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Stable expression of fluorescently tagged proteins for studies of mitosis in mammalian cells

Nature Methods volume 2, pages 981–987 (2005)Cite this article

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Figure 1: Confocal imaging of LLC-PK-1 cells in mitosis.
Figure 2: Mitosis in living LLC-PK-1 cells expressing GFP-tubulin.
Figure 3: Imaging GFP-tubulin in siRNA-treated LLC-PK1-1α cells.

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Authors and Affiliations

  1. Department of Biology, University of Massachusetts, Amherst, Amherst, 01003, Massachusetts, USA

    Patricia Wadsworth, Nasser M Rusan, U Serdar Tulu & Carey Fagerstrom

  2. Program in Molecular and Cellular Biology, University of Massachusetts, Amherst, Amherst, 01003, Massachusetts, USA

    Nasser M Rusan & U Serdar Tulu

Authors
  1. Patricia Wadsworth
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  2. Nasser M Rusan
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  3. U Serdar Tulu
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  4. Carey Fagerstrom
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Corresponding author

Correspondence to Patricia Wadsworth.

Supplementary information

Supplementary Video 1

The movie sequence shows microtubules in an LLC-PK1-alpha cell in mitosis. The cell is shown from before nuclear envelope breakdown through cytokinesis. (MOV 19442 kb)

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Wadsworth, P., Rusan, N., Tulu, U. et al. Stable expression of fluorescently tagged proteins for studies of mitosis in mammalian cells. Nat Methods 2, 981–987 (2005). https://doi.org/10.1038/nmeth1205-981

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