Abstract
Ribosome biogenesis and turnover are processes necessary for cell viability and proliferation, and many kinds of proteins are known to regulate these processes. However, many questions still remain, especially in the Archaea. Generally, several ribonucleases are required to process precursor rRNAs to their mature forms, and to degrade rRNAs for quality control. Here, we found that FAU-1, which is known to be an RNA binding protein, possesses an RNase activity against precursor 5S rRNA derived from P. furiosus and T. kodakarensis in the order Thermococcales in vitro. An in vitro analysis revealed that UA sequences in the upstream of 5S rRNA were preferentially degraded by addition of FAU-1. Moreover, a fau-1 gene deletion mutant of T. kodakarensis showed a delay of exponential phase, reduction of maximum cell number and significant changes in the nucleotide sequence lengths of its 5S, 16S, and 23S rRNAs in early exponential phase. Our results suggest that FAU-1 is a potential RNase involved in rRNA stability through maturation and/or degradation processes.
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Introduction
FAU-1 (P. furiosus AU binding 1) is a protein that was first identified in Pyrococcus furiosus, a member of the order Thermococcales in the Euryarchaea. The protein is highly conserved in Thermococcales (shown in Fig. S1) and is speculated to be a potential orthologue of E. coli ribonuclease E (RNase E) which is one of the enzymes responsible for ribosomal RNA (rRNA) processing. FAU-1 has been characterized as an RNA-binding protein that binds to AU-rich sequences1. The amino acid similarity between FAU-1 and the N-terminal region of RNase E is approximately 25%1, indicating very low similarity. Furthermore, the catalytically active center of the RNase E/G domain in FAU-1 was not predicted by the protein families database (Pfam, http://pfam.xfam.org)2; however, the S1 domain and a partial region of the RNase E/G domain were predicted in FAU-1 (Fig. S1). The S1 domain is an RNA-binding region and the partial ribonuclease E/G domain corresponds to the 5′ sensor region involved in the recognition of the RNA substrates of RNase E3,4. However, it has not yet been demonstrated whether FAU-1 possesses RNase activity or not.
All organisms have developed quality control systems for the regulation of rRNAs, in which ribonucleases play integral roles. rRNA is initially transcribed as a precursor rRNA that undergoes multiple processing steps, including cleavage by various RNases, to generate mature rRNAs. RNases also participate in rRNA quality control by rapidly degrading aberrant rRNAs5,6,7, which is a well-studied process, particularly in bacteria. It has been reported that the RNase E/G family proteins are among the most important RNases responsible for rRNA maturation and quality control8,9,10,11,12. RNase E recognizes the 5′ end of single-stranded RNA and has endoribonuclease activity13,14. In Escherichia coli, RNase E is involved in the processing of precursor 5S rRNA and 16S rRNA, and is also associated with the stability of mature 16S rRNA and 23S rRNA9,15,16. RNase E requires either Mg2+ or Mn2+ ions for its efficient RNase activity and depends on a Zn2+ ion to form a functional tetramer3,17,18,19.
In recent studies, several RNases related to rRNA biogenesis have been identified in the Archaea. For example, the Nob1 enzyme is reported to process the 3′ end of the precursor 16S rRNA (pre-16S rRNA) in Pyrocuccs horikoshii (Euryarchaea, Thermococcales)20. However, the reports of rRNA processing in Archaea are still extremely limited compared with those for the Bacteria. Importantly, Thermococcales consists of hyperthermophilic archaea, thriving in conditions over 50 °C with enriched metal ions, and is thus dramatically different from bacteria21. Moreover, the components of the rRNA operon differ across archaeal species. For example, H. volcanii (Euryarchaea, Halobacteriales) has two 16S–23S–5S rRNA operons, whereas P. furiosus has a single 16S–23S rRNA operon and two independent 5S rRNA genes (Fig. 1A). Therefore, rRNA biogenesis has not yet been clarified in the Archaea.
The aim of the present study is to elucidate whether the FAU-1 possesses an RNase activity because of the partial similarity between FAU-1 and RNase E. The similarity of the regions involved in RNA recognition in FAU-1 and RNase E suggests that the mechanism that allows FAU-1 to recognize its target substrates might be similar to that of RNase E. To test this hypothesis, we purified recombinant FAU-1 protein, and assessed the RNA degradation activity using precursor 5S rRNA (pre-5S rRNA) which is known to be a substrate of RNase E. The in vitro results show the pre-5S rRNA is degraded by FAU-1 addition in a time and dose dependent manner. Furthermore, to investigate the roles of FAU-1 in vivo, we constructed fau-1 gene deletion mutant strains of Thermococcus kodakarensis, which had significant growth defects with different lengths of rRNAs. We propose that FAU-1 is a novel RNase which possesses an activity to degrade and/or process precursor rRNA.
