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Reply to “Control of T cell activation by vitamin D”

Nature Immunology volume 12, pages 3–4 (2011)Cite this article

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Geisler replies:

The induction of VDR and PLC-γ1 takes place in both CD4+ T cells and CD8+ T cells after TCR stimulation1 and not only in CD4+ cells, as stated by Smolders et al. above. As for the availability of 25(OH)D3 and 1,25(OH)2D3 in the T cells in our experimental setup, it is known that the concentrations of 25(OH)D3 and 1,25(OH)2D3 in serum are around 100 nM and 80 pM, respectively. Most 25(OH)D3 and 1,25(OH)2D3 in serum is bound to the vitamin D–binding protein DBP2. We now have data that demonstrate endocytosis of DBP by T cells (data not shown). The intracellular concentrations of 25(OH)D3 and 1,25(OH)2D3 in freshly isolated T cells are therefore most likely equal to their concentrations in serum. In addition, according to the manufacturer, X-VIVO 15 medium contains human serum albumin and thus most probably also contains DBP3; both of these are sources of 25(OH)D3 and 1,25(OH)2D3.

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  1. Department of International Health, Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark

    Carsten Geisler

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Correspondence to Carsten Geisler.

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Geisler, C. Reply to “Control of T cell activation by vitamin D”. Nat Immunol 12, 3–4 (2011). https://doi.org/10.1038/ni0111-3b

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