Abstract
Ferroptosis, a newly discovered irondependent form of regulated cell death caused by excessive accumulation of lipid peroxides, is linked to the development and treatment response of various types of cancer, including gastric cancer (GC). Noncoding RNAs (ncRNAs), as key regulators in cancer, have both oncogenic and tumor suppressive roles. However, studies on ferroptosis-related ncRNA networks in GC are still lacking. Here, we first identified 61 differentially expressed genes associated with ferroptosis in GC by computing and analyzing gene expression profile of tumor and normal tissues for GC. Then, upstream lncRNAs and miRNAs interacting with them were found through miRNet and miRBase databases, and hub lncRNAs and miRNAs were obtained through topological analysis. Finally, the ceRNA regulatory network linked to ferroptosis in GC was established, which includes two ferroptosis marker genes (TXNIP and TSC22D3), one driver gene (GABARAPL1), and one suppressor gene (CAV1). Kaplan-Meier survival analysis showed that changes in the expression of these genes were associated with the survival of GC patients. Furthermore, our study revealed that this ceRNA network may influence the progression of GC by regulating ferroptosis process. These results will help experimental researchers to design an experiment study to further explore the roles of this regulatory network in GC ferroptosis.
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Introduction
Ferroptosis, a newly discovered mechanism and unique modality of programmed cell death, is driven by the accumulation of a large number of iron, free radical production, fatty acid supply, lipid peroxidation, and the destruction of intracellular redox balance1,2. It is an irondependent manner of nonapoptotic cell death. Many cellular biological processes can alter cellular susceptibility to ferroptosis by altering cellular iron contents3. For example, enhancing intracellular iron export makes some cells more resistant to ferroptosis4,5. A study suggests that when transferrin expression is silenced, hepatocytes compensatorily upregulate SLC39A14 expression, resulting in excessive import of iron that then leads to ferroptosis6. Additionally, numerous signaling pathways and cellular metabolic pathways relevant to human disease, notably cancer, are driven by ferroptosis7,8. A growing number of studies have confirmed that ferroptosis has been involved in the development of a variety of cancer, and plays a critical role in inhibiting tumorigenesis2,9. However, research into ferroptosis have just begun in many ways, and the underlying mechanism of ferroptosis in cancers, particularly gastric cancer, is poorly understood.
Gastric cancer (CG) is a common and deadly human malignancy that seriously threatens the lives and health of millions of people worldwide10,11. With the rapid development of medical technology, the diagnosis and treatment of GC have made great progress, but patient survival is still poor12,13. Precision therapy remains a challenge. More recently, accumulating evidence indicates that ferroptosis is critical for eliminating cancer cells, which could open up a new way for cancer therapy9,14.
Interestingly, long noncoding RNA (lncRNA) and microRNA (miRNA) are increasingly recognized as crucial regulators in the regulation of ferroptosis of cancer cells9,15. The regulatory loop between lncRNA and miRNA plays a dynamic role in transcription and translation of protein-coding genes, influencing multiple biological processes in cancers, such as cell death, cell cycle and proliferation16–18. lncRNAs function as competitive endogenous RNAs (ceRNAs) to regulate mRNAs through competitively binding miRNAs, therefore forming largescale regulatory networks across the transcriptome17. CeRNA regulatory networks play important roles in cancer initiation and development19,20,21, as well as have significant regulatory effects on the ferroptosis of cancer22. lncRNA NEAT1, as a ceRNA, facilitates ferroptosis in hepatocellular carcinoma by controlling miR-362-3p and MIOX23. MiR-375 can trigger ferroptosis to suppress the stemness of GC cells through interacting SLC7A11, which could be used as a potential target to induce ferroptosis24. However, the function and molecular mechanism of ncRNAs in regulating ferroptosis of cancers remain unclear.
To understand the relationship between ncRNAs and ferroptosis in gastric cancer, we constructed a ceRNA network related to ferroptosis, which revealed the underlying mechanism of lncRNAs and miRNAs in regulating ferroptosis of GC. First, aberrantly expressed genes associated with ferroptosis were obtained in GC by transcriptome analysis. Further, functional and pathway enrichment analyses were conducted to explore the roles of these genes in GC. Then, upstream lncRNAs and miRNAs that affected the abnormal expression of ferroptosis-related genes were identified, and the four key lncRNAs and six miRNAs were found by topological analysis. Finally, these four key lncRNAs were revealed to act as ceRNAs to modulate ferroptosis in gastric cancer by regulating cancer-related miRNAs and protein-coding genes. Collectively, our findings provide new insights into the regulation of ferroptosis as a mean of eliminating gastric cancer cells.