Results
Sequencing analysis of 5′ precursor 5S rRNA sequences in P. furiosus
In E. coli RNase E, the S1 subdomain and 5′ sensor subdomain are crucial for its recognition of RNA3,4. Because each of these subdomains is predicted by the Pfam database (http://pfam.xfam.org) to be present in P. furiosus FAU-1 (Fig. S1A), we reasoned that P. furiosus FAU-1 might recognize the substrates of RNase E. We focused on pre-5S rRNA, one of the major RNA substrates of RNase E. The P. furiosus genome contains two 5S rRNA genes (PF_r003 and PF_r004) (Fig. 1A), but the pre-5S rRNA sequences have not yet been identified in a number of Archaea, including P. furiosus. To identify the P. furiosus pre-5S rRNA sequences, a cDNA library was prepared from P. furiosus RNA, size-fractionated to approximately 120–500 nucleotides (nt), and was sequenced using a 5S-rRNA-specific primer, as described in the Experimental procedures. Hereafter, we refer to P. furiosus or T. kodakarensis rRNA as “pfu XX rRNA” (e.g., pfu 5S rRNA) or “tko XX rRNA” (e.g., tko 16S rRNA), respectively, and to P. furiosus or T. kodakarensis protein as “pfu XXX” or “tko XXX”, respectively. The sequencing data indicated the presence of 63- and 54-nt sequences corresponding to the upstream regions of the PF_r003 and PF_r004 genes, respectively (Fig. 1B). These sequences were considered to be the 5′ precursor sequences of 5S rRNA. We then compared the nucleotide sequences of the pfu 5′ pre-5S rRNAs with those of upstream regions in the 5S rDNA in closely related species. We found an AU-rich sequence 27–39 nt upstream from the mature pfu 5S rRNA that was highly conserved in members of the Thermococcales, including Thermococcus and Pyrococcus (Fig. 1C). In P. furiosus, two promoter regions (approximately 30 and 60 nt upstream from the mature 5S rRNA coding site) were predicted in each 5S rRNA gene with the UCSC Archaeal Genome Browser database (http://archaea.ucsc.edu)22,23. Our results suggest that pre-5S rRNA is transcribed from a promoter approximately 60 nt upstream from the mature 5S rRNA coding site.
pre-5S rRNA is degraded by the reaction with FAU-1 in vitro
To test whether P. furiosus FAU-1 has RNase activity for pre-5S rRNA, we performed an in vitro degradation assay using purified pfu FAU-1 protein from fraction 10, shown in Fig. 2A (lane 3), and the pfu 5′ precursor 5S rRNA (186 nt). The RNA includes the mature 5S rRNA sequence (PF_r003, 126 nt) and the 60 nt upstream sequence. Hereafter, we refer to this 5′ precursor 5S rRNA substrate as the “pfu 5′ pre-5S rRNA”. To eliminate RNase and metal ion contaminations derived from E. coli, the purified FAU-1 was heated for 15 min at 85 °C, and was dialyzed twice with the buffer including 1 mM EDTA and 7 mM 2-mercaptoethanol in the process of purification (see Experimental Procedures). The transcribed RNA was purified on a denaturing polyacrylamide gel containing urea, and pfu 5′ pre-5S rRNA was excised and extracted from the gel. To mimic physiological conditions, the pfu 5′ pre-5S rRNA substrate was incubated at 65 °C in the presence or absence of pfu FAU-1, after which the samples were subjected to a northern blot analysis. The data show that the degradation of pfu 5′ pre-5S rRNA occurred in the presence of pfu FAU-1, and the degree of degradation correlated with the amount of pfu FAU-1 present (Fig. 2B and C, lanes 3–5, single asterisk). Degradation products of pfu 5′ pre-5S rRNA accumulated with increasing amounts of pfu FAU-1 (Fig. 2B and C, lanes 3–5, double asterisks). Despite this, no band corresponding to the exact size of mature pfu 5S rRNA was detected in samples treated with pfu FAU-1 (Fig. 2B, lanes 3–5, double asterisk, and lane 9), suggesting that other proteins are required to reproduce the complete maturation of 5S rRNA seen in vivo. The short degradation products detected with increasing amounts of pfu FAU-1 suggest that pfu FAU-1 has endoribonuclease activity, degrading 5′ pre-5S rRNA at various locations within its sequence (Fig. 2B, vertical line). Note that a slight increase in the 100-nt 5S rRNA degradation product was observed with heating over time and/or in the bovine serum albumin (BSA) control samples (Fig. 2B and C, lanes 1–2, and 6–8). However, the degree of degradation was not dependent on the level of BSA protein, suggesting that some nonspecific degradation occurred under these assay conditions. We also applied a higher temperature condition of 80 °C, and the results showed that pre-5S rRNA was degraded by FAU-1. On the other hand, experiments carried out under a lower temperature condition of 37 °C did not show any degradation activity (Fig. S2B and C). Additionally, we tested whether FAU-1 has degradation preferences of pre-5S rRNA using substrates containing pre-5S rRNA and yeast mature tRNA (Fig. S2D and E). The data suggested that the FAU-1 preferentially degrade pre-5S rRNA more than yeast tRNA control. The apparent degradation velocity of pfu FAU-1 against pre-5S rRNA is ~0.09 pmol/min/μg protein in this condition.
