Abstract
Reconstituting tissues from their cellular building blocks facilitates the modeling of morphogenesis, homeostasis and disease in vitro. Here we describe DNA-programmed assembly of cells (DPAC), a method to reconstitute the multicellular organization of organoid-like tissues having programmed size, shape, composition and spatial heterogeneity. DPAC uses dissociated cells that are chemically functionalized with degradable oligonucleotide 'Velcro', allowing rapid, specific and reversible cell adhesion to other surfaces coated with complementary DNA sequences. DNA-patterned substrates function as removable and adhesive templates, and layer-by-layer DNA-programmed assembly builds arrays of tissues into the third dimension above the template. DNase releases completed arrays of organoid-like microtissues from the template concomitant with full embedding in a variety of extracellular matrix (ECM) gels. DPAC positions subpopulations of cells with single-cell spatial resolution and generates cultures several centimeters long. We used DPAC to explore the impact of ECM composition, heterotypic cell-cell interactions and patterns of signaling heterogeneity on collective cell behaviors.
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Main
The functional properties of tissues arise through interactions of numerous cell types1. In vivo, these interactions occur in a three-dimensional (3D) setting in the context of specific tissue structures. Tissue structure—defined here as tissue size, shape, composition and spatial heterogeneity (i.e., the relative location of multiple cell types) and the surrounding ECM—serves to organize the exchange of chemical, electrical and mechanical information between neighboring and distant cells. An orderly exchange of signals allows cells to arrive at collective decisions and organize collective behaviors2. Defining the impact of a tissue's structure on the behavior of its constituent cells remains a major goal of developmental biology and is a requirement for the successful application of tissue engineering to regenerative medicine3. However, directly connecting tissue structure to collective cell behaviors remains challenging: tissue structure is difficult to alter in vivo, and the inherent structural complexity of tissues has so far precluded their de novo synthesis in vitro.
Current efforts typically focus on the narrower challenge of reconstituting specific elements of tissue structure in vitro. Common to all efforts is 3D cell culture, a requirement for proper tissue structure formation and cell behavior4. For example, 3D culture in mechanically and chemically defined ECM gels directs the morphogenesis of stem cells and cancer cells into organoids that model normal development and tumorigenesis, respectively5,6,7. However, rudimentary 3D culture methods lack key microenvironmental cues from surrounding tissue components that are necessary to specify tissue architecture over larger distances. Therefore, they provide limited control over ultimate tissue architecture. Dielectrophoretic patterning and micromolding have shown the effect of tissue size and shape on cell anabolic activity, differentiation, autocrine signaling, mechanics and tissue outgrowth8,9,10. However, dielectrophoresis is limited to conditions with low ionic strength, and micromolding cannot typically position multiple cell types in precise arrangements or use ECM formulations having physiological stiffness (<10 kPa), such as Matrigel. A variety of techniques have demonstrated that tissue composition, often referred to as cellular heterogeneity, contributes to a spectrum of collective cell behaviors absent from homogeneous tissues11,12,13. Finally, 3D printing techniques provide a potentially general strategy to reconstitute spatial heterogeneity for tissues with large features but suffer from low cell viability, can be limited in their ECM compatibility, and cannot efficiently build cell-dense tissues with spatial features the size of single cells14. Therefore, no generalizable methods are available for simultaneously reconstituting the size, shape, composition and spatial heterogeneity of tissues for long-term 3D culture.
To provide a rapid and modular means of reconstituting multiple aspects of tissue structure in 3D culture, we describe a bottom-up strategy that uses a DNA-patterned substrate15 as a template and temporary DNA-based cellular adhesions16,17,18,19 as synthetic linkages between cellular building blocks (Fig. 1a,b). DPAC is a modular method for controlling 3D microtissue structure across multiple length scales that can incorporate multiple cell types with high viability. We applied DPAC to study the impact of tissue size, shape, composition and spatial heterogeneity as well as the embedding ECM on individual and collective cell behaviors.
Results
To assemble an array of epithelial microtissues embedded in ECM gels, we proceeded through a series of steps (Fig. 1c and Supplementary Video 1) that begin with patterning a series of ∼7-μm amino-modified DNA spots on an aldehyde-coated glass slide using a BioForce Nano eNabler20,21. Reductive amination results in a covalent linkage between the DNA and the slide. A 180-μm-tall poly(dimethylsiloxane) (PDMS) flow cell was placed above the DNA pattern, allowing the addition of reagents and cell suspensions in a minimized (30-μl) volume. The slide was passivated to background cell binding by treatment with hydrophobic silane and blocking with albumin. In parallel, two populations of human mammary epithelial cells (MCF10A) were labeled for 5 min with a 5 μM solution of either a lipid-modified oligonucleotide or its complement17,22. The cells labeled with DNA complementary to the template were introduced to the flow cell and incubated for 5 min. Single cells adhered to single DNA spots. After gentle washing, a pattern of cells matching the pattern of DNA spots on the template was revealed. Iterating with alternating populations of complementarily labeled cells assembled hemispherical microtissues, layer-by-layer, upward and outward from the single cells (Supplementary Fig. 1). Addition of Matrigel containing DNase cleaved the DNA, releasing the array of microtissues into the supporting ECM gel as it set at 37 °C. Finally, the gel-encapsulated array was removed from the surface template, and an underlay of liquid ECM gel resulted in a seamless and fully embedded 3D culture upon gelation.
