Abstract
Cell detachment is central to a broad range of physiopathological changes, but there are no quantitative methods to study this process. Here we report programmed subcellular release, a method for spatially and temporally controlled cellular detachment, and present quantitative results of the detachment dynamics of 3T3 fibroblasts at the subcellular level.
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Acknowledgements
This work was supported by US National Institutes of Health (D.W. and P.C.S.), National Science Foundation (P.C.S.), Howard Hughes Medical Institution (D.W. and P.C.S.) and the Achievement Awards for College Scientists foundation (B.W.). We thank members of Wirtz and Searson laboratories for technical advice and reagents, S. Sun for useful discussions and H. Vo for assistance with microfabrication of devices.
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Supplementary Text and Figure
Supplementary Figures 1–5 and Supplementary Methods (PDF 11186 kb)
Supplementary Movie 1
Time lapse movie of sub-cellular release (see Fig. 1f). Magnification, 10×. (MOV 12174 kb)
Supplementary Movie 2
Time lapse movie of sub-cellular release (see Supplementary Fig. 2a). Magnification, 10×. (MOV 7983 kb)
Supplementary Movie 3
Time lapse movie of sub-cellular release (see Supplementary Fig. 2b). Magnification, 60×. (MOV 18930 kb)
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Wildt, B., Wirtz, D. & Searson, P. Programmed subcellular release for studying the dynamics of cell detachment. Nat Methods 6, 211–213 (2009). https://doi.org/10.1038/nmeth.1299
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DOI: https://doi.org/10.1038/nmeth.1299
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