To determine whether FAU-1 has similar activity in other closely related species, we purified the FAU-1 protein from T. kodakarensis (Fig. 2A, lanes 6–10) and tested its activity in the in vitro degradation assay, as for pfu FAU-1. The precursor 5S rRNA probe substrate (186 nt), which included the mature 5S rRNA (Tk_r01, 126 nt) and its 60-nt upstream sequence, was transcribed and incubated in the presence or absence of tko FAU-1. The data show that the tko pre-5S rRNA decreased in a tko-FAU-1-dependent manner at 52 °C, but no processed rRNA species were detected with this protein (Fig. 2D and E, lanes 3–5). We also performed the same experiment at different temperature conditions, and degradation by tko FAU-1 was observed within a 46–65 °C temperature range (Fig. S2F and G). The apparent degradation velocity of tko FAU-1 against pre-5S rRNA is ~0.13 pmol/min/μg protein in this condition. The results raise the possibility that FAU-1 recognizes the pre-5S rRNA as a substrate for degradation in the Thermococcales.
FAU-1 preferentially cleaves UA sequences in pre-5S rRNA
To investigate the site-specificity of the pfu FAU-1 activity for pre-5S rRNA, a short RNA probe (73 nt) corresponding to the pre-5S rRNA was labeled at its 5′ end with fluorescein isothiocyanate (FITC) and incubated with pfu FAU-1 at 65 °C in vitro. Specific FAU-1 degradation products were consistently observed, cleaved at UA sequences in the probe, generating fragments ending in U (Fig. 3; U6, U10, U13, U17, U25, and U34), suggesting that pfu FAU-1 has UA-sequence-specific degradation activity. However, the residue immediately 5′ to the mature 5S sequences is an A, suggesting that additional activity may be required in addition to FAU-1 for 5S rRNA maturation. It is noted that some of the shorter fragments in the alkaline-fragmented control lane migrated as doublets (Fig. 3, lane 2). One possibility regarding this finding is due to a terminal 2′,3′-cyclic phosphate at the 3′ end of RNAs after degradation by FAU-1. It is consistent with reports that a terminal 2′,3′-cyclic phosphate at the 3′ end of RNAs is generated by some RNases, such as RNase I. Typically, RNAs with a terminal 2′,3′-cyclic phosphate migrate faster than the linear phosphate species on a denaturing gel24,25. Importantly, the pfu FAU-1 degradation products cleaved at positions U6 or U10 (Fig. 3, lanes 7–9) migrated faster than those in the negative control (Fig. 3, lanes 4–6 and 10–12). To test this hypothesis, we investigated whether bacterial alkaline phosphatase (BAP) treatment affects the electromobility of degradation products by FAU-1 or not. As a result, the electromobility of degradation products by FAU-1 did not change by phosphatase treatment, suggesting that FAU-1 degrades the pre-5S RNA sequences with formation of 2′,3′-cyclic phosphate and 5′hydroxyl termini (Fig. S3A and B).
Addition of Mg2+ ions promote the first-cut of pre-5S rRNA by FAU-1
A divalent metal ion is typically required for RNase activity, and the activity of RNase E is reportedly dependent on Mg2+ and/or Mn2+ ions17,19. A Zn2+ ion is also required for RNase E to form a tetrameric structure, and consequently indirectly affects its catalytic activity18. Therefore, we tested whether these divalent metal ions affect the pfu FAU-1 RNase activity for pre-5S rRNA in vitro. In this experiment, the addition of Mg2+ to pfu FAU-1 caused additive effects for degradation activity for pre-5S rRNA compared with treatment with pfu FAU-1 alone or Mg2+ alone (Fig. 4A and B, lanes 1–4). Note that strong degradation of the pre-5S rRNA was observed with the addition of Mn2+ or Zn2+ ions alone, but not with Mg2+ alone (Fig. 4A and B, lanes 6 and 9). The addition of Mn2+ to pfu FAU-1 weakly enhanced its RNase activity for pre-5S rRNA compared with the addition of only pfu FAU-1 or only Mn2+ (Fig. 4A and B, lanes 6–7, and Fig. S4). However, we could not evaluate whether FAU-1 and the Mn2+ or Zn2+ ions acted in a coordinated manner in the degradation of pre-5S rRNA, because the RNA probe was rapidly degraded by the Mn2+ or Zn2+ ions (Fig. 4A and B, lanes 6–7 and 9–10, and Fig. S4). It has been reported that Mn2+ or Zn2+ ions have nonspecific degradation capacities for RNA, and this could affect the stability of pre-5S rRNA26,27. We also performed the degradation assay under the same conditions with ethylenediaminetetraacetic acid (EDTA), a chelating agent for divalent metal ions. The degradation capacity of FAU-1 for pre-5S rRNA in the presence of EDTA was comparable to that of FAU-1 alone (Fig. 4A and B, lanes 1–5). To analyze the effects of Mg2+ on FAU-1 activity in more detail, we examined the degradation of pre-5S rRNA after the titration of Mg2+ or the FAU-1 protein. The degradation of pre-5S rRNA by FAU-1 increased in an Mg2+- or FAU-1-dependent manner (Fig. 4C and D). However, the main product was slightly reduced in the presence of Mg2+ ions compared to FAU-1 alone (Fig. 4C and D). Also, the pattern of accumulation of degradation products changed in the presence of the Mg2+ ion (Fig. 4C and D, vertical line). The apparent degradation velocity of pfu FAU-1 with 1 mM of Mg2+ ions against pre-5S rRNA is ~0.12 pmol/min/μg protein and it is higher than that with no addition of Mg2+ ions. These data suggest that the effect of Mg2+ ions for RNase activity of FAU-1 is enhancing the first-cut of 5′ pre-5S rRNA and changing the cutting pattern.