To more clearly illustrate the 3D embedding process, we assembled microtissue arrays through two rounds of DPAC but embedded the arrays in Matrigel containing covalently bound Alexa Fluor 555 and then underlaid the arrays with Matrigel containing Alexa Fluor 488. The unstained microtissues were observed at the interface of the two fluorescent gel layers. No voids were observed. The initially DNA-adherent cells were found to concentrate the gels at their surfaces over 24 h in culture as they rearranged and condensed into microtissues (Fig. 1d). Cell viability exceeded 97% (n = 613) through assembly, transfer to ECM gels and 24-h culture. Microtissues polarized their cytoskeletal and adhesion machinery appropriately, consistent with previous reports11,23 (Supplementary Fig. 1).
Precise DNA surface patterning provides the opportunity for precise spatial arrangement of large numbers of single cells, and whole microtissues, for fully embedded 3D culture. To quantify the capacity of DPAC to preserve spatial information when transferring patterns of cells from two dimensions to three dimensions, we first prepared arrays of DNA triangles with 18-μm or 38-μm edges. We used DPAC to render the DNA pattern as cells fully embedded in a Matrigel slab. Imaging of the embedded culture revealed a cell-to-cell spacing of 20 ± 2 and 40 ± 3 μm, respectively (mean ± s.d., n = 400; Fig. 2a and Supplementary Fig. 2). In another experiment, we varied cell spacing between two cell types in increments of several micrometers (Supplementary Fig. 3). To quantify the precision of cell positioning over larger distances and in less repetitive and more biologically inspired arrangements, we generated a bitmap pattern from a whole mount image of a mouse mammary fat pad. We used DPAC to render the image as a 1.6-cm pattern of over 6,000 single mammary epithelial cells fully embedded in Matrigel (Fig. 2b). The difference between cell positions on glass (2D) and embedded in Matrigel (3D) were visualized using a heat map (Fig. 2c,d). The majority of the differences occurred along the long, open axis of the flow cell (Supplementary Fig. 2). Expected cell-cell distances differed from actual cell-cell distances with a median of 22 μm across the whole pattern (n = 3.6 × 107 pairs) (Fig. 2e) and only 10 μm across cell pairs spaced less than 50 μm apart (n = 1.9 × 104 pairs) (Fig. 2f).
We found that DPAC is compatible with varied cell types and ECMs. Because cellular interactions are programmed with DNA, rather than genetically encoded adhesion molecules, the identity of the feedstock cells is arbitrary. For example, we successfully patterned primary or immortalized neuronal, epithelial, fibroblastic, endothelial and lymphocytic cells with high resolution and yield (Supplementary Fig. 1). The choice of matrices is limited only by what can be added to the cellular pattern as a liquid and subsequently gel under biocompatible conditions. Thus, we transferred patterns of cells to Matrigel, collagen, fibrin, agarose and their mixtures (Supplementary Fig. 1).
We also found that DPAC provides a flexible strategy for simultaneously controlling tissue size, shape, composition, spatial heterogeneity and ECM. We first demonstrated simultaneous control of tissue size and composition by showing that pairs of green and red fluorescent epithelial cells patterned closer than 18 μm apart condensed into single tissues upon transfer to Matrigel (Supplementary Fig. 3). Triangles comprising three uniquely stained epithelial cells behaved similarly (Fig. 3a). We prepared microtissues of equivalent size but different composition by performing multistep DPAC on cell triangles having two possible compositions (Fig. 3b,c). We prepared an array of over 700 microtissues containing a target of 8–13 cells total but either one or three fluorescent cells. For both compositions, 85% of microtissues contained the target number of total cells, and 79% of those microtissues also contained the target number of fluorescent cells. In comparison, the theoretical maximum yield for a Poisson-limited method, such as microwell molding, would be 26% or 16% for one or three fluorescent cells, respectively. We prepared larger microtissues by either increasing the area of the templating DNA pattern or further iterating layer-by-layer DPAC (Fig. 3d and Supplementary Table 1).