Results
Identification of ferroptosis-related genes in gastric cancer
Based on transcriptome data of gastric cancer from the TCGA Cohort, we analyzed significantly differentially expressed genes between the 343 tumor and 30 normal tissues using the negative binomial distribution. The 10,658 unique genes were identified. Among them, the 61 ferroptosis-related genes are dysregulated in GC (Fig. 1A and Table.SI in Supplementary Information 2). 22 of these 61 genes are upregulated (padj < 0.05 and log2FoldChang > 1) and 39 genes are downregulated in gastric cancer (padj < 0.05 and log2FoldChang < (−1); Fig. 1B). Their expression in 343 tumor and 30 normal tissue samples is shown in Fig. 1C,D.
In order to further understand the biological behavior of these 61 differentially expressed ferroptosis-related genes in GC, we performed the pathway and biological process enrichment analysis, such as KEGG Pathway, WikiPathways, GO Biological Processes, Reactome Gene Sets, and Canonical Pathways, by Metascape25 (Fig. 2 and Table.S1). The result showed that ferroptosis pathway is the most representative enriched term, with Log10 (p) equals (−16.00) and Log10 (q) equals (−11.65) (Fig. 2A), which including CAV1, TXNIP, DPP4, SLC1A5, TFRC, NOX1, and NOX4 (Table.S1). It has been indicated that NOX4 elevation can promote ferroptosis of astrocyte by activating oxidative stressinduced lipid peroxidation and impairing mitochondrial metabolism in Alzheimer's disease26 In addition, ferroptosis pathway was revealed to be closely related to multiple biological processes, such as positive regulation of cell death, fatty acid metabolic process, and reactive oxygen species metabolic process (Fig. 2B). Moreover, Chemical carcinogenesisreactive oxygen species and VEGFA-VEGFR2 signaling pathway were enriched. These data suggested that the abnormal expression of these 61 genes is associated with ferroptosis in gastric cancer, and is involved in other biological processes and signaling pathways related to tumor.
Identification of upstream lncRNAs and miRNAs associated with ferroptosis
According to the information provided by the FerrDb database, 22 of the 61 ferroptosis-related genes were identified as driver genes that can promote ferroptosis, such as MAPK3 and GABARAPL1; 14 genes were considered as suppressors, which can prevent ferroptosis, such as CDKN1A and CAV1; 33 genes were known as markers that can indicate the occurrence of ferroptosis, such as NFE2L2 and TXNIP (Fig. 3A). Among them, some genes play multiple roles in ferroptosis, with 6 genes in both drivers and markers, and 2 genes in both suppressors and markers (Fig. 3B).
In order to understand the role of ncRNAs in the ferroptosis of gastric cancer, we predicted upstream lncRNAs and miRNAs that interact with 61 ferroptosis-related genes. Based on the miRNet and miRBase databases, thousands of upstream miRNAs were found. Among them, 242 miRNA are significantly differentially expressed between tumor and normal tissues from GC (p < 0.01 and |log2FoldChang|> 1; Table.SII in Supplementary Information 3).These 242 miRNAs can bind to 57 ferroptosis-related genes, forming 992 interaction pairs. A hub subnetwork consisting of 22 differentially expressed miRNAs (DEmiRNAs) and 28 ferroptosis-related genes was constrcuted using the topological methods in CytoHubba (Fig. 3C). These DEmiRNAs and ferroptosis-related genes have a relatively high degree and play a key role in the network. The top 15 key DEmiRNAs and genes with higher degrees were listed, such as CDKN1A, TXNIP, and miR13p (Fig. 3E).
Additionally, the upstream lncRNAs associated with ferroptosis were predicted through the miRNet database. 433 lncRNAs were found to interact with 20 of the 22 DEmiRNAs. Among these lncRNAs, 83 lncRNAs are significantly differentially expressed between tumor and normal tissue from GC patients (padj < 0.05 and |log2FoldChang|> 1; Table.SIII in Supplementary Information 4). Based on the interaction between the 20 DEmiRNAs and 83 differentially expressed lncRNAs (DElncRNAs), a hub lncRNAmiRNA subnetwork was constructed by topological method. The network consists of 30 DElncRNAs and 20 DEmiRNAs (Fig. 3D). The top 15 key DElncRNAs and DEmiRNAs were displayed as in Fig. 3F. These key DElncRNAs and DEmiRNAs may play important roles in ferroptosis of gastric cancer.