Degradation activity for pre-5S rRNA reduced in a series of FAU-1 mutants
Our Pfam analysis (http://pfam.xfam.org) predicted three domains, including the RNase E/G domain, in pfu FAU-1 (Fig. S1). The amino acid identity between the predicted RNase E/G domain in E. coli and that in pfu FAU-1 is 7%, whereas the similarity is approximately 23%. To test whether the RNase E/G domain of pfu FAU-1 is related to its RNase activity, plasmids expressing three histidine (3×His)-tagged FAU-1 were constructed in which pfu FAU-1 amino acid residues 170–189 (FAU-1-Δ170–189–His), 190–209 (FAU-1-Δ190–209–His), or 210–229 (FAU-1-Δ210–229–His) were deleted (Fig. 5A). These internally deleted regions are parts of the predicted pfu FAU-1 RNase E/G domain and are highly conserved in closely related species (Fig. S1). The recombinant mutant proteins were purified using the same procedure of wild-type FAU-1 (Fig. 5B), and the effects of the internal deletions were determined using the in vitro degradation assay. The RNA degradation activities of the internally deleted FAU-1 mutants were reduced compared with that of WT FAU-1 (Fig. 5C), and some of the smaller degradation products produced by WT FAU-1 were not produced by the internally deleted FAU-1 mutants (Fig. 5C, degradation products). However, pre-5S rRNA processing was still observed after treatment with all the variant proteins. Importantly, it should be noted that the FAU-1 mutants displayed changes in activity although the purification processes were identical, suggesting that the RNase activities observed in our experiments were due to the FAU-1 protein(s), and not contaminating proteins from E. coli. Here, we propose that pfu FAU-1 possesses an RNase activity, but which differs in characteristics to those from RNase E of E. coli. Conserved regions in the RNase E/G domain are related to its RNase activity, though a core domain involved in its RNase activity may be located in other parts.
Deletion of the fau-1 gene in T. kodakarensis showed that FAU-1 plays critical roles in cell viability and rRNA maturation
To clarify the role of FAU-1 in vivo, we deleted the fau-1 gene in T. kodakarensis, a species closely related to P. furiosus and for which the genome is easy to manipulate and tools well-established28,29. Among the Δfau-1 strains, growth defects were observed at 93 °C in two independent gene disruptants (Fig. 6A, KUW1, Δfau-1-n6 and Δfau-1-n11). Since rRNA production or stability are major factors in proliferation ratios, and our in vitro data suggested the relationship between FAU-1 and rRNA processing or degradation, we hypothesized that the cause of the growth defects was due to the rRNA status in fau-1 deletion mutant. To test this hypothesis, we extracted the RNA samples from KUW1 and Δfau-1 cells that were cultured and their growth was monitored as the optical density at 660 nm (OD660). The cells were harvested at OD660 0.05, 0.1, and 0.2, and the total RNA was extracted. The rRNA, normalized to the total RNA amount, was separated by electrophoresis on a denaturing agarose gel, and was detected with SYBR Green II staining. The electromobility of the 16S and 23S rRNAs differed between the KUW1 and Δfau-1 cells (Fig. S5A). To eliminate the effects of contaminants in a more detailed analysis, the 5S, 16S, and 23S rRNAs were purified with denaturing gel electrophoresis and then separated with a bioanalyzer. Consistent with the SYBR Green II analysis, the electromobility of the rRNAs from the Δfau-1 cells were significantly altered relative to that in the KUW1 cells (Fig. 6B–G and Table S1). The difference in electromobility of 23S rRNA at OD660 0.05 corresponded to a length difference of approximately 50 nt between the KUW1 and Δfau-1 cells (Fig. 6F–G, Table 1). In addition, we investigated the differences in degradation products of 16S and 23S rRNA between WT and Δfau-1 strains. As a result, the degradation patterns, sizes and accumulation levels were different between WT and Δfau-1 strains (Fig. S5B). These results show that FAU-1 is critical for T. kodakarensis cell growth in vivo and suggests that FAU-1 has a role in the maturation or stability of rRNAs.
