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Double-stranded DNA arrays: next steps in the surface campaign

Nature Biotechnology volume 17, pages 536–537 (1999)Cite this article

It's only eight years since surface photoaddressable synthesis of oligonucleotides and oligopeptides was first devised1. But in that time, the technology has advanced with astonishing speed, addressing an ever-increasing array of applications, from expression analysis to genotyping and mutation screening. In this issue, Church and colleagues2 take DNA chips to another level, by synthesizing double-stranded DNA arrays accessible to proteins. Their system could allow the systematic study of DNA–protein interactions, transforming the analysis of eukaryotic transcriptional control.

Current synthetic oligonucleotide arrays typically consist of single-stranded 20- to 30-mers affixed to glass surfaces by oligoethylene glycol linkers. These arrays have many uses, such as monitoring the presence and abundance of mRNAs in populations3, assessing sequence variation in HIV-14, determining the relative fitness of yeast strains haploid at a given locus in a population of diploids5, and identifying the complete set of crossovers in individual meioses in yeast6.

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Figure 1: Alternative approaches for making double-stranded DNA arrays.

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  1. The Molecular Sciences Institute, 2168 Shattuck Avenue, Berkeley, 94704, CA

    Robert Carlson & Roger Brent

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  1. Robert Carlson
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  2. Roger Brent
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Carlson, R., Brent, R. Double-stranded DNA arrays: next steps in the surface campaign. Nat Biotechnol 17, 536–537 (1999). https://doi.org/10.1038/9837

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