A unique capability of DPAC is its capacity to reconstitute cell-dense microtissues having tailored spatial heterogeneity. Unlike a printer, DPAC defines cell position by cell-cell connectivity rather than coordinates in a 3D Euclidean space. Therefore, the templating DNA pattern, and the order of addition of different DNA-functionalized populations of cells, determines the cell-cell connectivity of the assembled microtissue. To demonstrate this concept, we used DPAC to reconstitute microtissues consisting of three juxtaposed cellular compartments, with one compartment boundary in the xy plane and the other in the xz plane (Fig. 3e). This was accomplished using two orthogonal pairs of DNA sequences and a specific eight-step assembly scheme. We elaborated this strategy to form a microtissue having a core-shell topology similar to that of the human mammary gland. We assembled primary human luminal (LEP) and myoepithelial (MEP) cells using two orthogonal DNA sequences, a six-step DPAC scheme and a bull's eye–shaped templating pattern. When released from the template and fully embedded in Matrigel, the microtissue retained the programmed topology, which was reinforced after 24 h in culture (Fig. 3f). Some of these microtissues had lumenized by 72 h (Supplementary Fig. 3). We used a similar strategy to prepare arrays of either homogeneous or heterogeneous filled tubes of MCF10A cells having defined patterns of spatial heterogeneity (Fig. 3g,h).
DPAC provides a means to elucidate the effects of tissue structure on collective cell behavior by allowing simultaneous control of tissue size, shape, composition and spatial heterogeneity and the surrounding ECM. We explored this enabling capacity of DPAC in several model systems.
We first explored the impact of ECM composition on organoid tissue branching. We assembled microtissues from primary human luminal and myoepithelial cells; these microtissues were then embedded in either Matrigel or collagen I. Collagen I has previously been shown to influence the branching of mouse mammary organoids. Such organoids are prepared by mincing intact tissues, which yields a wide distribution of sizes and shapes24. To control for size and shape, we used DPAC to assemble similarly sized microtissues that were initially round upon transfer to Matrigel or collagen (Fig. 4a). After 24 h of culture, collagen-embedded microtissues had lower circularity (mean ± s.d. of 0.36 ± 0.13, n = 25) than Matrigel-embedded microtissues (mean ± s.d. of 0.73 ± 0.11, n = 25, P = 2.8 × 10−14, two-tailed Welch's t-test) (Fig. 4a,b). Qualitatively, the pattern of branching in collagen resembled those previously reported for randomly minced mouse mammary organoids24.
We next explored the impact of tissue size on cell growth rate. We reconstituted over 1,000 MCF10A (10A) microtissues ranging in size from 2 to 20 cells and tracked cell position over 72 h. Analysis of the growth trajectories of individual microtissues revealed that growth rate was inversely proportional to initial microtissue size. This trend was also observed for microtissues assembled from more rapidly dividing cells expressing oncogenic H-RasV12 (10AT), as well as for microtissues bearing mixed populations of 10As and 10ATs (Fig. 4d and Supplementary Fig. 4). Proliferation rates fit a generalized logistic growth model25.
We next explored the impact of tissue composition on the growth of single cells, but in microtissues of fixed size. We synthesized an array of 5- to 8-cell microtissues from mixed populations of 10As and 10ATs (Fig. 4c). As expected, microtissues grew more rapidly as the proportion of 10ATs in the tissue increased (Fig. 4d). Unexpectedly, the rate of microtissue growth did not appear to be a linear combination of the growth rates of the two cell populations. Further investigation revealed that 10ATs triggered a statistically significant increase in the growth rate of neighboring 10As (Fig. 4e) (mean 0.53 × 10−2 h−1, P = 0.04, one-way analysis of variance (ANOVA) with Holm-Sidak correction). Intriguingly, this effect appeared to require more than one 10AT cell in the tissue (P = 0.03, one-way ANOVA with Holm-Sidak correction) (Supplementary Fig. 4), suggesting that even small compositional differences can alter the rate of tissue growth through cell-cell interactions.
Finally, we explored the impact of defined spatial heterogeneity on branching morphogenesis. During the branching morphogenesis of a variety of tissues, gradients of growth factors trigger the activation of their receptors and downstream pathways in distinct patterns of spatial heterogeneity26,27,28,29. Whether the heterogeneous patterns of pathway activation are sufficient to trigger branching tissue outgrowth—or whether additional guidance cues from external gradients are required—has not been explored. Therefore, we used DPAC to synthesize filled tubes of 10As incorporating 10% 10ATs. As 10ATs express the Ras oncogene at low levels, they simulate a population of cells with chronic stimulation of their growth factor receptors11. The 10ATs were patterned randomly, in the middle or at the end of the 10A-filled tubes.