Discussion
In a previous study, we found that P. furiosus FAU-1 is an RNA-binding protein1. Although pfu FAU-1 binds AU-rich sequences, the function of the FAU-1 protein was unclear. In this study, we found that FAU-1 possesses RNase activity against pre-5S rRNA (Fig. 2B–C) and specifically degrades UA sequences of pre-5S rRNA that are highly conserved in Thermococcales (Figs 1 and 3). RNase E also degrades pre-5S rRNA15 and has degradation specificity for UA sequences30,31. These characteristics may explain the similarities between the RNA recognition domains of pfu FAU-1 and E. coli RNase E (Fig. S1). Further, Mg2+ ion affects RNase activity of pfu FAU-1 against pre-5S rRNA, but details remain unclear (Fig. 4). Moreover, our result show that internal deleted FAU-1 reduce their RNase activity (Fig. 5), but the catalytic center of RNase activity of pfu FAU-1 is unknown (Fig. S1). To elucidate those questions, it will require X-ray crystal structural analysis of FAU-1 in the future.
The electromobility of 5S rRNA processed by FAU-1 in vitro differs from that of mature 5S rRNA of P. furiosus generated in vivo (Fig. 2B). Therefore, we infer that some factors are still lacking in vitro that are required to generate mature 5S rRNA. In Archaea, various modifications occur at several positions in the mature 5S and 23S rRNAs32,33,34,35. An rRNA methylation enzyme is also associated with the stability and maturation of rRNA in E. coli 36,37. The pre-5S rRNA transcribed in vitro in this study was not modified by those enzymes, and their absence may explain the differences in the electromobility of the pfu 5S rRNA processed by FAU-1 in vitro and that of the mature pfu 5S rRNA generated in vivo. Other factors that act cooperatively with FAU-1 may also be lacking in the in vitro experiments. For example, E. coli RNase E forms a huge protein heterocomplex, called the RNA degradosome, consisting of RhlB RNA helicase, enolase, PNPase, and Hfq, which work in concert to degrade RNA substrates38,39,40. We did not detect any products when we added tko FAU-1 for in vitro degradation assay (Fig. 2D). In addition to the reasons mentioned above, the amino acid identity of pfu FAU-1 and tko FAU-1 is 74.5% and this difference may produce different activities in in vitro assay. Indeed, the optimal reaction temperature in in vitro degradation assay is different between pfu and tko FAU-1 (Fig. S2).
Our results show that FAU-1 preferentially cleaves UA sequences in vitro, and the migration of the pfu FAU-1 degradation products cleaved at the U6A7 and U10A11 positions were different from those of no protein or BSA control (Fig. 3). When we digested the RNA probe with NaOH, we observed that doublet bands were evident on the gel at the lower molecular weights (Fig. 3, lane 2). We infer that the difference in the two bands is attributable to the propensity of the 3′ end of a phosphate group to cyclize. The lower band represents a degradation product with a 3′-terminal 2′,3′-cyclic phosphate, whereas the upper band represents a degradation product with a linear phosphate24. Moreover, the electromobility of degradation products by FAU-1 did not change by phosphatase treatment (Fig. S3A). This result suggests that the FAU-1 possesses the RNase activity with formation of 2′,3′-cyclic phosphate and 5′hydroxyl termini, as do RNase I and the tRNA splicing endoribonuclease EndA41,42,43.
To clarify the roles of FAU-1 in vivo, we constructed and analyzed fau-1 gene deletion mutants in T. kodakarensis. We observed delayed cell growth and a reduced maximal OD in the Δfau-1 mutant strains compared with those of the KUW1 strain (Fig. 6A). The electromobility of the 5S, 16S, and 23S rRNAs also differed between the KUW1 and Δfau-1 cells at OD660 0.05 (Fig. 6B–G and Table S1). For example, the electromobility of the Δfau-1 23S rRNA displayed a length approximately 50 nt greater than that in the KUW1 cells (Fig. 6F–G and Table S1). The reason for the difference in rRNA electromobility between the KUW1 and Δfau-1 cells has not been determined, but could potentially be explained by the effects of either rRNA processing and/or modification. However, our data suggest that the deletion of the fau-1 gene affects rRNA maturation, resulting in a growth defect from the lag phase to the stationary phase. Interestingly, the differences of rRNA length at OD660 0.05 disappeared at later points, and then cell proliferation started with a delay, suggesting that deletion of the fau-1 gene might be compensated by some different mechanisms, allowing cell proliferation in vivo.
