state to evade immunosurveillance mediated by immune cells in the blood35,36. If these CTCs find a conducive environment, they exit circulation and establish a new site of residence. These disseminated cells settle and grow in preferred organs, a phenomenon known as organotropism. After arriving at a secondary site, DTCs emerge from dormancy and retain their stem cell properties to reinitiate tumor growth37,38. The emergence of clinically significant metastatic tumors is crucial because previously quiescent DTCs reactivate and regain stem cell-like properties that facilitate self-renewal and the potential for continued tumor growth39. However, the underlying mechanisms governing the transition between dormant and awakened states in these cells, including the factors that determine their ultimate fate at secondary sites, have not been fully elucidated. Recently, Borriello et al. showed that metastasizing tumor cells are kept in a dormant state at secondary sites in the lung by primary tumor-associated macrophages4. Primary tumors were reported to inhibit the growth of metastases by inducing apoptosis in DTCs directly or by regulating immune cells and endothelial cells in the tumor microenvironment4,6,11,40,41,42. Because primary tumors inhibit DTCs, when the primary tumor disappears, DTCs start to grow, regardless of where they are located, due to release from growth inhibition43. This finding is supported by the fact that early-stage surgical removal (on Day 4 after initial tumor implantation) of primary breast tumors in mice induces micrometastases in the lymph nodes because DTCs are present in these nodes at this stage42. Conversely, late-stage surgery (on Day 13) results in the formation of distant organ metastases, such as those in the lungs, as DTCs have sufficient time to disseminate and colonize these organs44. Therefore, surgical removal of primary tumors remains a controversial topic in the field of cancer research. While this treatment can improve patient survival and drug accessibility45, it can also promote the growth of metastases46,47. Here, we revealed that DTCs cannot grow in a dormant state because of the presence of EVs derived from primary tumors. We also showed that the absence of EVs due to the absence of a primary tumor allows the growth of DTCs, while primary tumor-derived EVs can inhibit the growth of DTCs, even exhibiting a therapeutic effect.
To demonstrate how primary tumors can inhibit the growth of metastases, we studied the fate of DTCs after the removal of primary tumors in an ideal model. The growth inhibition induced by pTDEs clearly suggested that the primary tumor induces antitumor effects to prevent the development of metastasis (Fig. 1). We demonstrated that this antitumor effect of EVs was caused by the accumulation of ROS and damaged DNA. However, DNA damage results in an increase in the percentage of cells in the G2/M phase of the cell cycle, leading to growth arrest. Next, we found that EVs in the tumor mass can exert effects similar to endostatin, which has been reported to be an antiangiogenic factor by Folkman11. Furthermore, pTDEs limit the metastatic outgrowth of DTCs by disrupting endothelial cell formation of capillary-like structures. Consistent with our findings, the clearance of tumor cells in the EV-injected mouse model was mediated by both slow-cycling tumor cells and antiangiogenic agents (Fig. 2). Other reports have indicated that primary tumors can induce antitumor immunity by priming T cells and macrophages4,6. Due to the immunodeficient nature of the mice used, we could not analyze the role of EVs in immunity. Further investigation is warranted to determine whether pTDEs behave similarly in immunocompetent mice.
To generalize the inhibitory effect of primary tumors, we examined the difference in stemness between primary tumors and metastases. Then, we confirmed the difference in miRNA composition within EVs between the two groups. EVs derived from CD44+/CD24− populations of CIPp and CHMp cells have much greater antitumor activity than EVs derived from the whole CIPp cell population, suggesting that these CD44+/CD24− populations are involved in the suppression of metastases (Fig. 3).
Analysis of exosomal miRNAs revealed that miRNA-1 is abundant in primary tumor-derived EVs and that overloading miR-1 in pTDEs significantly enhances their growth-inhibitory effect (Figs. 4–5). Consistent with previous reports that miR-1 inhibits tumor growth and metastasis48, miR-1 is a tumor-suppressive miRNA conserved in humans and dogs that inhibits growth and metastasis in patients with breast cancer48. Furthermore, our study showed that treatment with pTDEs resulted in decreased expression of HACE1, which is a target of miR-1. Decreased expression of HACE1 led to the accumulation of Rac1, which in turn elevated ROS levels and induced cell cycle arrest. These findings are consistent with previous studies showing that HACE1 controls ROS generation by catalyzing the ubiquitination of Rac133. Taken together, these findings suggest that miR-1 is involved in the suppression of metastasis.
miR-1 expression in primary tumors is not confined solely to CMT, as evidenced by its detection in primary colorectal cancer samples obtained from human patients49. In human colorectal cancer cell lines, miR-1 expression was also greater in SW480 (primary tumor) cells than in SW620 (metastases) cells50. In addition, compared with SW620 cells, SW480 cells exhibit CD133+ stem-like characteristics51. Our findings in dogs provide a valuable basis for research in human patient-derived cell lines, given that miR-1 is conserved in both species. These consistent results between dogs and humans provide evidence of a relationship between primary tumors, CSCs, and miR-1. Notably, however, EVs contain not only miRNAs but also other nucleic acids, proteins, and lipids that might play roles in inhibiting metastases.