Cell dynamics and tissue morphology differed substantially between the three patterns over 72 h. We visualized changes in microtissue morphology by capturing single confocal slices from at least 12 microtissues from each cell pattern, which were combined to generate average intensity maps of the fluorescent 10A and 10AT nuclei (Fig. 4f). 10ATs in randomly patterned tubes comingled with 10As but also extruded basally or capped local protrusions, consistent with previous reports11,30 (Fig. 4f,g and Supplementary Fig. 4). However, 10ATs patterned in the middle of tubes translated outward laterally and formed filled acini-like structures. Similar results were observed for tubes incorporating 10% 10ATs at their ends. Branching occurred along all three axes (Supplementary Fig. 4). End-patterned microtissues showed a statistically significant increase in length at 72 h (mean ± s.d. of 371 ± 38 μm, n = 18, P = 7.6 × 10−6, two-tailed Welch's t-test) compared to microtissues in which the 10ATs were patterned in the center (mean ± s.d. of 319 ± 28 μm, n = 18). 90% intensity contours of 14 tissues indicated that the 10A component was also substantially longer in these tissues compared to the microtissues having 10ATs patterned centrally or randomly (Fig. 4h). We examined the 3D structure of these microtissues qualitatively by CLARITY31 (Fig. 4i,j and Supplementary Fig. 4). 10As formed necks connecting the filled 10AT-containing acini to the main 10A microtissue. Middle- and end-patterned microtissues showed evidence of lumenization.
In vivo, epithelial tissues are supported by a variety of stromal cells, including fibroblasts, adipocytes, lymphocytes and endothelial cells. Therefore, we explored the capacity of DPAC to reconstitute stromal cells into spatially organized 3D cultures. First, we prepared a branched pattern of endothelial cells (HUVECs) 5 mm long and fully embedded in a Matrigel-collagen mixture. After 24 h, the pattern condensed into a continuous network of endothelial cells and formed side branches (Fig. 5a and Supplementary Fig. 5). Immunofluorescence staining of fixed 72-h cultures provided evidence of phenotypic maturity, including vascular endothelial cadherin (VE-cadherin) localization to cell-cell junctions and exclusion from cell-ECM interfaces (Fig. 5b). To more closely mimic vasculature, we prepared microtissues of HUVECs with human brain vascular pericytes (HBVPs). At 72 h, immunofluorescence staining revealed a subset of HBVPs stably associated with the HUVECs and extending cellular processes among endothelial cell-cell junctions32 (Fig. 5b).
Second, we explored the impact of mural cells on the frequency and length of HUVEC side branches (Fig. 5c–e). After 24 h of culture, HUVECs branched with a frequency of 1.1 mm−1 (s.d. = 0.53, n = 7) and an average branch length of 58 μm (s.d. = 11 μm, n = 36) (Fig. 5a,d,e). Smooth muscle cells (SMCs) or HBVPs significantly increased the frequency (mean ± s.d. of 2.7 ± 0.4 mm−1, n = 9, P = 0.0017, two-tailed Welch's t-test, and mean ± s.d. of 2.3 ± 0.4 mm−1, n = 5, P = 0.0009, two-tailed Welch's t-test, for SMCs and HBVPs, respectively) (Fig. 5d) and the length (mean ± s.d. 89 ± 38 μm, n = 59, P < 0.0001, two-tailed Welch's t-test, and mean ± s.d. 94 ± 35 μm, n = 36, P < 0.0001, two-tailed Welch's t-test) of HUVEC side branches (Fig. 5e) when assembled superficially to the HUVEC cords. Mesenchymal stem cells (MSCs) decreased the frequency of side branches (mean of 0.041 mm−1, n = 9, P < 0.0001, two-tailed Welch's t-test), yielding endothelial networks with remarkably smooth edges (Fig. 5c,e).
Finally, we generated a variety of microtissues having multiple and distinct epithelial and stromal compartments. These microtissues incorporated endothelial networks, fibroblasts and epithelial cells using six-step DPAC and three orthogonal pairs of DNA sequences (Fig. 5f,g and Supplementary Fig. 5). After 48 h of culture in Matrigel-collagen mixtures, some microtissues with perpendicularly oriented fibroblasts and HUVEC compartments distorted, with HUVECs forming extensions proximal to the patterned fibroblasts (Fig. 5g). We resynthesized microtissue arrays consisting only of correspondingly oriented fibroblasts and HUVECs and measured increased extension of HUVECs into ECM near (mean ± s.d. of 103 ± 47 μm, n = 106) and far (mean ± s.d. of 85 ± 38 μm, n = 106, P = 1.4 × 10−3, one-tailed Welch's t-test) relative to the fibroblast compartment after 24 h of culture (Fig. 5h). These results demonstrate that the morphologies and behaviors of endothelial networks are altered by the proximity of networks of fibroblasts.
