Protein purification articles within Scientific Reports

Featured

• Article
  30 April 2024 | Open Access

  Polyphosphate and tyrosine phosphorylation in the N-terminal domain of the human mitochondrial Lon protease disrupts its functions

  • Nina Kunová
  • , Gabriela Ondrovičová
  •  & Vladimír Pevala

• Article
  01 September 2023 | Open Access

  Lactiplantibacillus plantarum as a novel platform for production and purification of integral membrane proteins using RseP as the benchmark

  • Sofie S. Kristensen
  • , Marie V. Lukassen
  •  & Geir Mathiesen

• Article
  31 July 2023 | Open Access

  Antibody binding of amyloid beta peptide epimers/isomers and ramifications for immunotherapies and drug development

  • Elizabeth R. Readel
  • , Arzoo Patel
  •  & Daniel W. Armstrong

• Article
  07 July 2023 | Open Access

  Novel methods of immunogenic antigen selection for serological diagnosis of Parelaphostrongylus tenuis infection

  • Jessie Richards
  • , Stephen Kania
  •  & Richard Gerhold

• Article
  26 July 2022 | Open Access

  A multifaceted strategy to improve recombinant expression and structural characterisation of a Trypanosoma invariant surface protein

  • Hagen Sülzen
  • , Jitka Votrubova
  •  & Sebastian Zoll

• Article
  31 May 2022 | Open Access

  Substantiation of propitious “Enzybiotic” from two novel bacteriophages isolated from a wastewater treatment plant in Qatar

  • Ramya Ramadoss
  • , Moza Al-Shukri
  •  & Annette Shoba Vincent

• Article
  10 November 2021 | Open Access

  ASKA technology-based pull-down method reveals a suppressive effect of ASK1 on the inflammatory NOD-RIPK2 pathway in brown adipocytes

  • Saki Takayanagi
  • , Kengo Watanabe
  •  & Hidenori Ichijo

• Article
  30 September 2021 | Open Access

  A new humanized antibody is effective against pathogenic fungi in vitro

  • Tomas Di Mambro
  • , Tania Vanzolini
  •  & Mauro Magnani

• Article
  18 August 2021 | Open Access

  The Penn State Protein Ladder system for inexpensive protein molecular weight markers

  • Ryan T. Santilli
  • , John E. Williamson III
  •  & Song Tan

• Article
  17 June 2021 | Open Access

  Molecular mechanism of calcium induced trimerization of C1q-like domain of otolin-1 from human and zebrafish

  • Rafał Hołubowicz
  • , Andrzej Ożyhar
  •  & Piotr Dobryszycki

• Article
  03 June 2021 | Open Access

  Purification of antibody fragments via interaction with detergent micellar aggregates

  • Gunasekaran Dhandapani
  • , Ellen Wachtel
  •  & Guy Patchornik

• Article
  22 February 2021 | Open Access

  Three-dimensional structure of human cyclooxygenase (hCOX)-1

  • Morena Miciaccia
  • , Benny Danilo Belviso
  •  & Antonio Scilimati

• Article
  09 February 2021 | Open Access

  Purification of anti-glycoconjugate monoclonal antibodies using newly developed porous zirconia particles

  • Tetsuya Okuda
  • , Katsuya Kato
  •  & Shinjiro Kasahara

• Article
  28 January 2021 | Open Access

  A set of simple methods for detection and extraction of laminarinase

  • Ananthamurthy Koteshwara
  • , Nancy V. Philip
  •  & Subrahmanyam Volety Mallikarjuna

• Article
  10 April 2020 | Open Access

  Structure-based engineering of anti-GFP nanobody tandems as ultra-high-affinity reagents for purification

  • Ziyue Zhang
  • , Yao Wang
  •  & Motoyuki Hattori

• Article
  25 March 2020 | Open Access

  Transport and kinase activities of CbrA of Pseudomonas putida KT2440

  • Larissa Wirtz
  • , Michelle Eder
  •  & Heinrich Jung

• Article
  04 March 2020 | Open Access

  Elevated CO₂ concentration affects the defense of tobacco and melon against lepidopteran larvae through the jasmonic acid signaling pathway

  • Qiang Zhang
  • , Wenting Dai
  •  & Jinxin Li

• Article
  26 November 2019 | Open Access

  Membrane directed expression in Escherichia coli of BBA57 and other virulence factors from the Lyme disease agent Borrelia burgdorferi

  • Karie E. Robertson
  • , Chloe D. Truong
  •  & Debra T. Hansen

• Article
  07 November 2019 | Open Access

  Direct, gabapentin-insensitive interaction of a soluble form of the calcium channel subunit α₂δ-1 with thrombospondin-4

  • Ehab El-Awaad
  • , Galyna Pryymachuk
  •  & Markus Pietsch

• Article
  16 August 2019 | Open Access

  A novel thermostable d-amino acid oxidase of the thermophilic fungus Rasamsonia emersonii strain YA

  • Yuya Shimekake
  • , Takehiro Furuichi
  •  & Shouji Takahashi

• Article
  16 August 2019 | Open Access

  Meloidogyne graminicola protein disulfide isomerase may be a nematode effector and is involved in protection against oxidative damage

  • Zhong-ling Tian
  • , Ze-hua Wang
  •  & Jing-wu Zheng

• Article
  04 July 2019 | Open Access

  Fractionated mitochondrial magnetic separation for isolation of synaptic mitochondria from brain tissue