Association between ferroptosis-related genes and survival of gastric cancer
To explore whether these ferroptosis-related genes affect the survival of GC patients, we performed Kaplan-Meier survival analysis. For the 28 key ferroptosis-related genes in hub network, seven genes were found to be associated with patient survival (p < 0.05; Fig. 4). The results showed that the five-year survival rate of SLC1A5 high expression group is higher than that of the low expression group, whereas the five-year survival rate of high expression group of other six genes is lower than that of the low expression group (Fig. 4). For example, patients with high expression of RGS4 and TXNIP exhibited a poorer prognosis in gastric cancer, while those with high expression of SLC1A5 have a better prognosis within five years.
Construction of ferroptosis-related ceRNA network in gastric cancer
Based on the ceRNA hypothesis, lncRNAs/mRNAs, as ceRNA molecules, function through miRNA response element (MRE) to competitively bind with same miRNAs, thereby regulating gene expression to affect cell function17,27. We analyzed the correlation between the expression of seven survival-related genes and DEmiRNAs from the hub subnetwork (Fig. 3C), through the Pearson correlation coefficient. The miRNA-mRNA interaction pairs with Corr < 0.5 and p < 0.05 were selected, which include the 8 miRNAs and 4 ferroptosis-related genes (Fig. 5A and Table S2).
Furthermore, Pearson correlation coefficient between the expression of these 8 miRNAs and DElncRNAs from the hub subnetwork (Fig. 3D) was calculated. lncRNA LINC00641, SNHG14, PWAR6, and PART1 showed negative correlation with the 8 miRNAs, six of which were statistically significant correlated to these lncRNAs (p < 0.05; Fig. 5C and Table S3). Moreover, these four lncRNAs interacted with the six miRNAs. In addition, these four lncRNAs were significantly positively correlated with the four ferroptosis-related genes (p < 0.05; Fig. 5B).
According to the above analysis, a ferroptosis-related ceRNA regulatory network was constructed by Cytoscape (Fig. 6), which contributes to understand the regulatory role of ncRNAs in ferroptosis of gastric cancer. In this network, the six key DEmiRNAs are shared by four key DElncRNAs (LINC00641, SNHG14, PWAR6, and PART1) and ferroptosis-related genes (CAV1, TXNIP, GABARAPL1, and TSC22D3). Moreover, the expression of these four DElncRNAs and four ferroptosis-related genes is significantly lower in GC than in normal tissues (Fig.S1). Conversely, the expression of six DEmiRNAs is markedly downregulated in GC tissues (Fig.S2). These data suggested that the four key ferroptosis-related genes are regulated by four key lncRNAs and six key miRNAs, thereby affecting ferroptosis in gastric cancer.
Validation of a potential ceRNA regulatory network associated with ferroptosis in gastric cancer
In the ferroptosis-related ceRNA regulatory network (Fig. 6), we found that TXNIP, a marker of the occurrence of ferroptosis, can be regulated through the interaction of lncRNA PWAR6 and miR-106b-5p. To further assess the reliability of the results, we analyzed the expression of TXNIP and lncRNA PWAR6 using GSE79973 GC dataset from the GEO database, as well as the expression of shared miR-106b-5p using GSE78091 GC dataset. These data indicated that the expression of TXNIP and PWAR6 is significantly reduced in GC tissues compared to normal tissues, and the expression of miR-106b-5p is markedly elevated in GC (p < 0.05; Fig. 7A). The target sites in TXNIP and PWAR6 were predicted to pair with miR-106b-5p by TargetScan analysis (p < 0.05; Fig. 7B). These results further demonstrate that lncRNA PWAR6, as a competitive endogenous RNA, affects the expression of TXNIP by competitively binding miR-106b-5p, which plays a critical role in the ferroptosis of GC.
Discussion
Ferroptosis is a recently emerged irondependent nonapoptotic cell death cascade that can eliminate cancer cells in a nonapoptotic manner, and is considered a key target for the development of anticancer therapies7,28. However, the mechanism that regulates ferroptosis remains unclear. Therefore, the discovery of key factors regulating ferroptosis in cancer has great clinical implications.