Discussion
There remain numerous opportunities for improving DPAC. For example, delivery of structured chemical, physical and hemodynamic signals to assembled microtissues, as well as the potential to perfuse embedded vasculature, could be achieved by merging DPAC with microfluidic technologies such as those used in organs-on-a-chip33. Merging DPAC with 3D printing could provide a means to control the spatial heterogeneity of ECM in addition to the spatial heterogeneity of cells. Combined with DPAC, folding, stacking34 or rolling35 techniques could generate thicker microtissues. Finally, the incorporation of stem cells or even whole tissue fragments as building blocks could enable the study of organoid development and disease processes in higher throughput and in a more reproducible 3D setting1. However, DPAC is fundamentally limited to cells or tissue components that can survive dissociation and that can be labeled by DNA. Given the capacity of DPAC to directly link complex tissue structural features with specific single and collective cell behaviors, we anticipate that this method will find utility in a variety of contexts, both basic and applied.
Methods
General materials and reagents.
Aldehyde-silanized glass slides (Nexterion Aldehyde AL, Schott), Sigmacote (Sigma-Aldrich), Sylgard 184 (Fisher Scientific), sodium borohydride (NaBH4, ACROS, 98%), Pluronic F108 NF (BASF), ethanol (Fisher Scientific), trypsin inhibitor from Glycine max (Sigma-Aldrich), Matrigel (BD Biosciences), rat-tail collagen 1 (BD Biosciences), Turbo DNase (Life Technologies), amine-modified ssDNA (5′-amine-X20, Operon), PBS (UCSF Cell-Culture Facility), PBS-CMF (UCSF Cell-Culture Facility), trypsin (UCSF Cell-Culture Facility), 100× penicillin-streptomycin, heat-inactivated FBS (UCSF Cell-Culture Facility), and RPMI medium (UCSF Cell-Culture Facility) were used as received without further purification. Lipid-modified ssDNA (5′-lipid-T80-X20) was synthesized as previously described17.
Cell culture.
MCF10A and MCF10AT cell lines were obtained from the Barbara Ann Karamanos Cancer Institute. Finite-lifespan HMECs and fibroblasts were provided by J.C.G. HUVECs, MSCs, and SMCs were purchased from Lonza. HBVPs were purchased from Sciencell. CAD cells were provided by K. Monahan. Bone marrow dendritic cells were provided by B. Boldajipour. Jurkats were purchased from the American Type Culture Collection.
MCF10A and MCF10AT cell lines were cultured as previously described23,36. Primary human mammary epithelial cells at passage 4 were established and maintained in M87A medium according as previously described37. CAD neuronal cells were cultured as previously described38. All other cells were cultured according to standard practices listed on American Type Culture Collection or Lonza.
No mycoplasma testing or cell authentication was performed for the experiments in this study.
Antibodies.
For immunofluorescence, the following antibodies were used: anti-human keratin 19 (Sigma cat. #C6930) (clone A53-B/A2) (1:50 dilution), anti-human keratin 14 (Thermo cat. #RB-9020-P) (polyclonal) (1:50 dilution), and anti-human CD49f (Millipore cat. #MAB1378) (clone GoH3) (1:50 dilution).
Preparation of PDMS flow cells.
Flow cells were cast with Sylgard 184 according to the specifications provided by Dow Corning. Briefly, the polymer and curing agent were mixed at a 10:1 ratio, degassed under vacuum, and cured over the flow-cell master at 70 °C. The master was prepared with no. 1–thickness coverslips (Fisher Scientific) cut to the dimensions of 4.5 mm × 18 mm and attached to double-sided tape (3M, cat. 665) of 0.0762-μm thickness. The final dimensions of the flow-cell master were 4.5 mm × 18 mm × 0.22 mm attached to a Nunclon (Fisher Scientific) Petri dish. Each PDMS flow cell was individually cut to have 1-mm-thick side walls and to have a 4.5-mm-wide inlet and outlet. Flow cells were treated with atmospheric plasma before use, as described below.
Preparation of DNA-patterned surfaces.
Cell and tissue patterns were designed as bitmap images in Microsoft Paint and translated into 6- to 8-μm-diameter droplets of 1.5 mM 5′-amine-modified ssDNA (5′-amine-X20, Operon) in a spotting solution of 225 mM NaCl, 22.5 mM sodium citrate, 5% (w/v) trehalose, 0.1 mg ml−1 N-octylglucoside, pH 9.5, onto aldehyde-silanized glass slides (Nexterion Aldehyde AL, Schott) via the BioForce Nano eNabler. Upon completion of printing, ssDNA patterns were baked at 120 °C for 15 min and then stored in a vacuum desiccator until use.