The successful construction of the fau-1 gene deletion mutants indicates that the fau-1 gene is not essential for T. kodakarensis cell viability. This also suggests that alternative factors are involved in 5S rRNA maturation, beside FAU-1. It has been reported in H. volcanii (Euryarchaea, Halobacteriales) that the maturation of the 5′ end of the 5S rRNA can be performed by tRNase Z44, whose predominant role is in tRNA maturation. tRNase Z is thought to recognize the tRNA-like structure in the region upstream from the 5S rRNA. However, although tRNase Z is conserved in the Thermococcales, the tRNA-like structure is not predicted in the upstream region of either pfu or tko 5S rRNAs (data not shown). EndA is also involved in the removal of rRNA introns45,46,47,48,49, and Nob1 is related to the processing of precursor 16S rRNA in the Archaea20. In summary, this study demonstrates that FAU-1 is important for the growth of T. kodakarensis, and suggests that FAU-1 acts as an RNase and is involved in the regulation of the rRNAs biogenesis and/or turnover in a coordinated manner with several RNases. Though further studies are required for elucidation of rRNA regulation by FAU-1, our findings would lead to a greater understanding of rRNA regulation in Archaea.
Methods
Nucleotide sequencing of P. furiosus precursor 5S rRNAs
Pyrococcus furiosus cells were harvested and suspended in equivalent amounts of RNA buffer (20 mM sodium acetate [pH 5.5], 0.5% SDS, 1 mM EDTA) and acid phenol (pH 5.2). The mixture was incubated at 60 °C for 5 min with gentle shaking. After centrifugation (14,000 rpm for 3 min at room temperature), the supernatant was re-extracted with acid phenol. Total RNA was precipitated with the addition of 2.5 volumes of ethanol to the aqueous phase and incubated for 30 min at −80 °C. The RNA precipitate was collected by centrifugation and dissolved in water. The total RNA was separated on denaturing 6% polyacrylamide gel containing 8 M urea to obtain size-fractionated RNAs (120–500 nt). A cDNA library was prepared using the small RNA Cloning Kit (Takara Bio, Otsu, Shiga, Japan), according to the manufacturer’s protocol. The 5′ precursor DNA sequences of the 5S rRNA were amplified with PCR using a 5′ adaptor primer (S0009) and 5S-rRNA-specific primers (S0010 or S0011) corresponding from nt −5 to +15 of the mature pfu 5S rRNA. The amplified DNA fragments were cloned into the TA cloning vector pMD20 (Takara Bio), and sequenced with an ABI 3130xl Genetic Analyzer (Applied Biosystems, Foster City, CA, USA) with a universal primer (M13 primer M4 5′-GTTTTCCCAGTCACGAC-3′ or M13 primer RV 5′-CAGGAAACAGCTATGAC-3′). The plasmids and primers used in this study are listed in Tables 1 and S2, respectively.
Construction of plasmids encoding recombinant FAU-1 proteins
The construction of plasmid pET-pfu-FAU-1–His has been previously described1. The other plasmids (pET-tko-FAU-1–His, pET-pfu-FAU-1-Δ170–189–His, pET-pfu-FAU-1-Δ190–209–His, and pET-pfu-FAU-1-Δ210–229–His) used in this study were constructed as follows. To construct pET-tko-FAU-1–His, a DNA fragment encoding the tko fau-1 gene (KEGG ID: TK2227) was amplified from the T. kodakarensis chromosomal DNA with PCR and the primer set T0005/T0006 containing NdeI and XhoI sites. The amplified fragment was digested with NdeI and XhoI, and cloned into pET-23b (Novagen, Madison, WI, USA). To construct the internally deleted pfu FAU-1–His plasmids, DNA fragments encoding the internally deleted pfu FAU-1 proteins were amplified from pET-pfu-FAU-1–His with inverse PCR50 with the primer sets P0032/P0031 (pET-pfu-FAU-1-Δ170–189–His), P0034/P0033 (pET-pfu-FAU-1-Δ190–209–His), and P0038/P0039 (pET-pfu-FAU-1-Δ210–229–His). The amplified fragments were self-ligated with the Mighty Cloning Reagent Set (Blunt End) (Takara Bio).
Purification of the FAU-1 proteins
Escherichia coli strain BL21(DE3) was transformed with each plasmid encoding WT FAU-1 or the FAU-1 mutants. The transformants were grown in 200 ml of LB medium containing 50 μg/ml ampicillin at 37 °C to OD600 0.6. After that, isopropyl β-d-thiogalactopyranoside (0.4 mM) was added to the medium, then the samples incubated overnight at 37 °C. After incubation, the cells were harvested by centrifugation (5,000 × g for 5 min at 4 °C) and buffer (50 mM sodium phosphate [pH 7.4], 300 mM NaCl; the recombinant proteins were extracted by sonication (3 min) with 10 mg/ml lysozyme in binding mM imidazole). These extracts were heat treated at 85 °C for 15 min to destroy most of the endogenous proteins, especially RNases, from E. coli, and then centrifuged at 12,000 × g for 10 min at 4 °C to separate the cell debris from the extracts. The supernatants were purified with an Ni2+ affinity chromatography column using the Proteus IMAC Mini Kit (Pro-Chem Inc., Littleton, MA, USA). The bound FAU-1 protein was washed five times with 650 μl of wash buffer (50 mM sodium phosphate [pH 7.4], 300 mM NaCl, 30 mM imidazole) and eluted from the column with 650 μl of elution buffer (50 mM sodium phosphate [pH 7.4], 300 mM NaCl, 300 mM imidazole). To obtain high-purity FAU-1 proteins, these elution fractions were further purified with RESOURCE-Q anion-exchange column chromatography (GE Healthcare, Piscataway, NJ, USA) with a linear gradient of 0–1 M NaCl in Tris-HCl buffer (50 mM Tris-HCl [pH 8.0], 1 mM EDTA, 0.02% Tween 20, 7 mM 2-mercaptoethanol, 10% glycerol). The collected fractions were dialyzed twice with Tris-HCl buffer (50 mM Tris-HCl [pH 8.0], 1 mM EDTA, 0.02% Tween 20, 7 mM 2-mercaptoethanol, 10% glycerol), and analyzed with SDS-polyacrylamide gel electrophoresis and Coomassie Brilliant Blue staining.