  • W. Brad Hubbard
  • , Christopher L. Harwood
  •  & Patrick G. Sullivan

• Article
  30 May 2019 | Open Access

  Purified human anti-Tn and anti-T antibodies specifically recognize carcinoma tissues

  • Natacha Zlocowski
  • , Veronica Grupe
  •  & Fernando J. Irazoqui

• Article
  06 March 2019 | Open Access

  Mass Transfer of Proteins in Aqueous Two-Phase Systems

  • I. Kaplanow
  • , F. Goerzgen
  •  & G. Schembecker

• Article
  26 February 2019 | Open Access

  Mutations in the dimer interfaces of the dengue virus capsid protein affect structural stability and impair RNA-capsid interaction

  • Janaina Figueira-Mansur
  • , Estefania A. Aguilera
  •  & Ronaldo Mohana-Borges

• Article
  22 January 2019 | Open Access

  The neurotoxic secreted phospholipase A₂ from the Vipera a. ammodytes venom targets cytochrome c oxidase in neuronal mitochondria

  • Jernej Šribar
  • , Lidija Kovačič
  •  & Igor Križaj

• Article
  08 October 2018 | Open Access

  Extraction and liposome reconstitution of membrane proteins with their native lipids without the use of detergents

  • Irina A. Smirnova
  • , Pia Ädelroth
  •  & Peter Brzezinski

• Article
  23 July 2018 | Open Access

  Falling water ice affinity purification of ice-binding proteins

  • Chen Adar
  • , Vera Sirotinskaya
  •  & Ido Braslavsky

• Article
  19 March 2018 | Open Access

  High yield bacterial expression, purification and characterisation of bioactive Human Tousled-like Kinase 1B involved in cancer

  • Siddhant Bhoir
  • , Althaf Shaik
  •  & Sivapriya Kirubakaran

• Article
  08 December 2017 | Open Access

  Utilization of blood by-products: An in silico and experimental combined study for BSA usage

  • Fátima Arrutia
  • , Rebeca Fernández
  •  & Francisco A. Riera

• Article
  08 November 2017 | Open Access

  Production and Purification of Filovirus Glycoproteins in Insect and Mammalian Cell Lines

  • Elizabeth C. Clarke
  • , Amanda L. Collar
  •  & Steven B. Bradfute

• Article
  06 November 2017 | Open Access

  High-level heterologous production and Functional Secretion by recombinant Pichia pastoris of the shortest proline-rich antibacterial honeybee peptide Apidaecin

  • Ximing Chen
  • , Juan Li
  •  & Paul Dyson

• Article
  25 October 2017 | Open Access

  Binding patterns of homo-peptides on bare magnetic nanoparticles: insights into environmental dependence

  • Silvia A. Blank-Shim
  • , Sebastian P. Schwaminger
  •  & Sonja Berensmeier

• Article
  11 October 2017 | Open Access

  An integrated microfluidic system using a micro-fluxgate and micro spiral coil for magnetic microbeads trapping and detecting

  • Xuecheng Sun
  • , Zhu Feng
  •  & Yong Zhou

• Article
  14 September 2017 | Open Access

  Kinetic adaptation of human Myo19 for active mitochondrial transport to growing filopodia tips

  • Marko Ušaj
  •  & Arnon Henn

• Article
  15 August 2017 | Open Access

  RGD delivery of truncated coagulase to tumor vasculature affords local thrombotic activity to induce infarction of tumors in mice

  • Rana Jahanban-Esfahlan
  • , Khaled Seidi
  •  & Tahereh Javaheri

• Article
  21 February 2017 | Open Access

  A simple approach for preparation of affinity matrices: Simultaneous purification and reversible immobilization of a streptavidin mutein to agarose matrix

  • Sau-Ching Wu
  • , Chris Wang
  •  & Sui-Lam Wong

• Article
  05 December 2016 | Open Access

  Refined purification strategy for reliable proteomic profiling of HDL_2/3: Impact on proteomic complexity

  • Michael Holzer
  • , Sabine Kern
  •  & Gunther Marsche

• Article
  23 September 2016 | Open Access

  A novel affinity-based method for the isolation of highly purified extracellular vesicles

  • Wataru Nakai
  • , Takeshi Yoshida
  •  & Rikinari Hanayama

• Article
  27 July 2016 | Open Access

  Characterisation of a New Fungal Immunomodulatory Protein from Tiger Milk mushroom, Lignosus rhinocerotis

  • V. Pushparajah
  • , A. Fatima
  •  & R. L. H. Lim

• Article
  01 October 2015 | Open Access

  The recombinant expression and activity detection of MAF-1 fusion protein

  • Ping Fu
  • , Jianwei Wu
  •  & Jian Liu

• Article
  30 September 2015 | Open Access

  Extracellular vesicles are rapidly purified from human plasma by PRotein Organic Solvent PRecipitation (PROSPR)

  • Xavier Gallart-Palau
  • , Aida Serra
  •  & Siu Kwan Sze

• Article
  28 September 2015 | Open Access

  A peptide factor secreted by Staphylococcus pseudintermedius exhibits properties of both bacteriocins and virulence factors

  • Benedykt Wladyka
  • , Marcin Piejko
  •  & Paweł Mak

• Article
  10 July 2015 | Open Access

  Escherichia coli as host for membrane protein structure determination: a global analysis

  • Georges Hattab
  • , Dror E. Warschawski
  •  & Bruno Miroux

• Article
  05 April 2013 | Open Access

  Bacterial expression strategies for several Sus scrofa diacylglycerol kinase alpha constructs: solubility challenges

  • Elizabeth J. Petro
  •  & Daniel M. Raben