Patterned slides were reduced in a solution of 0.25% NaBH4 in 25% ethanol and 75% PBS for 15 min. Slides were washed twice with 0.1% SDS, washed three times with dH2O, and then air-dried. For silanization, 150 μl of Sigmacote (Sigma-Aldrich) were pipetted onto the slide and a coverslip placed on top. After 5 s, the coverslip was removed and the slide submerged into a tube of 50 ml absolute ethanol. The slide was inverted ten times and then transferred into a fresh tube of 50 ml absolute ethanol. The inverting was repeated, and the slide was transferred into a tube of dH2O for a final set of inversions. The slide was removed from the tube and dried under a stream of air. A flow cell was cut for each pattern on the slide, cleaned of dust with tape, and subjected to atmospheric plasma in a Plasma Etch PE-50 for 35 s under 200-mtorr pressure with 15 cc min−1 gas flow and at intermediate power. Flow cells were immediately positioned over the patterned slide and secured with gentle finger pressure. The flow cells were primed with a solution of RPMI-1640, 10% FBS, 63.7 mg l−1 penicillin G, 100 mg l−1 streptomycin sulfate, and 1% Pluronic F108. The solution was left in the flow cell for 5 min at room temperature to block the surface, and then the flow cell was equilibrated with four flow-cell volumes of calcium- and magnesium-free PBS (PBS CMF) and left undisturbed until ready for programmed assembly.
Preparation of DNA-labeled cells.
All cell-lines were labeled with lipid-ssDNA prepared according to a published procedure1. Briefly, cells were incubated for 5 min at room temperature with 5 μM of lipid-DNA. Sequences were chosen according to the requirements of each specific experiment. DNA-labeled cells were washed three times with PBS CMF and temporarily stored on ice until required for programmed assembly. DNA sequences are provided in Supplementary Table 2.
Programmed assembly and tissue embedding of cell patterns.
DNA-labeled cells were resuspended to a concentration of 107 cells ml−1, and 20 μl of this suspension were introduced to one end of the flow cell. Either the cells were allowed to settle to the surface by gravity for 5–10 min or the slide was centrifuged for 3 min at 8g in a Sorvall Legend RT+ centrifuge with acceleration and deceleration set to minimum. Ten flow-cell volumes of PBS CMF were introduced into the flow cell to wash out unhybridized cells. The procedures in this paragraph were repeated for each assembly step desired, taking 5–15 min for each successive assembly step.
Once the desired cell populations were assembled in the flow cell, a mixture of hydrogel precursor (for example, Matrigel) and DNase was introduced to the flow cell. One typical formulation was 6.1 mg ml−1 Matrigel, 2.1 mg ml−1 collagen I, 40 U ml−1 Turbo DNase, ice cold. Another typical formulation was 9.0 mg ml−1 Matrigel, 40 U ml−1 Turbo DNase, ice cold. The flow cell was put in an incubator at 37 °C for 30 min to allow for DNA cleavage and for the liquid gel to set as a solid hydrogel. Next, a border of 20 μl PBS CMF was applied all around the flow cell to reduce stiction, and then a sterile razor blade was used to slide the flow cell off the surface and onto a 20-μl droplet of hydrogel precursor waiting in a 3.5-cm culture dish. The dish was transferred to an incubator at 37 °C for 30 min to allow the underlying gel to set. 3 ml of prewarmed culture medium were added to the dish so as to completely submerge the flow cell. Sharp tweezers were used to carefully slide the flow cell off the set hydrogel. The released flow cell was then removed from the dish. The dish was returned to the incubator for culture.
Immunofluorescence.
All samples were fixed with 4% formaldehyde for 20 min and then incubated in blocking buffer (10% heat-inactivated goat serum in PBS+0.5% Triton X-100) at 4 °C for at least 1 d. Primary antibodies were then diluted in blocking buffer and added to the sample. After at least 1 d incubating at 4 °C with the primary antibodies, samples were washed several times with PBS+Triton X-100 for at least one day and incubated with fluorophore-conjugated secondary antibodies diluted at a concentration of 1:200 in blocking buffer for approximately 1 d. All sample were washed with PBS+1 μg ml−1 DAPI for at least 1 h before imaging.
Image acquisition.
All confocal microscopy images were acquired using a temperature-, atmosphere-, and humidity-controlled spinning-disk confocal microscope (Zeiss Cell Observer Z1 equipped with a Yokagawa spinning disk and running Zeiss Zen Software). All other images were acquired using an inverted epifluorescence microscope (Zeiss Axiovert 200M running SlideBook software).
Cell growth measurements.
Cell assemblies in 20 × 20 square arrays with pitch xy of 300 μm were imaged approximately every 24 h by driving the Zeiss Cell Observer spinning-disk confocal microscope to a preset list of nominal xy positions at 20× magnification with a z-slice spacing of 3 μm. Cell nuclei in red and green emission channels were counted manually from raw .tiff z stacks and maximum-intensity projection images. Growth rates for each assembly were calculated as the slope of plots of log2(N/No) vs. t where N is cell number at time t and No is initial cell number, assuming logarithmic growth of cells.
