Featured
-
-
Article
| Open AccessA phosphoinositide hub connects CLE peptide signaling and polar auxin efflux regulation
In this paper the authors demonstrate that auxin efflux in the phloem vascular tissue of plants is determined by antagonism between CLE peptide signaling pathways and phosphoinositide kinases.
- Qian Wang
- , A. Cecilia Aliaga Fandino
- & Christian S. Hardtke
-
Article
| Open AccessINPP4B promotes PI3Kα-dependent late endosome formation and Wnt/β-catenin signaling in breast cancer
The PI(3,4)P2 4-phosphatase, INPP4B, functions as a tumour suppressor in triple negative breast cancer. Here, the authors show that INPP4B enhances proliferation and growth of PIK3CA-mutant ER+ breast cancers by promoting PI3Kα-dependent late endosome formation and trafficking that leads to the activation of Wnt/β-catenin signalling.
- Samuel J. Rodgers
- , Lisa M. Ooms
- & Christina A. Mitchell
-
Article
| Open AccessTNFAIP8 controls murine intestinal stem cell homeostasis and regeneration by regulating microbiome-induced Akt signaling
The molecular mechanisms that regulate intestinal Clu+ revival stem cells (revSCs) and their niche to enable regeneration in response to injury are unclear. Here, the authors show that mice without the phospholipid transport protein, TNFAIP8, causes less revSCs to be induced following injury.
- Jason R. Goldsmith
- , Nina Spitofsky
- & Youhai H. Chen
-
Article
| Open AccessThe phospholipid PI(3,4)P2 is an apical identity determinant
During de novo establishment of apical-basal polarity, a basolateral membrane must be converted into an apical delivery zone. Here, the authors use MDCK 3D cysts to uncover that the phospholipid PI(3,4)P2 is an apical membrane determinant.
- Álvaro Román-Fernández
- , Julie Roignot
- & David M. Bryant
-
Article
| Open AccessMutual inhibition between PTEN and PIP3 generates bistability for polarity in motile cells
PIP3 and its phosphatase (PTEN) are enriched mutually exclusively on the anterior and posterior membranes of eukaryotic motile cells. Here authors manipulate PIP3 level and use single-molecule imaging to show that PIP3 suppresses the membrane localization of PTEN.
- Satomi Matsuoka
- & Masahiro Ueda
-
Article
| Open AccessDNA damage causes rapid accumulation of phosphoinositides for ATR signaling
Phosphoinositides are enriched in the nucleus and accumulate upon DNA damage but their role in responding to DNA damage is poorly defined. Here, the authors show that phosphoinositides rapidly accumulate at DNA damage sites and are required for ATR recruitment and subsequent Chk1 activation.
- Yu-Hsiu Wang
- , Anushya Hariharan
- & Michael P. Sheetz
-
Article
| Open AccessSurvival of pancreatic cancer cells lacking KRAS function
Pancreatic cancer cells may develop resistance to KRAS inhibitors due to activation of compensatory pathways. In this study, the authors demonstrate that KRAS is dispensable in a subset of pancreatic cancer and that PI3K signalling may have an important role in mediating tumor growth following KRAS inhibition.
- Mandar Deepak Muzumdar
- , Pan-Yu Chen
- & Tyler Jacks
-
Article
| Open AccessAGC family kinase 1 participates in trogocytosis but not in phagocytosis in Entamoeba histolytica
Entamoeba histolytica can kill host cells by trogocytosis, while it ingests dead cells by phagocytosis. Here, Somlata et al. show that EhAGCK1, an AGC family kinase, is specifically involved in trogocytosis, shedding light on the molecular differences between trogocytosis and phagocytosis.
- Somlata
- , Kumiko Nakada-Tsukui
- & Tomoyoshi Nozaki
-
Article
| Open AccessDeconvolution of Buparlisib’s mechanism of action defines specific PI3K and tubulin inhibitors for therapeutic intervention
Buparlisib/BKM120 is in phase 3 clinical trials as a phosphoinositide 3-kinase (PI3K) inhibitor. Here, Bohnackeret al. combine chemical biology and structural biology approaches to segregate BKM120’s biological actions, and suggest that it causes mitotic arrest predominantly by binding microtubules and disrupting their dynamics.
- Thomas Bohnacker
- , Andrea E. Prota
- & Matthias P. Wymann
-
Article
| Open AccessPI(4,5)P2 5-phosphatase A regulates PI3K/Akt signalling and has a tumour suppressive role in human melanoma
Inositol polyphosphate 5-phosphatases, such as PIB5PA, terminate signalling downstream of phosophoinositide-3 kinase; however, their biological roles remain unclear. Here the authors report that PIB5PA has a tumour suppressive role in melanoma.
- Yan Ye
- , Lei Jin
- & Xu Dong Zhang