Next, we observed the intriguing potential of EVs to serve as biomarkers for both HBC and CMT owing to their ability to be detected in biological fluids. Notably, samples from subjects with HBC and CMT exhibited increased levels of exosomal miR-1, with a progressive increase observed in HBC patients with advancing TNM stage. We postulate that this difference may reflect the primary tumor’s augmented release of exosomal miR-1 upon DTC dissemination and colonization of distant organs. Furthermore, consistent with our clinical observations, we found that HACE1 gene expression was a predictor of inferior OS in patients with both primary tumors and metastases compared to patients with only primary tumors (Fig. 6).
Our study has several limitations. It is insufficient to make a generalize as we have only verified in canine mammary gland tumors without confirming it in various types of cancers. While we observed changes in the HACE1/Rac1 pathway upon miR-1 enrichment, we did not elucidate the direct molecular pathway responsible for the observed decrease in proliferation. Additionally, we did not clarify how the abundance of CSCs in primary tumors affects growth inhibition. In future studies, we plan to address these limitations by including other primary tumor and metastatic cell lines for comparison and conducting additional in-depth research to elucidate the role of exosomal miR-1. Nevertheless, our research has initiated a new era of exploration into the inhibitory effect of EVs originating from primary tumors on metastasis, laying the foundation for future studies in this field.
In summary, we have provided the first evidence that EVs secreted by CSCs in primary tumors can impede metastatic growth. Moreover, our investigations provide a molecular basis for the EV-mediated suppression of DTC proliferation. Specifically, pTDEs harboring miR-1 exert dual effects: they induce ROS-mediated genomic instability and cell cycle arrest in metastatic cells while also impeding angiogenesis in nearby endothelial cells. Furthermore, our findings highlight that the most crucial factor in inhibiting metastatic growth is miR-1, which, when overexpressed in pTDEs, has therapeutic potential. Additionally, the increase in exosomal miR-1 levels in the serum with the progression of breast cancer suggests its potential use as both a diagnostic marker and a therapeutic agent.
Data availability
All the raw and processed exosomal miRNA-seq data for the CHMp and CHMm cell lines and their biological replicates generated in this study have been deposited in the NCBI Gene Expression Omnibus (GEO) database under accession number GSE213969.
Code availability
The main code scripts used for miRNA-seq processing and visualization are described in detail and available at GitHub: https://github.com/snu-cdrc/exosomal-miRNA.
References
Riggio, A. I., Varley, K. E. & Welm, A. L. The lingering mysteries of metastatic recurrence in breast cancer. Br. J. Cancer 124, 13–26 (2021).
Giancotti, F. G. Mechanisms governing metastatic dormancy and reactivation. Cell 155, 750–764 (2013).
Park, S.-Y. & Nam, J.-S. The force awakens: metastatic dormant cancer cells. Exp. Mol. Med. 52, 569–581 (2020).
Borriello, L. et al. Primary tumor associated macrophages activate programs of invasion and dormancy in disseminating tumor cells. Nat. Commun. 13, 1–19 (2022).
Ji, Q. et al. Primary tumors release ITGBL1-rich extracellular vesicles to promote distal metastatic tumor growth through fibroblast-niche formation. Nat. Commun. 11, 1–18 (2020).
Piranlioglu, R. et al. Primary tumor-induced immunity eradicates disseminated tumor cells in syngeneic mouse model. Nat. Commun. 10, 1–13 (2019).
Phan, T. G. & Croucher, P. I. The dormant cancer cell life cycle. Nat. Rev. Cancer 20, 398–411 (2020).
Gimbrone, M. A. Jr, Leapman, S. B., Cotran, R. S. & Folkman, J. Tumor dormancy in vivo by prevention of neovascularization. J. Exp. Med. 136, 261–276 (1972).
Neophytou, C. M., Kyriakou, T.-C. & Papageorgis, P. Mechanisms of metastatic tumor dormancy and implications for cancer therapy. Int. J. Mol. Sci. 20, 6158 (2019).
O’Reilly, M. S. et al. Angiostatin: a novel angiogenesis inhibitor that mediates the suppression of metastases by a Lewis lung carcinoma. Cell 79, 315–328 (1994).
