Featured
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Article |
Structures of rhodopsin in complex with G-protein-coupled receptor kinase 1
Cryo-EM structures of complexes between GRK1 and rhodopsin shed light on how a small number of GRKs can selectively recognize and be activated by hundreds of different G-protein-coupled receptors.
- Qiuyan Chen
- , Manolo Plasencia
- & John J. G. Tesmer
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Letter |
Cryo-EM of the dynamin polymer assembled on lipid membrane
A cryo-electron microscopy structure of human dynamin-1 demonstrates conformational changes and sheds light on the fission of membranes during endocytosis.
- Leopold Kong
- , Kem A. Sochacki
- & Jenny E. Hinshaw
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Letter |
Dynamics of phosphoinositide conversion in clathrin-mediated endocytic traffic
‘Coincidence-detecting’ phosphoinositide sensors are used to study changes in the phosphoinositide lipid species found in membranes during the development and maturation of endocytic clathrin-coated vesicles.
- Kangmin He
- , Robert Marsland III
- & Tom Kirchhausen
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Letter |
Hemi-fused structure mediates and controls fusion and fission in live cells
Super-resolution imaging provides direct evidence in live cells that membrane fusion and fission are mediated through an intermediate hemi-fused structure, where fusion and calcium/dynamin-dependent fission mechanisms compete to determine the transition of the intermediate to fusion or fission.
- Wei-Dong Zhao
- , Edaeni Hamid
- & Ling-Gang Wu
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Letter |
Polarized endosome dynamics by spindle asymmetry during asymmetric cell division
Central spindle asymmetry, generated by the kinesin Klp10A and its antagonist Patronin, polarizes endosome motility and provides a mechanism for the asymmetric segregation of signalling endosomes observed in a variety of asymmetrically dividing cell types.
- Emmanuel Derivery
- , Carole Seum
- & Marcos Gonzalez-Gaitan
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Letter |
A hemi-fission intermediate links two mechanistically distinct stages of membrane fission
The GTPase dynamin provides the driving force for fission of membrane-bound vesicular structures; here, it is shown that dynamin-driven membrane fission proceeds in two mechanistically distinct stages that are separated by a metastable hemi-fission intermediate that requires GTP hydrolysis for progression to full fission.
- Juha-Pekka Mattila
- , Anna V. Shnyrova
- & Vadim A. Frolov
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Letter |
Endophilin-A2 functions in membrane scission in clathrin-independent endocytosis
Endophilin-A2 (endoA2) is shown to mediate clathrin-independent endocytosis of Shiga and cholera toxins, and to function in parallel with dynamin and actin in the pulling-force-driven scission of Shiga-toxin-induced tubular structures.
- Henri-François Renard
- , Mijo Simunovic
- & Ludger Johannes
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Article |
Endophilin marks and controls a clathrin-independent endocytic pathway
This study describes a fast, clathrin-independent endocytic pathway mediated by endophilin, dynamin and actin; the pathway is activated by ligand binding to a variety of cargo receptors, and endophilin-mediated endocytosis occurs primarily at the leading edges of cells where lamellipodin and the lipid PtdIns(3,4)P2 ensure endophilin targeting.
- Emmanuel Boucrot
- , Antonio P. A. Ferreira
- & Harvey T. McMahon
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Article |
Clathrin regenerates synaptic vesicles from endosomes
Ultrastructural analysis of synaptic vesicle recycling reveals that clathrin is not required for the initial rapid step of vesicle recycling by ultrafast endocytosis at the plasma membrane and instead clathrin acts later at an endosome to regenerate synaptic vesicles; however, when ultrafast endocytosis does not occur (for example, in experiments at room temperature rather than physiological temperature), clathrin-mediated endocytosis does happen at the plasma membrane.
- Shigeki Watanabe
- , Thorsten Trimbuch
- & Erik M. Jorgensen
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Article |
Ultrafast endocytosis at mouse hippocampal synapses
Sustained neurotransmission requires recycling of synaptic vesicles, but the proposed mechanisms have been controversial; here a ‘flash-and-freeze’ method for electron microscopy reveals a new ultrafast form of endocytosis that is actin- and dynamin-dependent and occurs within 100 milliseconds of stimulation.
- Shigeki Watanabe
- , Benjamin R. Rost
- & Erik M. Jorgensen
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Letter |
Spatiotemporal control of endocytosis by phosphatidylinositol-3,4-bisphosphate
Phosphoinositides are important regulators of intracellular membrane traffic, and although the role of PI(4,5)P2 has been well characterised, the function of PI(3,4)P2 remains unclear; here the formation of PI(3,4)P2 by the class II phosphatidylinositol-3-kinase C2α enzyme is shown to control clathrin-mediated endocytosis.
- York Posor
- , Marielle Eichhorn-Gruenig
- & Volker Haucke
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Letter |
Tumour suppressor RNF43 is a stem-cell E3 ligase that induces endocytosis of Wnt receptors
In vivo and in vitro studies show that the stem-cell E3 ubiquitin ligases RNF43 and ZNRF3 act as tumour suppressors in colorectal cancer models, and are involved in the negative regulation of the cancer-associated Wnt signalling pathway through limiting the cell-surface expression of Wnt receptors.
- Bon-Kyoung Koo
- , Maureen Spit
- & Hans Clevers
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News & Views |
How to don a coat
Cargo-carrying vesicles can assemble from hundreds of locations on the cell membrane, but how these sites are selected has been unclear. A small family of membrane-sculpting proteins may select the perfect location.
- Linton M. Traub
- & Beverly Wendland
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Article |
Systems survey of endocytosis by multiparametric image analysis
A new strategy is presented to accurately profile the activity of human genes in endocytosis by combining genome-wide RNAi, automated high-resolution confocal microscopy and quantitative multi-parametric image analysis. Several novel components of endocytosis and endosome trafficking were uncovered; a systems analysis further revealed that the cell regulates the number, size and concentration of cargo within endosomes.
- Claudio Collinet
- , Martin Stöter
- & Marino Zerial
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