In vitro transcription of precursor 5S rRNAs
To construct the plasmids for the in vitro transcription of the precursor 5S rRNAs (pUC-T7-pfu-pre-5S rRNA and pUC-T7-tko-pre-5S rRNA), a DNA fragment containing the 5S rRNA gene (PF_r003 or TK_r01), including its 5′ upstream region (60 bp) with the T7 promoter sequence, was amplified from P. furiosus or T. kodakarensis genomic DNA using the primer set P0044/P0007 or T0009/T0031, respectively. The amplified fragments were cloned into pUC118 digested with HincII using the Mighty Cloning Reagent Set (Blunt End) (Takara Bio).
The pfu or tko 5′ pre-5S rRNA was transcribed with the MEGAshortscript™ T7 Transcription Kit (Thermo Fisher Scientific, Waltham, MA, USA) and the DNA fragment that was amplified from each plasmid (pUC-T7-pfu-pre-5S rRNA or pUC-T7-tko-pre-5S rRNA) with the primer set P0044/P0007 or T0009/T0031, respectively.
In vitro degradation assay
The 0.4 μg or 0.2 μg of transcribed pfu 5′ pre-5S rRNA was incubated with pfu FAU-1 at 65 °C in the presence or absence of each divalent metal ion (Mg2+, Mn2+, or Zn2+) in 20 μl of degradation buffer (15 mM Tris-HCl [pH 8.0], 0.3 mM EDTA, 0.006% Tween 20, 2.1 mM 2-mercaptoethanol, 3% glycerol). The 0.4 μg of tko 5′ pre-5S rRNA was incubated with tko FAU-1 at 52 °C. To stop the reaction, 1 mM EDTA and 40 μl of RNA phenol were immediately added to the reaction mixture. After centrifugation, the supernatants were analyzed by 8M Urea 6% poly acrylamide gel. The 5.0 pmol of A 5′-FITC-labeled RNA probe corresponding to the 5′ precursor region of the pfu 5S rRNA sequences (shown in Fig. 3; (5′-GGCUCUAGAUACUAGCUACAACCGUAAAGUUUUUAAAGUGUUUGUGGGGAGUUGGUAGUGGUACGGCGGCCAU-3′, 73 nt; Hokkaidou System Science, Sapporo, Hokkaido, Japan) was also incubated with pfu FAU-1 at 65 °C in vitro. Then, the samples were analyzed by 8M Urea 20% poly acrylamide gel. The yeast mature tRNA (Invitrogen, Cat. No. 15401–029) was used for a control of in vitro degradation. For phosphatase reaction, the RNA samples were incubated with bacterial alkaline phosphatase (Takara bio) for 30 min at 37 °C.
Construction of a gene disruption plasmid for TK2227
A gene disruption plasmid encoding the tko fau-1 gene (TK2227) (designated pUD3_2227D) was constructed as follows. The TK2227 gene, together with its 5′- and 3′-flanking regions (~1 kbp), was amplified from the T. kodakarensis genomic DNA using the primer set FAU_A5/FAU_A3. The amplified fragment was inserted into the HincII site of pUD3, which carries the tko pyrF gene51. To remove the TK2227 ORF from the resulting plasmid, inverse PCR was performed with the primer set FAU_B5/FAU_B3. The PCR product was self-ligated to construct pUD3_2227D. The absence of unintended mutations was confirmed with DNA sequencing.
Transformation of T. kodakarensis
Thermococcus kodakarensis strains were cultured under strictly anaerobic conditions at 85 °C, in a nutrient-rich medium (ASW-YT) composed of 0.8 × artificial seawater (0.8 × ASW), 5.0 g/l yeast extract, and 5.0 g/l tryptone. In a typical culture, the ASW-YT medium was supplemented with 2.0 g/l elemental sulfur (S0) immediately before inoculation (ASW-YT-S0 medium). A synthetic ASW-AA-S0 medium was also used, consisting of 0.8 × ASW, a mixture of 20 amino acids, modified Wolfe’s trace minerals, a vitamin mixture, and 2.0 g/l elemental sulfur52. In all cases, resazurin was added at a concentration of 0.8 mg/l, and before inoculation, Na2S.9H2O was added to the medium until it became colorless.