O’Reilly, M. S. et al. Endostatin: an endogenous inhibitor of angiogenesis and tumor growth. Cell 88, 277–285 (1997).
Willimsky, G. & Blankenstein, T. Sporadic immunogenic tumours avoid destruction by inducing T-cell tolerance. Nature 437, 141–146 (2005).
Théry, C., Zitvogel, L. & Amigorena, S. Exosomes: composition, biogenesis and function. Nat. Rev. Immun. 2, 569–579 (2002).
Tan, Y. et al. Tumor-derived exosomal components: the multifaceted roles and mechanisms in breast cancer metastasis. Cell Death Dis. 12, 1–18 (2021).
Syn, N., Wang, L., Sethi, G., Thiery, J.-P. & Goh, B.-C. Exosome-mediated metastasis: from epithelial–mesenchymal transition to escape from immunosurveillance. Trends Pharmacol. Sci. 37, 606–617 (2016).
Tian, W., Liu, S. & Li, B. Potential role of exosomes in cancer metastasis. BioMed Res. Int. 2019, 4649705 (2019).
Fu, Q. et al. Primary tumor-derived exosomes facilitate metastasis by regulating adhesion of circulating tumor cells via SMAD3 in liver cancer. Oncogene 37, 6105–6118 (2018).
Ono, M. et al. Exosomes from bone marrow mesenchymal stem cells contain a microRNA that promotes dormancy in metastatic breast cancer cells. Sci. Signal. 7, ra63–ra63 (2014).
Murai, K. et al. Establishment of a pair of novel cloned tumour cell lines with or without metastatic potential from canine mammary adenocarcinoma. Res. Veterinary Sci. 93, 468–472 (2012).
Zhang, D., Lee, H., Zhu, Z., Minhas, J. K. & Jin, Y. Enrichment of selective miRNAs in exosomes and delivery of exosomal miRNAs in vitro and in vivo. Am. J. Physiology-Lung Cell. Mol. Physiol. 312, L110–L121 (2017).
Abdelmegeed, S. M., & Mohammed, S. Canine mammary tumors as a model for human disease. Oncol. Lett. 15, 8195–8205 (2018).
Kim, T.-M. et al. Cross-species oncogenic signatures of breast cancer in canine mammary tumors. Nat. Commun. 11, 1–13 (2020).
Li, R., Huang, Y. & Lin, J. Distinct effects of general anesthetics on lung metastasis mediated by IL-6/JAK/STAT3 pathway in mouse models. Nat. Commun. 11, 642 (2020).
Duyverman, A. M., Steller, E. J., Fukumura, D., Jain, R. K. & Duda, D. G. Studying primary tumor–associated fibroblast involvement in cancer metastasis in mice. Nat. Protocols 7, 756–762 (2012).
Ben-Eliyahu, S. Tumor excision as a metastatic Russian roulette: perioperative interventions to improve long-term survival of cancer patients. Trends Cancer 6, 951–959 (2020).
Kim, M.-Y. et al. Tumor self-seeding by circulating cancer cells. Cell 139, 1315–1326 (2009).
Stark, G. R. & Taylor, W. R. Analyzing the G2/M checkpoint. Checkpoint Controls Cancer 280, 51–82 (2004).
Dankert, J. F. et al. Cyclin F-mediated degradation of SLBP limits H2A. X accumulation and apoptosis upon genotoxic stress in G2. Mol. Cell 64, 507–519 (2016).
Ayob, A. Z. & Ramasamy, T. S. Cancer stem cells as key drivers of tumour progression. J. Biomed. Sci. 25, 1–18 (2018).
Velasco-Velázquez, M. A., Popov, V. M., Lisanti, M. P. & Pestell, R. G. The role of breast cancer stem cells in metastasis and therapeutic implications. Am. J. Pathol. 179, 2–11 (2011).
Monteiro, J. & Fodde, R. Cancer stemness and metastasis: therapeutic consequences and perspectives. Eur. J. Cancer 46, 1198–1203 (2010).
Kuhn, N. Z. & Tuan, R. S. Regulation of stemness and stem cell niche of mesenchymal stem cells: implications in tumorigenesis and metastasis. J. Cell. Physiol. 222, 268–277 (2010).
Daugaard, M. et al. Hace1 controls ROS generation of vertebrate Rac1-dependent NADPH oxidase complexes. Nat. Commun. 4, 1–13 (2013).
Mettouchi, A. & Lemichez, E. Ubiquitylation of active Rac1 by the E3 ubiquitin-ligase HACE1. Small GTPases 3, 102–106 (2012).