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Acknowledgements
The authors thank K. Monahan (University of California, San Francisco) for providing CAD cells, B. Boldajipour and the members of the Krummel lab (University of California, San Francisco) for providing bone marrow dendritic cells, J. Liu (University of California, San Francisco) for sharing MCF10A and derivative cell lines expressing H2B-fluorescent proteins, C. Mosher for technical help with the Nano eNabler, and M. Riel-Mehan for help with illustration. This work was supported by the Department of Defense Breast Cancer Research Program (W81XWH-10-1-1023 and W81XWH-13-1-0221 to Z.J.G.); US National Institutes of Health common fund (DP2 HD080351-01 to Z.J.G.); Sidney Kimmel Foundation; US National Science Foundation (MCB-1330864 to Z.J.G.); and University of California, San Francisco, Program in Breakthrough Biomedical Research. Z.J.G. is supported by the University of California, San Francisco, Center for Systems and Synthetic Biology (National Institute of General Medical Sciences Systems Biology Center grant P50 GM081879). A.C. was supported by the US Department of Defense through the National Defense Science and Engineering program.
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Z.J.G., N.Y.J. and M.E.T. conceived the study; Z.J.G., M.E.T., N.Y.J., M.C.C. and A.J.H. designed experiments; N.Y.J., M.E.T., A.C., A.J.H., M.C.C. and J.C.G. performed experiments; M.E.T., N.Y.J., M.C.C., A.J.H. and J.F. analyzed and interpreted the data; and Z.J.G., M.E.T., N.Y.J., M.C.C. and A.J.H. wrote the manuscript. M.A.L. and T.A.D. discussed and commented on the manuscript along with all other authors.
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A provisional patent application has been filed on the basis of this work. Z.J.G. is a member of the scientific advisory board of Adheren, a company that is commercializing cell-tethering technology.
Integrated supplementary information
Supplementary Figure 1 Programming the reconstitution of fully ECM-embedded 3D microtissues by DNA-programmed assembly (DPAC).
(a) Timeline for a three-component tissue synthesis by DPAC. The process proceeds through a series of steps taking less than eight hours. (b) An array of microtissues assembled above single cells through four synthetic steps. Detail and orthogonal views of a representative aggregate is shown to the right. (c) Photographs of three PDMS flow cells on a 75 mm glass slide. Arrowhead indicates cross-section of flow cell. (d) MCF10A microtissue arrays stained for polarity and cytoskeletal markers at two time points. (e) Phase contrast microscopy image of a grid of 7 μm-wide DNA droplets at 14 μm pitch. These droplets are sufficiently dense that hybridized epithelial cells (~16 μm diameter dotted blue lines) come into contact with one another. (f) Photograph of aqueous DNA droplets on an aldehyde-glass slide, showing the contact angle of the liquid on the silanized glass surface. (g) Schematic illustrating scoring for cell viability. A circle denotes a cell that has died after 24 hrs, an “x” denotes a microtissue that was ignored due to having more than one green cell at time zero. In (e), scale bars are 10 μm. All other scale bars are 100 μm.
Supplementary Figure 2 Cell position is preserved upon transfer of cell patterns from their template to ECM for fully embedded 3D culture.
(a) Fluorescence microscopy images of a two-component DPAC pattern comprising a millimeter-scale spiral composed of micro-scale green and red cell triangles. Inset at right shows detail. (b) Bitmap images (left column) and phase-contrast microscopy (center and right columns) of dense, curved, and fractal patterns assembled via a single round of DNA-mediated cell-surface adhesion. (c) Phase-contrast microscopy images of cell arrays in 3D gels. Clockwise from top-left: neurons, epithelial cells, leukocytes, and fibroblasts. (d) Observed cell-to-cell spacing (mean ± s.d.) compared to the spacing of printed DNA spots (grey background) (n=200). (e) Component analysis of error vectors, dividing each error into its perpendicular x and y components. The x axis corresponds to the direction of fluid flow through the flow cell during DPAC and gel transfer steps. (f) Schematic illustrating the analytical method used for determining errors in cell position after transfer from templating pattern to 3D gel. For each cell in the pattern: (i) align p0 to pn. (ii) Define a ring of inner radius ri and outer radius r0, with origin p0. (iii) Measure the set {|p0 - p1|, |p0 - p2|,..., |p0 - pn|}. (iv) Repeat this process for every pn in the microtissue. (v) Take the median of values within each ring to give the “error” for a given distance range. (g) Vectors representing displacements from expected positions of all cells (and detail) in the mammary fat pad pattern. Vectors are colored according to the magnitude of displacement (h) Fluorescence/phase-contrast microscopy image showing the full-field of a microtissue array constructed from three cell-type triangles. Arrowhead indicates computational stitching error introduced during image composition. (i) Phase-contrast microscopy of microtissues fully embedded in a variety of hydrogels. Clockwise from top-left: cells in agarose, Matrigel/collagen I mixture, Matrigel, collagen I, QGel, and fibrin. All scale bars are 100 μm.
Supplementary Figure 3 Reconstituting epithelial microtissues with programmed size, shape, composition, spatial heterogeneity and embedding ECM.