With recent advances in research into cancer biology, accumulating studies have shown that ncRNAs, especially lncRNAs and miRNAs, are important mediators in the regulation of ferroptosis and iron metabolism29,30. In this study, we obtained 22 upregulated and 39 downregulated genes associated with ferroptosis in gastric cancer, and identified the upstream DElncRNAs and DEmiRNAs that interact with these genes. For example, SLC1A5 was found to be an upregulated gene associated with ferroptosis in GC and related to patient prognosis (Fig. 1 and 4), which is consistent with the study of Xiang et al.31. Moreover, both our work and the study by Xiang et al. showed that hsa-miR-125b-5p can target SLC1A5 (Fig. 3), which may play an important role in the targeted therapy of GC. Besides SLC1A5, our study also found that 6 of the 61 abnormally expressed ferroptosis-related genes were associated with the prognosis of GC, which include CAV1, GABARAPL1, TSC22D3, PRKAA2, RGS4, and TXNIP (Fig. 4). The study further revealed that TXNIP, CAV1, GABARAPL1, and TSC22D3 may play key roles in the regulation of ferroptosis in GC. TXNIP, a metabolic protein, has been considered to be a tumor suppressor gene in various malignant tumors, and its overexpression can suppress the growth and metastasis of cancer cells in tumor transplant models32. TXNIP is downregulated in GC than in normal tissues and has been shown to be a key marker for the prognosis of patients with gastric cancer33. We confirmed similar results by multiple statistical methods and KM analysis. However, to our knowledge, few studies have explored ceRNA regulatory network related to ferroptosis.
The ceRNA regulatory networks play important roles in the initiation and progression of cancer34. Here we identified a key ferroptosis-related ceRNA regulatory network comprising 4 lncRNAs, 6 miRNAs, and 4 ferroptosis-related genes in gastric cancer. This study found that PWAR6, LINC00641, SNHG14, and PART1 are markedly downregulated and involved in ferroptosis of gastric cancer. Similarly, Yang et al. indicated that LINC00641 is underexpressed in glioma cells, its overexpression inhibits cell proliferation but promoted apoptosis, and functions as a ceRNA in glioma cells by absorbing miR-4262 to regulate NRGN35. Moreover, we found that these four lncRNAs are regulated by six key miRNAs, such as miR-106b-5p, miR175-p, and miR-200b-3p, which are involved in the regulation of ferroptosis and possibly serve as candidate biomarkers for the prognosis and treatment of gastric cancer. Although all of these results provided evidence for the roles of these four key lncRNAs and six miRNAs in ferroptosis, more experimental studies are needed to confirm their mechanisms in gastric cancer.
Conclusions
In conclusion, we analyzed the relationship between ncRNAs (lncRNAs and miRNAs) and ferroptosis in gastric cancer from the perspective of bioinformatics, and found an important ferroptosis-related ceRNA regulatory network, key lncRNAs and miRNAs that play critical roles in CG progression and affect the prognosis of GC patients. Hence, it will be important to validate the molecular mechanisms of these key lncRNA and miRNA regulators in ferroptosis of GC by experimental methods in the future. Our findings will provide references for proposing new biomarkers/targets of cancer therapy based on ferroptosis.
Methods
Data collection
RNA sequencing (RNAseq) data, miRNA sequencing (miRNAseq) data and corresponding clinical data of gastric cancer were collected from The Cancer Genome Atlas (TCGA) database (https://gdcportal.nci.nih.gov/). In this study, we downloaded the RNAseq data (raw counts and FPKM) of gastric adenomas and adenocarcinomas that contained the 343 tumor and 30 normal samples. FPKM is fragments per kilobase of exon model per million mapped fragments, reflects normalized gene expression level, and was transformed by log2. miRNA sequencing data of 410 tumor and 42 normal samples were obtained, which included the count data and normalized RPM (reads per million miRNA mapped reads) data. RPM values represent miRNA expression levels. In addition, GSE79973 and GSE78091 independent GC datasets were obtained from the GEO database (https://www.ncbi.nlm.nih.gov/geo/) and used as validation sets to validate the results of TCGA dataset analysis. GSE79973 dataset was generated using the GPL570 platform (Affymetrix Human Genome U133 Plus 2.0 Array) and normalized by the MAS 5.0 algorithm in GeneSpring Software 11.0 (Agilent Technologies, Santa Clara, CA, US). To further verify the reliability of our results, GEPIA, a web server for analyzing the gene expression profiling of 9,736 tumor and 8,587 normal samples from TCGA and GTEx projects36, was used to verify the expression of lncRNA and gene.