Micalizzi, D. S., Maheswaran, S. & Haber, D. A. A conduit to metastasis: circulating tumor cell biology. Genes Develop. 31, 1827–1840 (2017).
Massagué, J. & Obenauf, A. C. Metastatic colonization by circulating tumour cells. Nature 529, 298–306 (2016).
Ganesh, K. & Massagué, J. Targeting metastatic cancer. Nat. Med. 27, 34–44 (2021).
Weiss, F., Lauffenburger, D. & Friedl, P. Towards targeting of shared mechanisms of cancer metastasis and therapy resistance. Nat. Rev. Cancer 22, 157–173 (2022).
Chen, W., Hoffmann, A. D., Liu, H. & Liu, X. Organotropism: new insights into molecular mechanisms of breast cancer metastasis. NPJ Precision Oncol. 2, 1–12 (2018).
Huang, Y. et al. Pulmonary vascular destabilization in the premetastatic phase facilitates lung metastasis. Cancer Res. 69, 7529–7537 (2009).
Rodrigues, G. et al. Tumour exosomal CEMIP protein promotes cancer cell colonization in brain metastasis. Nat. Cell Biol. 21, 1403–1412 (2019).
Ma, X., Wang, M., Yin, T., Zhao, Y. & Wei, X. Myeloid-derived suppressor cells promote metastasis in breast cancer after the stress of operative removal of the primary cancer. Front. Oncol. 9, 855 (2019).
Fidler, I. J. The pathogenesis of cancer metastasis: the’seed and soil’hypothesis revisited. Nat. Rev. Cancer 3, 453–458 (2003).
Rashid, O. M. et al. Resection of the primary tumor improves survival in metastatic breast cancer by reducing overall tumor burden. Surgery 153, 771–778 (2013).
Dauplat, J., Le Bouëdec, G., Pomel, C. & Scherer, C. in Seminars in surgical oncology. 42–48 (Wiley Online Library).
Colleoni, M. et al. Annual hazard rates of recurrence for breast cancer during 24 years of follow-up: results from the international breast cancer study group trials I to V. J. Clin. Oncol. 34, 927 (2016).
Demicheli, R., Retsky, M. W., Hrushesky, W. J. & Baum, M. Tumor dormancy and surgery-driven interruption of dormancy in breast cancer: learning from failures. Nat. Clin. Pract. Oncol. 4, 699–710 (2007).
Liu, C., Zhang, S., Wang, Q. & Zhang, X. Tumor suppressor miR-1 inhibits tumor growth and metastasis by simultaneously targeting multiple genes. Oncotarget 8, 42043 (2017).
Slater, C., de La Mare, J. A. & Edkins, A. L. In vitro analysis of putative cancer stem cell populations and chemosensitivity in the SW480 and SW620 colon cancer metastasis model. Oncol. Lett. 15, 8516–8526 (2018).
Xu, L. et al. Tumor suppressor miR-1 restrains epithelial-mesenchymal transition and metastasis of colorectal carcinoma via the MAPK and PI3K/AKT pathway. J. Transl. Med. 12, 1–12 (2014).
Wang, Y. et al. Gene expression profile of cancer stem‑like cells in the SW480 colon adenocarcinoma cell line. Oncol. Rep. 42, 386–398 (2019).
Acknowledgements
We thank Dr. Wan-Hee Kim, the College of Veterinary Medicine at Seoul National University, and Dr. Sun-Young Hwang at Haemaru Referral Animal Hospital for providing canine clinical specimens. We also thank Dr. Jeong Eon Lee and Dr. Soo-Youn Lee at the Samsung Medical Center for assistance in providing human clinical specimens. We would like to thank to the Organic and Biology Analysis Team of National Center for Interuniversity Research Facilities (NCIRF) at Seoul National University for their untiring efforts and perseverance.
Funding
This research was supported by grants from the Ministry of Science and ICT and the National Research Foundation of Korea (NRF) SRC program: Comparative Medicine Disease Research Center (CDRC) (2021R1A5A1033157).
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Writing – original draft: H.S.K. Writing – review & editing: J.Y.C. and K.H.L. Methodology: H.S.K., K.H.L. Mouse experimental methodology: H.S.K. and T.J.S. Bioinformatics methodology: K.H.S. Visualization: H.S.K. Conceptualization and study supervision: J.Y.C.
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Kim, CY., Lee, KH., Son, K.H. et al. Extracellular vesicle-mediated transfer of miRNA-1 from primary tumors represses the growth of distant metastases. Exp Mol Med 56, 734–746 (2024). https://doi.org/10.1038/s12276-024-01181-7
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DOI: https://doi.org/10.1038/s12276-024-01181-7