Vacuole articles within Nature Communications

Featured

• Article
  30 October 2023 | Open Access

  Nanobody-based VSR7 tracing shows clathrin-dependent TGN to Golgi recycling

  Nanobody-epitope interaction-based analysis of the Arabidopsis VACUOLAR SORTING RECEPTOR7 (VSR7) suggests immobilization-based ligand transport to the TGN/EE and ends due to acidity-driven ligand release and clathrin-dependent receptor recycling.

  • Xiaoyu Shao
  • , Hao Xu
  •  & Peter Pimpl

• Article
  10 December 2021 | Open Access

  Spatial control of avidity regulates initiation and progression of selective autophagy

  The molecular principles governing the initiation of autophagosome formation are not clearly understood. Here we show that the vacuolar protein Vac8 coordinates this process by promoting an avidity-driven assembly of several autophagy factors.

  • David M. Hollenstein
  • , Mariya Licheva
  •  & Claudine Kraft

• Article
  24 January 2020 | Open Access

  Dstyk mutation leads to congenital scoliosis-like vertebral malformations in zebrafish via dysregulated mTORC1/TFEB pathway

  Congenital scoliosis is a complex genetic disorder characterized by vertebral malformation. Here, the authors demonstrate that loss of dstyk leads to scoliosis in zebrafish due to dysregulated biogenesis of notochord vacuoles and that DSTYK is required for lysosome biogenesis through mTORC1 regulation.

  • Xianding Sun
  • , Yang Zhou
  •  & Lin Chen

• Article
  13 February 2018 | Open Access

  Nanobody-triggered lockdown of VSRs reveals ligand reloading in the Golgi

  Vacuolar sorting receptors (VSRs) are suggested to efficiently transport hydrolases by continuous cycling. Here, the authors use a nanobody-epitope interaction-based labeling approach to trace VSR recycling from the TGN/EE to the cis-Golgi and reveal ligand reloading of recycled VSRs.

  • Simone Früholz
  • , Florian Fäßler
  •  & Peter Pimpl

• Article
  14 September 2017 | Open Access

  A systematic exploration of the interactions between bacterial effector proteins and host cell membranes

  Microbial pathogens secrete effector proteins into host cells to affect cellular functions. Here, the authors use a yeast-based screen to study around 200 effectors from six bacterial species, showing that over 30% of them interact with the eukaryotic plasma membrane or intracellular organelles.

  • Bethany A. Weigele
  • , Robert C. Orchard
  •  & Neal M. Alto

• Article
  18 August 2017 | Open Access

  Atg4 proteolytic activity can be inhibited by Atg1 phosphorylation

  The protease Atg4 mediates Atg8 lipidation, required for autophagosome biogenesis, but also triggers Atg8 release from the membranes, however is unclear how these steps are coordinated. Here the authors show that phosphorylation by Atg1 inhibits Atg4 at autophagosome formation sites.

  • Jana Sánchez-Wandelmer
  • , Franziska Kriegenburg
  •  & Fulvio Reggiori

• Article
  14 April 2016 | Open Access

  A vacuole-like compartment concentrates a disordered calcium phase in a key coccolithophorid alga

  Coccolithophores are unicellular marine algae that produce calcitic particles inside their cells. Here the authors study cells of the dominant coccolithophore Emiliania huxleyiand identify an intracellular compartment that is filled with high concentrations of a disordered form of calcium.

  • Sanja Sviben
  • , Assaf Gal
  •  & André Scheffel