(a) Confocal fluorescence microscopy image with orthogonal views, showing LEP/MEP bilayer organization and lumenization for an HMEC organoid reconstituted via DPAC. (b) Fluorescence/phase-contrast microscopy images showing detail from a microtissue array incorporating cell pairs with nominal spacing between 12 and 26 μm in two micron increments. The frequency at which cell pairs coalesced into a single tissue is indicated below the images. (c) Confocal fluorescence microscopy images of MCF10As (green) and Chinese hamster ovary cells (red) assembled into a core-shell topology analogous to that shown in Fig. 3f. These microtissues do not retain their topology after 24 hr culture. (d) Cross-sectional view of the scheme used to synthesize the microtissues in Fig. 3f and S3a. (e) Fluorescence/phase-contrast microcopy images showing (left) detail of microtissues having similar total size but dissimilar minority cell composition. Rows (right) from microtissue arrays showing microtissues before and after 24 hr culture. (f) Maximum-intensity projection with orthogonal views of confocal fluorescence microscopy images illustrating a filled MCF10A tube. Arrows mark the 47 μm height of the tube. (g) Representative images of MCF10A cells assembled into cylindrical microtissues, shorter than those in Fig. 3h, and transferred to Matrigel/collagen mixtures. (h) Fluorescence/phase-contrast microscopy images showing MCF10A tubes having different patterns of spatial heterogeneity. In (c), scale bars are 30 μm. In (e), scale bars are 50 μm. All other scale bars are 100 μm.
Supplementary Figure 4 Measuring the impact of microtissue size, shape, composition and spatial heterogeneity and embedding ECM on collective cell behaviors.
(a) Plots of calculated growth rate compared to initial cell number for human mammary epithelial microtissues having four compositions of 10A and 10AT cells. (b) Time lapse microscopy showing growth trajectories for four representative microtissues. (c) Growth rate versus number of minority cells for heterotypic microtissues of the indicated composition (n = 66, 44, 27, 71). (d) Schematic illustrating analysis pipeline for extracting nuclei cell counts and positions, and reconstruction of growth trajectories for individual microtissues. (e) 3D reconstructions of heterotypic branching microtissues that have been cleared using CLARITY and imaged with confocal microscopy. Images from three viewing angles are shown. (f) Schematic illustrating analysis pipeline for the data presented in figure 4h. Fluorescence images are thresholded at 90 % intensity contours for (left) the sum of both channels, and (right) the red channel alone. The heights and widths of the total fluorescence 90% intensity contour was measured as indicated. (g) Representative fluorescence images showing MCF10A filled tubes incorporating MCF10ATs randomly, at their edges, or at their centers after 72 hr in culture. (h) Fluorescence microscopy showing three views of a microtissue at 50 micron z-intervals. Wedges indicate in-focus regions of the branching microtissues in each plane. (i) Full-field fluorescence image (and detail) showing a 3D microtissue array comprising MCF10A/MCF10AT microtissues having four different programmed compositions. Homotypic controls occupy leftmost columns. (j) Schematic illustrating the method used for measuring organoid circularity in figure 4b. Phase contrast images are thresholded, binned, and traced. All scale bars are 100 μm
Supplementary Figure 5 DPAC control of stromal architecture.
(a) Photograph showing HUVEC endothelial network embedded in a Matrigel/collagen mixcture. A penny is placed alongside the pattern for scale. (b) Fluorescence/phase-contrast microscopy images showing three representative microtissues showing three distinct behaviors: unchanged HUVEC networks, bending HUVEC networks, and branching HUVEC networks are shown at left, center, and right, respectively. Uncultured and cultured (24 hr) images are shown at the top and bottom, respectively. (c) Full-field phase contrast microscopy images of HUVEC networks at the indicated stage of DPAC and culture. (d) Full-field fluorescence/phase-contrast microscopy images showing three-compartment and multi-component microtissue arrays. (e) Schematic of the method used for measuring HUVEC extension. The 24 hr culture image is aligned with the initial cell pattern. The 24 hr culture image is divided into thirds, and the height of the central third (medial) and left third (lateral) are determined and compared to the same regions in the uncultured image. All scale bars are 100 μm.
Supplementary information
Supplementary Text and Figures
Supplementary Figures 1–5, Supplementary Tables 1 and 2 (PDF 2176 kb)
Supplementary Video of DPAC Process
In step 1, cells attach to strand A. In step 2, cells attach to strand B. In step 3, cells assemble onto the cells from step 1. Playback is 8x real-time speed. (MP4 8907 kb)
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Todhunter, M., Jee, N., Hughes, A. et al. Programmed synthesis of three-dimensional tissues. Nat Methods 12, 975–981 (2015). https://doi.org/10.1038/nmeth.3553
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DOI: https://doi.org/10.1038/nmeth.3553
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