Differential expression analysis of ferroptosis-related genes
FerrDb is a manually curated database for experimentally validated regulators and markers of ferroptosis and ferroptosisdisease associations37. The 259 ferroptosis-related genes were obtained from FerrDb database. To identify ferroptosis-related genes that are differentially expressed between tumor and normal tissues from GC patients, R package “DESeq2” was used38. The raw count of RNAseq data was used as input data in the DESeq2 package. Adjusted P values (padj) by false discovery rate (FDR) and log2 Fold Change (log2FoldChang) were used as screening parameters for differentially expressed genes.
Prediction of ncRNAs interacting with ferroptosis-related genes
In order to obtain the upstream lncRNAs and miRNAs interacting with ferroptosis-related genes, miRNet, TargetScan and miRBase databases were used in this study39,40,41. These databases are widely applied in ncRNA studies, and their results have high confidence.
Identification of key ncRNAs and construction of hub ceRNA regulatory network
The regulatory network between ncRNAs and ferroptosis-related genes were constructed by Cytoscape software platform42. The CytoHubba plugin in Cytoscape was utilized to identify hub ferroptosis-related genes in the network and construct hub subnetwork. The degrees of ferroptosis-related genes/ncRNAs in the network were calculated by topological methods, such as Degree, MCC, MNC, and clustering coefficients. The higher the degree, the more important the genes/ncRNAs.
Kaplan-Meier survival analysis
Kaplan-Meier (KM) survival analysis was conducted to analyze the effect of ferroptosis-related gene expression on the survival of patients with GC. The gastric cancer samples were divided into high expression and low expression groups according to the median value of ferroptosis-related gene expression across all tumor samples. The log-rank test was applied to evaluate the difference in overall survival between the two groups of patients.
Statistical analysis
Most of analyses were performed using R software for statistical computing and graphics. Based on Pearson's correlation coefficient, the correlation between expression level of miRNA and mRNA in GC was calculated using the miRNAseq and RNAseq data of GC in TCGA database. Similarly, the correlation between expression level of lncRNA and mRNA/miRNA was calculated. The expression levels of mRNA and lncRNA are log2-transformed FPKM values. The expression level of miRNA is log2-transformed RPM value. The p < 0.05 was considered statistically significant.
Data availability
The datasets used and/or analyzed during the current study available from the corresponding author on reasonable request.
Abbreviations
- GC:
-
Gastric cancer
- ncRNA:
-
Nonconding RNA
- lncRNA:
-
Long noncoding RNA
- miRNA:
-
MicroRNA
- ceRNA:
-
Competitive endogenous RNA
- MRE:
-
MiRNA response element
- DElncRNA:
-
Differentially expressed lncRNA
- DEmiRNA:
-
Differentially expressed miRNA
- DEG:
-
Differentially expressed gene
- RPM:
-
Reads per million miRNA mapped reads
- KM analysis:
-
Kaplan-Meier analysis
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Funding
This work was financially supported by Natural Science Foundation of Inner Mongolia (No. 2022QN06007); the National Natural Science Foundation of China (No. 81960449); the Science Research Foundation of Inner Mongolia People’s Hospital (No. 2021YN18); the Science and Technology Planning Project for Health of Inner Mongolia (No. 202202024); and the Talent Training Plan for the Key Laboratory of Inner Mongolia Science and Technology Department.
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W.J. contributed to the design, bioinformatics analysis, drafting and writing of the manuscript. J.Y., Z.F. (Zongqi Feng) and N.H. devoted the work of negotiation and revising the manuscript. Z.F. (Zhenxing Feng), T.Y. and J.L. performed the statistical analysis. L.Y. provided full guidance of the study. All authors read and critically revised the manuscript for intellectual content and approved the final manuscript.
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Jin, W., Liu, J., Yang, J. et al. Identification of a key ceRNA network associated with ferroptosis in gastric cancer. Sci Rep 12, 20088 (2022). https://doi.org/10.1038/s41598-022-24402-3
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DOI: https://doi.org/10.1038/s41598-022-24402-3
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