Review Article |
Featured
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News & Views |
Accelerating maturation of kidney organoids
A two-step method has been developed for the accelerated and efficient generation of human kidney organoids using in vitro and in ovo culture in a three-dimensional environment.
- Susana M. Chuva de Sousa Lopes
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Article |
Fine tuning the extracellular environment accelerates the derivation of kidney organoids from human pluripotent stem cells
A method to accelerate the generation of kidney organoids from human pluripotent stem cells cultured in a three-dimensional environment and exposed to inductive stimuli has been developed, with the organoids capable of recapitulating kidney organogenesis.
- Elena Garreta
- , Patricia Prado
- & Nuria Montserrat
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News & Views |
Mechanics-guided developmental fate patterning
A micropatterned human pluripotent stem cell-based developmental model was utilized to demonstrate the role of biophysical cues such as cell size and cytoskeletal contractile forces in directing patterning of neuroepithelial and neural plate border cells.
- Mukul Tewary
- & Peter W. Zandstra
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Article |
Mechanics-guided embryonic patterning of neuroectoderm tissue from human pluripotent stem cells
Mechanical cues play critical roles in embryonic development. A micropatterned neuroectoderm developmental model based on human pluripotent stem cells now reveals how morophogenetic signals such as cell shape and contractility regulate neural tissue development.
- Xufeng Xue
- , Yubing Sun
- & Jianping Fu
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Letter |
Organoid cystogenesis reveals a critical role of microenvironment in human polycystic kidney disease
Tissue mimics are of great interest in understanding diseases. Here, organoids were developed that resemble polycystic kidney disease cysts and it was demonstrated how material environment and adhesion can affect cystogenesis and disease progression.
- Nelly M. Cruz
- , Xuewen Song
- & Benjamin S. Freedman
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Letter |
Self-organized amniogenesis by human pluripotent stem cells in a biomimetic implantation-like niche
The control of biophysical cues during the culture of human pluripotent stem cells on biomaterial substrates can be used to replicate the in vivo amniogenic environment and direct in vitro generation of early human amniotic tissue.
- Yue Shao
- , Kenichiro Taniguchi
- & Jianping Fu
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News & Views |
Auxetic nuclei
The nuclei of naive mouse embryonic stem cells that are transitioning towards differentiation expand when the cells are stretched and contract when they are compressed. What drives this auxetic phenotype is, however, unclear.
- Ning Wang
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Commentary |
Combining insoluble and soluble factors to steer stem cell fate
Materials-based control of stem cell fate is beginning to be rigorously combined with traditional soluble-factor approaches to better understand the cells' behaviour and maximize their potential for therapy.
- P. C. Dave P. Dingal
- & Dennis E. Discher
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News & Views |
Differential stickiness
Technologies to isolate colonies of human pluripotent stem cells from other cell types in a high-throughput manner are lacking. A microfluidic-based approach that exploits differences in the adhesion strength between these cells and a substrate may soon fill the gap.
- Oscar J. Abilez
- & Joseph C. Wu
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Article |
Combinatorial development of biomaterials for clonal growth of human pluripotent stem cells
Structure–property relationships between material properties and stem cell behaviour are investigated using high-throughput methods. The data identify the optimal substrates within a range of different polymeric surfaces to support the growth and self-renewal of human embryonic stem cells from fully dissociated single cells.
- Ying Mei
- , Krishanu Saha
- & Daniel G. Anderson
Rethinking organoid technology through bioengineering