Thermococcus kodakarensis strain KUW1 (ΔpyrF·ΔtrpE)28 was used as the host strain for gene disruption. The transformation procedures and selection methods based on uracil prototrophy and resistance to 5-fluoroorotic acid are described elsewhere51. To select and isolate the gene disruptant, ASW-AA-S0 medium was used to enrich the transformants that displayed uracil prototrophy because the transformation plasmid, pUD3_2227D, was integrated into their chromosomes. The solid media used to isolate the gene disruptants were based on ASW-YT medium supplemented with 1.0 g/l gelrite, 0.4 g/l polysulfide, and 7.5 g/l 5-fluoroorotic acid. The genotypes of the isolated transformants were analyzed with PCR and primer set FAU_C5/FAU_C3, which anneals outside of the homologous regions. Transformants whose amplified DNA products were the expected sizes were chosen, and the relevant sequences were confirmed to be as expected. The resulting fau-1-gene-deficient mutants were designated KARNE1 (Δfau-1-n6) and KARNE2 (Δfau-1-n11).
Growth analysis
Pyrococcus furiosus DSM 3638 was grown anaerobically at 96 °C in Pyrococcus/Staphylothermus medium (as in the Handbook of Media for Environmental Microbiology, Second Edition, p. 471). Thermococcus kodakarensis strains KUW1, Δfau-1-n6, and Δfau-1-n11 were also cultured under anaerobic conditions at 93 °C. ASW-YT-Mdx-W medium was used to measure growth, which is based on ASW-YT medium, supplemented with 5.0 g/l maltodextrin and 10 µM Na2WO4. Cell growth was monitored by determining the optical density at 660 nm (OD660).
Northern blotting analysis
Total RNA was extracted from P. furiosus or T. kodakarensis as described above. The total RNA sample was then separated on denaturing 6% polyacrylamide gel containing 8 M urea or denaturing 1.2% agarose gel containing 3.6% formaldehyde, and blotted onto Hybond-N+ membrane (GE Healthcare) with electroblotting or capillary action. After crosslinking, the membrane was prehybridized in DIG Easy Hyb™ buffer (Roche Diagnostics, Mannheim, Germany) for 30 min at 37 °C. A biotin-labeled antisense oligodeoxynucleotide was prepared with the Biotin 3′ End DNA Labeling Kit (Pierce Biotechnology, Rockford, IL, USA), and the membrane was hybridized overnight at 37 °C in DIG Easy Hyb™ buffer containing each labelled antisense probe. The membrane was then washed at 37 °C in buffer containing 30 mM sodium citrate, 3 mM NaCl, and 0.1% SDS. The nonisotopic blots were visualized with the BrightStar® BioDetect™ Kit (Thermo Fisher Scientific) with the ECF Substrate (GE Healthcare). The images were captured with a Molecular Imager FX Pro (Bio-Rad Laboratories, Hercules, CA, USA). The band intensities were analyzed with Molecular Imager FX™ Pro. The pfu 5S rRNA or tko 5S rRNA was detected with DNA probes N0002 (5′-CTTAACTTCCGGGTTCGAAATGAGACCGGGTGTGG-3′) or N0008 (5′-CTTAACTTCCGGGGTCGAAACGAGACCGGGTGTGG-3′), respectively. Either the tko 16S rRNA or 23S rRNA was detected with specific DNA probes (200 bp, 5′terminal sequences) labeled with digoxigenin (DIG) using a PCR DIG Probe Synthesis Kit (Roche Diagnostics, Mannheim, Germany) with the PCR primer pairs: N0009 and N0010, or N0011 and N0012.
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Acknowledgements
We thank all the members of the RNA Group and Dr. Shinnosuke Murakami at the Institute for Advanced Biosciences, Keio University, Japan, for insightful discussions and technical help. We also thank Dr. Carol Mercer and Ms. Kara Wolfe for helpful discussions and critical reading of the manuscript, Dr. Kenji Nakahigashi for valuable discussions and technical help, and Dr. Kahori Ikeda for great help in conducting this study. This research was supported, in part, by research funds from the Yamagata Prefectural Government and Tsuruoka City, Japan, and was supported, in part, by a Grant-in-Aid for Scientific Research (A) (#26242075) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.
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Y.I., Y.O., T.K., H.A., and A.K. conceived and designed the experiments. Y.I. and Y.O. performed the experiments and analyzed the data. A.S., T.K., T.M. and H.A. provided study materials and technical support. Y.I. wrote the main manuscript text. All authors reviewed the manuscript.
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Ikeda, Y., Okada, Y., Sato, A. et al. An archaeal RNA binding protein, FAU-1, is a novel ribonuclease related to rRNA stability in Pyrococcus and Thermococcus . Sci Rep 7, 12674 (2017). https://doi.org/10.1038/s41598-017-13062-3
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DOI: https://doi.org/10.1038/s41598-017-13062-3
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